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A monoclonal antibody to Babesia divergens which inhibits merozoite invasion

Published online by Cambridge University Press:  06 April 2009

Caroline M. Winger
Affiliation:
Department of Pure and Applied Biology, Imperial College, London SW7 2BB
Elizabeth U. Canning
Affiliation:
Department of Pure and Applied Biology, Imperial College, London SW7 2BB
J. D. Culverhouse
Affiliation:
Department of Pure and Applied Biology, Imperial College, London SW7 2BB

Summary

A monoclonal antibody-producing hybridoma cell line has been raised against merozoites of Babesia divergens. This antibody is strongly reactive against merozoites in an enzyme-linked immunosorbent assay (ELISA) and is also positive in an indirect fluorescent antibody test (IFAT) on preparations of live merozoites. The antibody recognizes an antigen, of molecular weight (Mr) 50–60 K by Western blot analysis on SDS–polyacrylamide gel electrophoresis (SDS–PAGE) gels. Merozoite neutralization assays show that the antibody significantly inhibits merozoite invasion of bovine erythrocytes.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1987

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References

Grothaus, G. D. & Kreier, J. P. (1980). The isolation of a soluble component of Plasmodium berghei which induces immunity in rats. Infection and Immunity 28, 245–53.CrossRefGoogle ScholarPubMed
Harte, P. G., Rogers, N. & Targett, G. A. T. (1985). Vaccination with purified microgamete antigens prevents transmission of rodent malaria. Nature, London 316, 258.CrossRefGoogle ScholarPubMed
James, M. A., Levy, M. G. & Ristic, M. (1981). Isolation and partial characterization of culture derived soluble Babesia bovis antigens. Infection and Immunity 31, 358–61.CrossRefGoogle ScholarPubMed
Kennett, R. (1979). Cell fusion. In Methods in Enzymology (ed. Jakoby, W. and Pastan, J.), pp. 345–9. New York and London: Academic Press.Google Scholar
Köhler, G. & Milstein, C. (1975). Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256, 495–7.CrossRefGoogle ScholarPubMed
Konrad, J., Phipps, L. P., Canning, E. U. & Donnelly, J. (1984). Long term in vitro maintenance of Babesia divergens in a stationary phase culture. Parasitology 89, lxxvi.Google Scholar
Kuttler, K. L., Levy, M. G., James, M. A. & Ristic, M. (1982). Efficacy of a non-viable culture derived Babesia bovis vaccine. American Journal of Veterinary Research 43, 281–4.Google Scholar
Kuttler, K. L., Levy, M. G. & Ristic, M. (1983). Cell culture derived Babesia bovis vaccine: sequential challenge exposure of protective immunity during a 6 month post vaccination period. American Journal of Veterinary Research 44, 1456–9.Google Scholar
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature, London 277, 680–5.CrossRefGoogle Scholar
Levy, M. G. & Ristic, M. (1980). Babesia bovis: continuous cultivation in a microaerophilus stationary phase culture. Science 207, 1218–20.CrossRefGoogle Scholar
Mahoney, D. F. (1967). Bovine babesiosis: the immunisation of cattle with killed Babesia argentina. Experimental Parasitology 20, 125–9.CrossRefGoogle ScholarPubMed
Mahoney, D. F. & Goodger, B. V. (1972). Babesia argentina: immunogenicity of plasma from infected animals. Experimental Parasitology 32, 7185.CrossRefGoogle ScholarPubMed
Miller, L. H., David, P. H. & Hadley, T. J. (1983). The surface membrane of Plasmodium: events tracing the invasive merozoites. Proceedings of the 2nd International Conference of Malaria and Babesiosis, Annecy, France, p. 29 (abstract).Google Scholar
Molinar, E., James, M. A., Kakoma, I., Holland, C. J. & Ristic, M. (1982). Antigenic and immunogenic studies on cell culture derived Babesia canis. Veterinary Parasitology 10, 2940.CrossRefGoogle ScholarPubMed
Ristic, M. (1981). Babesiosis. In Disease of Cattle in the Tropics (ed. Ristic, M. and McIntyre, I.), pp. 443–68. The Hague: Martinus Nijhoff.Google Scholar
Ristic, M. (1984). Research on babesiosis vaccines. In Malaria and Babesiosis (ed. Ristic, M., P., Ambroise-Thomas and Kreier, J. P.), pp. 103–23. The Hague: Martinus Nijhoff.CrossRefGoogle Scholar
Ristic, M. & Levy, M. G. (1981). A new era of research toward solution of bovine Babesiosis. In Babesiosis (ed. Ristic, M. and Kreier, J. P.), pp. 509–44. New York: Academic Press.Google Scholar
Ristic, M., Smith, R. D. & Kakoma, I. (1981). Characterization of Babesia antigens derived from cell cultures and ticks. In Babesiosis (ed. Ristic, M. and Kreier, J. P.), pp. 337–80. New York: Academic Press.Google Scholar
Saul, K. W. & Kreier, J. P. (1977). Plasmodium berghei: immunisation of rats with antigens from a population of free parasites rich in merozoites. Annals of Tropical Medicine and Parasitology 28, 302–18.Google Scholar
Smith, R. D., James, M. A., Ristic, M., Aikawa, M. & Vega, C. A. (1981). Bovine babesiosis: protection of cattle with culture derived soluble Babesia bovis antigen. Science 212, 335–8.CrossRefGoogle ScholarPubMed
Smith, R. D. & Ristic, M. (1981). Immunisation against bovine babesiosis with culture derived antigens. In Babesiosis (ed. Ristic, M. and Kreier, J. P.), pp. 485507. New York: Academic Press.Google ScholarPubMed
Timms, P. & Stewart, N. P. (1984). Immune responses of cattle following vaccination with living and non living Babesia bovis antigens. Veterinary Parasitology 16, 243–51.CrossRefGoogle ScholarPubMed
Väyrynen, R. & Tuomi, J. (1982). Continuous in vitro cultivation of Babesia divergens. Acta Veterinaria Scandinavica 23, 471–2.CrossRefGoogle ScholarPubMed
Voller, A., Bidwell, D. E. & Bartlett, A. (1976). Enzyme immunoassays in diagnostic medicine. Theory and Practice. Bulletin of the World Health Organization 53, 3418–21.Google ScholarPubMed
Wright, I. G., White, M., Tracey-Patte, P. D., Donaldson, R. A., Goodger, B. V., Waltisbuhl, D. J. & Mahoney, D. F. (1983). Babesia bovis isolation of a protective antigen by using monoclonal antibodies. Infection and Immunity 41, 244–50.CrossRefGoogle ScholarPubMed
Wright, I. G., Mirre, G. B., Rode-Bramanis, K., Chamberlain, M., Goodger, B. V. & Waltisbuhl, D. J. (1985). Protective vaccination against virulent Babesia bovis with a low-molecular-weight antigen. Infection and Immunity 48, 109–13.CrossRefGoogle ScholarPubMed