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Schistosoma mansoni: stage-dependent membrane vesiculation and parasite killing induced by a cytotoxin from Pseudomonas aeruginosa

Published online by Cambridge University Press:  06 April 2009

A. Ruppel ,
R. Breternitz  and
F. Lutz
A. Ruppel*
Affiliation:
Institut für Tropenhygiene, Universität Heidelberg, FRG
R. Breternitz
Affiliation:
Institut für Tropenhygiene, Universität Heidelberg, FRG
F. Lutz
Affiliation:
Institut für Pharmakologie und Toxikologie, Universität Giessen, FRG
*
*Reprint requests: Dr A.Ruppel, Institut für Tropenhygiene, Im Neuenheimer Feld 324, D-6900 Heidelberg, Federal Republic of Germany.
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Summary

We have examined the interaction between Schistosoma mansoni and a cytotoxin from Pseudomonas aeruginosa known to affect a variety of cell types through the formation of membrane pores. The killing effect of the cytotoxin on S. mansoni in vitro was strongly dependent on the parasite developmental stage. Skin schistosomula were most sensitive, while 4-week-old or older parasites were less so. In contrast, lung schistosomula were relatively resistant and juvenile mesenteric forms younger than 28 days were almost completely refractory to the action of the toxin. A sharp increase in sensitivity to the toxin was observed between 27 and 28 days of parasite age with a parasite length of about 1·80 mm appearing as the threshold. Thus, the schistosome sensitivity to the cytotoxin changed during parasite development in a way similar to the known sensitivity of the worms to in vitro immune attack. Killing of the parasites by the cytotoxin was preceded by the formation of vesicles which, in the case of adult worms, detached easily from the parasites. Evidence obtained by electron microscopy and by immunofluorescent assays with living worms demonstrated that the outer parasite membrane participated in the formation of the vesicle membrane. Thus, the cytotoxin may be a useful tracer for changes in the properties of the schistosome surface membrane during parasite development.

Type
Research Article
Information
Parasitology , Volume 95 , Issue 2 , October 1987 , pp. 241 - 251
Copyright
Copyright © Cambridge University Press 1987

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