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Eimeria tenella in chickens: development of resistance to quinolone anticoccidial drugs

Published online by Cambridge University Press:  06 April 2009

H. D. Chapman
H. D. Chapman
Affiliation:
Houghton Poultry Research Station, Houghton, Huntingdon, Cambs.
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Extract

The development of drug resistance by the present Houghton strain of Eimeria tenella to the quinolones, methyl benzoquate and buquinolate, was found to take place after a single experimental passage. The development of resistance was independent of drug selection pressure and showed cross resistance to other quinolones, but not to amprolium and robenidine. When the Weybridge, Beltsville and Elberfeld strains of E. tenella were compared under similar laboratory conditions, the Weybridge and Elberfeld strains developed resistance to methyl benzoquate after 6 passages and the Beltsville after 5. Studies on the response of the Houghton strain to methyl benzoquate and buquinolate revealed that the drugs did not completely control the infection as measured by weight gain and that oocyst production was not suppressed. These observations indicate that the strain had already acquired some resistance to these drugs. This was confirmed by examining the resistance to methyl benzoquate of a culture of the Houghton strain of E. tenella which had been stored frozen in liquid nitrogen since 1969. This showed full sensitivity to the drug and developed resistance after 8 passages. This suggests that drug tolerance has been acquired by the Houghton strain since 1969.

Oocyst lines were established from the Houghton strain by infecting single birds with approximately 10 oocysts. Eleven of these lines were found to be sensitive to methyl benzoquate, and nine to give rise to resistant parasites. It is concluded that the Houghton strain is contaminated by a small number of resistant oocysts which can be eliminated from a culture by dilution of the challenge inoculum. One of these Houghton oocyst lines, sensitive to methyl benzoquate, developed resistance after 8 serial passages.

Type
Research Article
Information
Parasitology , Volume 71 , Issue 1 , August 1975 , pp. 41 - 49
Copyright
Copyright © Cambridge University Press 1975

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References

Chapman, H. D. (1974). Use of chick embryo infections for the development of drug resistance in Eimeria tenella. Parasitology (in the Press).CrossRefGoogle Scholar
Foure, N. & Bennejean, G. (1973). Les chimioresistances acquises vis a vis des derives des quinoleines et de la pyridine. Symposium international sur les coccidioses, Tours 1973.Google Scholar
Jeffers, T. K. (1974). Eimeria tenella: Incidence, distribution and anti-coccidial drug resisance of isolants in major broiler producing areas. Avian Diseases 18, 74–84.CrossRefGoogle Scholar
Joyner, L. P. (1970). Coccidiosis: problems arising from the development of anticoccidial drug resistance. Experimental Parasitology 28, 122–8.CrossRefGoogle ScholarPubMed
Joyner, L. P. & Norton, C. C. (1973). The immunity arising from continuous low level in fection with Eimeria tenella. Parasitology 67, 333–40.CrossRefGoogle Scholar
Long, P. L. (1968). The pathogenic effects of Eimeria praecox and E. acervulina in the chicken. Parasitology 58, 691–700.CrossRefGoogle Scholar
Long, P. L. (1972). Eimeria tenella: Reproduction, Pathogenicity and immunogenicity of a strain maintained in chick embryos by serial passage. Journal of Comparative Pathology 82, 429–37.CrossRefGoogle ScholarPubMed
Long, P. L. & Rowell, J. C. (1958). Counting oocysts of chicken coccidia. Laboratory Practice 7, 515–9.Google Scholar
McLoughlin, D. K. (1970). Efficacy of buquinolate against ten strains of Eimeria tenella and the development of a resistant strain. Avian Diseases 14, 126–30.CrossRefGoogle ScholarPubMed
McLoughlin, D. K. & Chute, M. B. (1971). Efficacy of decoquinate against eleven strains of Eimeria tenella and development of a decoquinate resistant strain. Avian Diseases 15, 342–5.CrossRefGoogle ScholarPubMed
McLoughlin, D. K. & Chute, M. B. (1973). Efficacy of nequinate against thirteen strains of E. tenella and the development of a nequinate resistant strain. Avian Diseases 17, 717–21.CrossRefGoogle Scholar
McLoughlin, D. K. & Gardiner, J. L. (1961). Drug resistance in Eimeria tenella. 1. The experimental development of a glycarbylamide resistant strain. Journal of Parasitology 47, 1001–6.CrossRefGoogle Scholar
McManus, E. C., Campbell, W. C. & Cuckler, A. C. (1968). Development of resistance to quinolone coccidiostats under field and laboratory conditions. Journal of Parasitology 54 1190–3.CrossRefGoogle Scholar
Millard, B. J. (1970). Observations on a drug resistant strain of Eimeria tenella. Research in Veterinary Science 11, 394–7.CrossRefGoogle ScholarPubMed
Ryley, J. F. & Betts, M. J. (1973). Chemotherapy of chicken coccidiosis. In Advances in Pharmacology and Chemotherapy, vol. 11 (ed. Garattini, S., Goldin, A., Hawking, F. and Kopin, I. J.), pp. 221–93. New York: London, Academic Press.Google Scholar