Skip to main content Accessibility help
×
Home

Trypanosoma rhipicephalis sp. nov. (Protozoa: Kinetoplastida) isolated from Rhipicephalus microplus (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil

  • Carolina R. Marotta (a1), Priscilla N. Dos Santos (a1), Matheus D. Cordeiro (a1), Paulo Cesar M. Matos (a1), Juliana Helena Da S. Barros (a2), Maria De Fatima Madeira (a2), Lesley Bell-Sakyi (a3) (a4) and Adivaldo H. Fonseca (a1)...

Abstract

Parasites of the genus Trypanosoma are unicellular flagellated microorganisms of the Trypanosomatidae. This study describes an isolate of the genus Trypanosoma naturally infecting Rhipicephalus microplus ticks, characterized through molecular, morphological and biological analysis. Trypanosome cultures, designated strain P1RJ, were obtained by isolation from R. microplus haemolymph in cultures of the tick cell line IDE8. After isolation, strain P1RJ grew well axenically in L15B medium at temperatures of 30, 32 and 34 °C. The new trypanosome remained stable in axenic culture over 14 passages in L15B at 30 °C and was successfully cryopreserved and resuscitated. Morphometric analysis was performed on randomly selected developmental forms. 18S rRNA and 24Sα rDNA sequence analyses confirmed that strain P1RJ is a new species of the genus Trypanosoma. The nucleotide sequences described were submitted to Genbank. Pathogenicity, involvement in vertebrate hosts, epidemiology, developmental cycle and transmission mechanisms of strain P1RJ are still unknown. Therefore, more studies will be necessary to determine life cycle aspects of this trypanosome, for which we propose the name Trypanosoma rhipicephalis sp. nov.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Trypanosoma rhipicephalis sp. nov. (Protozoa: Kinetoplastida) isolated from Rhipicephalus microplus (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Trypanosoma rhipicephalis sp. nov. (Protozoa: Kinetoplastida) isolated from Rhipicephalus microplus (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Trypanosoma rhipicephalis sp. nov. (Protozoa: Kinetoplastida) isolated from Rhipicephalus microplus (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil
      Available formats
      ×

Copyright

This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corresponding author

Author for correspondence: Adivaldo H. Fonseca, E-mail: adivaldofonseca@yahoo.com

