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Sterile culture of the free-living stages of the sheep stomach worm, Haemonchus contortus

Published online by Cambridge University Press:  06 April 2009

R. W. Glaser
Affiliation:
From the Department of Animal and Plant Pathology, The Rockefeller Institute for Medical Research, Princeton, N.J.
Norman R. Stoll
Affiliation:
From the Department of Animal and Plant Pathology, The Rockefeller Institute for Medical Research, Princeton, N.J.

Extract

The effect of an efficient bactericidal solution on the hatching of Haemonchus eggs was studied and it was found that a large proportion remained viable. Based on former work, a medium was devised in which the bacteria-free eggs hatched and the larvae developed to the infective stage. These larvae were morphologically and otherwise normal, except that they were slightly smaller than faecal-grown specimens, although the size ranges overlapped. Infection tests proved that Haemonchus larvae grown under conditions of sterility were normally infective for, and produced normal adults in, a susceptible lamb.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1938

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