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Classification of plant trypanosomatids (Phytomonas spp.): parity between random-primer DNA typing and multilocus enzyme electrophoresis

Published online by Cambridge University Press:  01 October 1997


E. MULLER
Affiliation:
Laboratoire de Phytovirologie des Régions Chaudes, CIRAD-ORSTOM, BP 5035, 34032 Montpellier cedex 1, France
D. GARGANI
Affiliation:
Laboratoire de Phytovirologie des Régions Chaudes, CIRAD-ORSTOM, BP 5035, 34032 Montpellier cedex 1, France
A. L. BANULS
Affiliation:
Laboratoire de Génétique des Parasites et des Vecteurs, ORSTOM, BP 5045, 34032 Montpellier cedex 1, France
M. TIBAYRENC
Affiliation:
Laboratoire de Génétique des Parasites et des Vecteurs, ORSTOM, BP 5045, 34032 Montpellier cedex 1, France
M. DOLLET
Affiliation:
Laboratoire de Phytovirologie des Régions Chaudes, CIRAD-ORSTOM, BP 5035, 34032 Montpellier cedex 1, France

Abstract

The genetic polymorphism of 30 isolates of plant trypanosomatids colloquially referred to as plant trypanosomes was assayed by means of RAPD. The principle objectives of this study were to assess the discriminative power of RAPD analysis for studying plant trypanosomes and to determine whether the results obtained were comparable with those from a previous isoenzyme (MLEE) study. The principle groups of plant trypanosomes identified previously by isoenzyme analysis – intraphloemic trypanosomes, intralaticiferous trypanosomes and trypanosomes isolated from fruits – were also clearly separated by the RAPD technique. Moreover, the results showed a fair parity between MLEE and RAPD data (coefficient of correlation = 0·84) and the two techniques have comparable discriminative ability. Most of the separation revealed by the two techniques between the clusters was associated with major biological properties. However, the RAPD technique gave a more coherent separation than MLEE because the intraphloemic isolates, which were biologically similar in terms of their specific localization in the sieve tubes of the plant, were found to be in closer groups by the RAPD. For both techniques, the existence of the main clusters was correlated with the existence of synapomorphic characters, which could be used as powerful tools in taxonomy and epidemiology.


Type
Research Article
Copyright
1997 Cambridge University Press

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Classification of plant trypanosomatids (Phytomonas spp.): parity between random-primer DNA typing and multilocus enzyme electrophoresis
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Classification of plant trypanosomatids (Phytomonas spp.): parity between random-primer DNA typing and multilocus enzyme electrophoresis
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Classification of plant trypanosomatids (Phytomonas spp.): parity between random-primer DNA typing and multilocus enzyme electrophoresis
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