References

Hide All
Andrade, S (1974) Caracterização de cepas do Trypanosoma cruzi isoladas no Recôncavo Baiano. Revista Patologia Tropical 3, 65121.
Barros, J, Toma, H and Madeira, M (2015) Molecular study of Trypanosoma caninum isolates based on different genetic markers. Parasitology Research 114, 777783.
Barros, JHS, Fonseca, TS, Macedo-Silva, RM, Côrte-Real, S, Toma, HK and Madeira, MdF (2014) Aflagellar epimastigote forms are found in axenic culture of Trypanosoma caninum . Acta Tropica 137, 147151.
Bastin, P, MacRae, TH, Francis, SB, Matthews, KR and Gull, K (1999) Flagellar morphogenesis: protein targeting and assembly in the paraflagellar rod of trypanosomes. Molecular and Cellular Biology 19, 81918200.
Brener, Z and Chiari, E (1965) Aspects of early growth of different Trypanosoma cruzi strains in culture medium. Journal of Parasitology 922926.
Chagas, C (1909) Nova tripanozomiaze humana: estudos sobre a morfolojia e o ciclo evolutivo do Schizotrypanum cruzi n. gen., n. sp., ajente etiolojico de nova entidade morbida do homem. Memórias do Instituto Oswaldo Cruz 1, 159218.
Elshafie, EI, Sani, RA, Hassan, L, Sharma, R, Bashir, A and Abubakar, IA (2013) Active infection and morphometric study of Trypanosoma evansi among horses in Peninsula Malaysia. Tropical Biomedical 30, 444450.
Gaido, AB, Mangold, AJ, Aguirre, DH and Guglielmone, AA (1989) Hallazgo de Trypanosomatidae en la garrapata Boophilus microplus en la Argentina. Revista de Medicina Veterinaria (B. Aires) 70, 134137.
García, L, Ortiz, S, Osorio, G, Torrico, MC, Torrico, F and Solari, A (2012) Phylogenetic analysis of Bolivian bat trypanosomes of the subgenus Schizotrypanum based on cytochrome B sequence and minicircle analyses. PLoS ONE 7, e36578.
Haag, J, O'HUigin, C and Overath, P (1998) The molecular phylogeny of trypanosomes: evidence for an early divergence of the Salivaria. Molecular and Biochemical Parasitology 91, 3749.
Hoare, C (1972) The Trypanosomes of Mammals. Oxford: Blackwell Scientific Publication, 749.
Latif, AA, Bakheit, MA, Mohamed, AEE and Zweygarth, E (2004) High infection rates of the tick Hyalomma anatolicum anatolicum with Trypanosoma theileri . Ondersteport Journal of Veterinary Research 71, 251256.
Li, JB, Gerdes, JM, Haycraft, CJ, Fan, Y, Teslovich, TM, May-Simera, H, Li, H, Blacque, OE, Li, L, Leitch, CC, Lewis, RA, Green, JS, Parfrey, PS, Leroux, MR, Davidson, W S, Beales, PL, Guay-Woodford, LM, Yoder, BK, Stormo, GD, Katsanis, N and Dutcher, SK (2004) Comparative genomics identifies a flagellar and basal body proteome that includes the BBS5 human disease gene. Cell 117, 541552.
Lima, L, Da Silva, FM, Neves, L, Attias, M, Takata, CS, Campaner, M, De Souza, W, Hamilton, PB and Teixeira, MM (2012) Evolutionary insights from bat trypanosomes: morphological, developmental and phylogenetic evidence of a new species, Trypanosoma (Schizotrypanum) erneyi sp. nov., in African bats closely related to Trypanosoma (Schizotrypanum) cruzi and allied species. Protist 163, 856872.
Madeira, MF, Sousa, MA, Barros, JHS, Figueiredo, FB, Fagundes, A, Schubach, A, De Paula, CC, Faissal, BNS, Fonseca, TS, Thoma, HK and Marzochi, MCA (2009) Trypanosoma caninum n. sp. (Protozoa: Kinetoplastida) isolated from intact skin of a domestic dog (Canis familiaris) captured in Rio de Janeiro, Brazil. Parasitology 136, 411423.
Madeira, MF, Almeida, ABPF, Barros, JHS, Oliveira, TSF, Sousa, VRF, Alves, AS, Miranda, LFC, Schubach, AO and Marzochi, MCA (2014) Trypanosoma caninum, a new parasite described in dogs in Brazil: aspects of natural infection. Journal of Parasitology 100, 231234.
Martins, JR, Leite, RC and Doyle, RL (2008) Tripanosomatides like Trypanosoma theileri in the cattle tick Boophilus microplus . Revista Brasileira de Parasitologia Veterinária 17, 113114.
Morzaria, SP, Latif, AA, Jongejan, F and Walker, AR (1986) Transmission of a Trypanosoma sp. to cattle by the tick Hyalomma anatolicum anatolicum . Veterinary Parasitology 19, 1321.
Munderloh, UG, Liu, Y, Wang, M, Chen, C and Kurtti, TJ (1994) Establishment, maintenance and description of cell lines from the tick Ixodes scapularis . Journal of Parasitology 80, 533543.
Nalbantoğlu, S and Karaer, Z (2008) Trypanosoma melophagium in blood cell culture. Ankara Universitesi Veteriner Fakultesi Dergisi 50, 11.
Ramírez, L, Dávila, A, Victório, A, Silva, R, Trajano, V and Jansen, A (1997) Measurements of Trypanosoma evansi from the Pantanal. Memórias do Instituto Oswaldo Cruz 92, 483484.
Rayat, C and Vasishta, R (2014) Wild rats as reservoir of Trypanosoma lewisi in Northwest India. Austin Journal of Pathology and Laboratory Medical 1, 3.
Ribeiro, MFB, Lima, JD and Guimarães, AM (1988) Ocorrência de Trypanosoma theileri, Laveran 1902, em Boophilus microplus, no Estado de Minas Gerais. Arquivos Brasileiros de Medicina Veterinaria e Zootecnia 40, 6568.
Schuster, FL and Sullivan, JJ (2002) Cultivation of clinically significant hemoflagellates. Clinical Microbiology Reviews 15, 374389.
Simo, G, Sobgwi, PF, Njitchouang, GR, Njiokou, F, Kuiate, JR, Cuny, G and Asonganyi, T (2013) Identification and genetic characterization of Trypanosoma congolense in domestic animals of Fontem in the South-West region of Cameroon. Infection, Genetics and Evolution 18, 6673.
Smith, A, Clark, P, Averis, S, Lymbery, AJ, Wayne, AF, Morris, KD and Thompson, RC (2008) Trypanosomes in a declining species of threatened Australian marsupial, the brush-tailed bettong Bettongia penicillata (Marsupialia: Potoroidae). Parasitology 135, 1329.
Souto, RP, Vargas, N and Zingales, B (1999) Trypanosoma rangeli: discrimination from Trypanosoma cruzi based on a variable domain from the large subunit ribosomal RNA gene. Experimental Parasitology 91, 306314.
Thekisoe, OMM, Honda, T, Fujita, H, Battsetseg, B, Hatta, T, Fujisaki, K, Sugimoto, C and Inoue, N (2007) A trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks in Kagoshima Prefecture, Japan. Parasitology 134, 967974.
Urdaneta-Morales, S (1983) Pleomorphism in trypomastigotes of Trypanosoma cruzi from blood and cell culture. Tropenmedizin und Parasitologie 34, 225228.
Villareal, MV, Mingala, CN and Rivera, WL (2013) Molecular characterization of Trypanosoma evansi isolates from water buffaloes (Bubalus bubalis) in the Philippines. Acta Parasitol 58, 612.
Wink, M (1979) Trypanosoma theileri: in vitro cultivation in tsetse fly and vertebrate cell culture systems. International Journal for Parasitology 9, 585589.
Ziccardi, M and Lourenço-de-Oliveira, R (1998) Morphological features of trypanosomes from squirrel monkeys from the Brazilian Amazon. Memórias do Instituto Oswaldo Cruz, 93, 301301.

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed