Hostname: page-component-77c89778f8-m42fx Total loading time: 0 Render date: 2024-07-23T01:04:05.328Z Has data issue: false hasContentIssue false
  • English
  • Français

Protein analysis of Theileria sergenti/buffeli/orientalis piroplasms by two-dimensional polyacrylamide gel electrophoresis

Published online by Cambridge University Press:  06 April 2009

C. Sugimoto ,
S. Kawazu ,
T. Kamio  and
K. Fujisaki
C. Sugimoto
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
S. Kawazu
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
T. Kamio
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
K. Fujisaki
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
Get access Rights & Permissions [Opens in a new window]

Extract

Proteins of the piroplasms of Theileria sergenti, T. buffeli and T. orientalis were analysed by two-dimensional polyacrylamide gel electrophoresis. Protein spot patterns of T. buffeli and T. orientalis were identical except for a few minor proteins, whereas spot patterns of two T. sergenti stocks were differentiated from those of T. buffeli and T. orientalis by a characteristic set of proteins including a major protein of molecular weight 33–34 kDa. This result indicates that Japanese T. sergenti can be phenotypically distinguishable from European and Australia Theileria species; T. orientalis and T. buffeli.

Keywords

Theileria sergentiTheileria buffeliTheileria orientalistwo-dimensional gel electrophoresis
Type
Research Article
Information
Parasitology , Volume 102 , Issue 3 , June 1991 , pp. 341 - 346
Copyright
Copyright © Cambridge University Press 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anderson, N. G. & Anderson, N. L. (1978 a). Analytical techniques for cell fractions: XXI. Two-dimensional analysis of serum and tissue proteins: multiple isoelectric focusing. Analytical Biochemistry 85, 331–40.Google Scholar
Anderson, N. L. & Anderson, N. G. (1978 b). Analytical techniques for cell fractions: XXII. Two-dimensional analysis of serum and tissue proteins: multiple gradient slab gel electrophoresis. Analytical Biochemistry 85, 341–54.Google Scholar
Anderson, N. L., Parish, N. M., Richardson, J. P. & Pearson, T. W. (1985). Comparison of African trypanosomes of different antigenic phenotypes, subspecies, and life cycle stages by two-dimensional gel electrophoresis. Molecular and Biochemical Parasitology 16, 299–314.CrossRefGoogle ScholarPubMed
Callow, L. L. (1984). Theileriosis. In Animal Health in Australia, vol. 5, pp. 168–73. Australian Government Publishing Service, Canberra.Google Scholar
Edwards, J. J., Anderson, N. G., Nance, S. L. & Anderson, N. L. (1979). Red cell proteins. I. Two-dimensional mapping of human erythrocyte lysate proteins. Blood 53, 1121–32.CrossRefGoogle ScholarPubMed
Fujisaki, K., Ito, Y., Kamio, T. & Kitaoka, S. (1985). The presence of Theileria sergenti in Haemaphysalis longicornis overwintering in pasture in Japan. Annals of Tropical Medicine and Parasitology 79, 519–34.Google Scholar
Fujisaki, K., Kamio, T. & Kawazu, S. (1991). Theileria sergenti cannot be regarded as the same species as T. buffeli and T. orientalis because of its transmissibility only by Kaiseriana ticks. In Modern Acarology (ed. Dusbabek, F.). Publishing House Academia, Prague (in the Press).Google Scholar
Glascodine, J., Tetley, L., Tait, A., Brown, D. & Shields, B. (1990). Developmental expression of Theileria annulata merozoite surface antigen. Molecular and Biochemical Parasitology 40, 105–12.Google Scholar
Howard, R. J., Aley, S. B. & Lemkin, P. F. (1983). High resolution comparison of Plasmodium knowlesi clones of different variant antigen phenotypes by two-dimensional gel electrophoresis and computer analysis. Electrophoresis 4, 420–7.Google Scholar
Katende, J. M., Goddeeris, B. M., Morzaria, S. P., Nkonge, C. G. & Musoke, A. J. (1990). Identification of a Theileria mutans-specific antigen for use in an antibody and antigen detection ELISA. Parasite Immunology 12, 419–33.Google Scholar
Kobayashi, N., Onuma, M., Kirisawa, R., Ohgitani, T., Takahashi, K., Sasaki, N. & Kawakami, Y. (1987). Monoclonal antibodies against intraerythrocytic merozoites (piroplasms) of Theileria sergenti. Japanese Journal of Veterinary Medicine 49, 697–702.Google ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Minami, T., Fujinaga, T., Furuya, K. & Ishiwara, T. (1980). Clinico-hematologic and serological comparisons of Japanese and Russian strains of Theileria sergenti. National Institute of Animal Health Quarterly 20, 44–52.Google Scholar
Minami, T., Kawazu, S., Shimura, K., Fujisaki, K., Kamio, T. & Ito, S. (1990). Detection of the schizont stage in experimentally infected cattle with Japanese Theileria sergenti. Japanese Journal of Veterinary Science 52, 601–4.Google ScholarPubMed
O'Farrell, P. H. (1975). High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250, 4007–21.Google Scholar
O'Farrell, P. Z., Goodman, H. M. & O'Farrell, P. H. (1977). High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12, 1133–42.CrossRefGoogle ScholarPubMed
Ohgitani, T., Okabe, T. & Sasaki, N. (1987). Antigenic properties of Theileria sergenti in serodiagnosis. Japanese Journal of Veterinary Science 49, 531–4.Google ScholarPubMed
Stewart, N. P., De Vos, A. J., Shiels, I. & McGregor, W. (1987). The experimental transmission of Theileria buffeli of cattle in Australia by Haemaphysalis humerosa. Australian Veterinary Journal 64R, 81–3.CrossRefGoogle ScholarPubMed
Stewart, N. P., De Vos, A. J., McGregor, W. & Shiels, I. (1988). Observations on the development of tick-transmitted Theileria buffeli (syn T. orientalis?) in cattle. Research in Veterinary Science 44, 338–42.Google Scholar
Sugimoto, C., Conrad, P. A., Mutharia, L., Dolan, T. T., Brown, W. C., Goddeeris, B. M. & Pearson, T. W. (1989). Phenotypic characterization of Theileria parva schizonts by two-dimensional gel electrophoresis. Parasitology Research 76, 1–7.Google Scholar
Sugimoto, C., Sato, M., Kawazu, S., Kamio, T. & Fujisaki, K. (1991). Purification of merozoites of Theileria sergenti from infected bovine erythrocytes. Parasitology Research (in the Press).CrossRefGoogle ScholarPubMed
Sutton, C. A., Shirley, M. W. & Wisher, M. H. (1989). Characterization of coccidial proteins by two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Parasitology 99, 175–87.CrossRefGoogle ScholarPubMed
Tait, A. (1981). Analysis of protein variation in Plasmodium falciparum by two-dimensional gel electrophoresis. Molecular and Biochemical Parasitology 2, 205–18.CrossRefGoogle ScholarPubMed
Uilenberg, G. (1981). Theilerial species of domestic livestock. In Advances in the Control of Theileriosis. (ed. Irvin, A. D., Cunningham, M. P. & Young, A. S.), pp. 4–37. The Hague: Martinus Nijhoff.CrossRefGoogle Scholar
Uilenberg, G., Perie, N. M., Spanjer, A. A. M. & Franssen, F. F. J. (1985). Theileria orientalis, a cosmopolitan blood parasite of cattle: demonstration of the schizont stage. Research in Veterinary Science 38, 352–7.CrossRefGoogle ScholarPubMed