Hostname: page-component-77c89778f8-gvh9x Total loading time: 0 Render date: 2024-07-21T05:54:18.106Z Has data issue: false hasContentIssue false

Sterile culture of the free-living stages of the sheep stomach worm, Haemonchus contortus

Published online by Cambridge University Press:  06 April 2009

R. W. Glaser
From the Department of Animal and Plant Pathology, The Rockefeller Institute for Medical Research, Princeton, N.J.
Norman R. Stoll
From the Department of Animal and Plant Pathology, The Rockefeller Institute for Medical Research, Princeton, N.J.


The effect of an efficient bactericidal solution on the hatching of Haemonchus eggs was studied and it was found that a large proportion remained viable. Based on former work, a medium was devised in which the bacteria-free eggs hatched and the larvae developed to the infective stage. These larvae were morphologically and otherwise normal, except that they were slightly smaller than faecal-grown specimens, although the size ranges overlapped. Infection tests proved that Haemonchus larvae grown under conditions of sterility were normally infective for, and produced normal adults in, a susceptible lamb.

Research Article
Copyright © Cambridge University Press 1938

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)



Davey, D. G. (1937). Physiology of nematodes. Nature, Lond., 140, 645.CrossRefGoogle Scholar
Dikmans, G. & Andrews, J. S. (1933). A comparative morphological study of the infective larvae of the common nematodes parasitic in the alimentary tract of sheep. Trans. Amer. micr. Soc. 52, 125.CrossRefGoogle Scholar
Glaser, R. W. & Coria, N. A. (1933). The culture of Paramecium caudatum free from living micro-organisms. J. Parasit. 20, 33–7.Google Scholar
Glaser, R. W. & Coria, N. A. (1935). The culture and reactions of purified protozoa. Amer. J. Hyg. 21, 111–20.Google Scholar
Glaser, R. W. (1938). A method for the culture of sterile houseflies. J. Parasit. 24, 177–9.Google Scholar
Hoeppli, R. (19361937). Modern trends in parasitology. Peking nat. Hist. Bull. 11, pt. 3, 257–80.Google Scholar
Lapage, G. (1933). The cultivation of infective nematode larvae on cultures of Bacillus coli. Univ. Cambridge, 3rd Rep. Dir., Inst. Animal Path. pp. 237–71.Google Scholar
McCoy, O. R. (1929 a). The growth of hookworm larvae on pure cultures of bacteria. Science, N.S. 69, 74–5.CrossRefGoogle ScholarPubMed
McCoy, O. R. (1929 b). The suitability of various bacteria as food for hookworm larvae. Amer. J. Hyg. 10, 140–56.Google Scholar
McCoy, O. R. (1930). The influence of temperature, hydrogen-ion concentration, and oxygen tension on the development of the eggs and larvae of the dog hookworm, Ancylostoma caninum. Amer. J. Hyg. 11, 413–48.Google Scholar
Mönnig, H. O. (1931). The specific diagnosis of nematode infestation in sheep. 17th Ann. Rep., Dir. Vet. Services and Animal Industry, Union South Africa, Dept. Agric., pp. 255–64.Google Scholar
Ransom, B. H. (1906). The life history of the twisted wireworm (Haemonchus contortus) of sheep and other ruminants. (Preliminary Rep.) Bur. Animal Industry, U.S. Dept. Agric., Circ. 93, pp. 17.Google Scholar
Stoll, N. R. (1923). Investigations on the control of hookworm disease. XVII. A quantitative study defining a point of breakdown of hookworm eggs cultured in faeces, and its association with intense acidity. Amer. J. Hyg. 3, 137–55.Google Scholar
Stoll, N. R. (1930). On methods of counting nematode ova in sheep dung. Parasitology, 22, 116–36.CrossRefGoogle Scholar
Trager, W. (1935 a). The culture of mosquito larvae free from micro-organisms. Amer. J. Hyg. 22, 1825.Google Scholar
Trager, W. (1935 b). On the nutritional requirements of mosquito larvae (Aedes aegypti). Amer. J. Hyg. 22, 475–93.Google Scholar
Trager, W. (1937). A growth factor required by mosquito larvae. J. exp. Biol. 14, 240–51.CrossRefGoogle Scholar
Veglia, F. (1915). The anatomy and life history of the Haemonchs contortus (Rud.). 3rd and 4th Rep. Dir. Vet. Research, Dept. Agric., Onderstepoort, Union of South Africa, pp. 349500.Google Scholar
Wardle, R. A. (1937). The physiology of the sheep tapeworm, Moniezia expansa Blanchard. Canad. J. Res. 15, Section D, pp. 117–26.Google Scholar
White, G. F. (1931). Production of sterile maggots for surgical use. J. Parasit. 18, 133.Google Scholar