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Protein–phytate interactions in pig and poultry nutrition: a reappraisal

  • Peter H. Selle (a1), Aaron J. Cowieson (a1), Nathan P. Cowieson (a2) and V. Ravindran (a3)


Protein–phytate interactions are fundamental to the detrimental impact of phytate on protein/amino acid availability. The inclusion of exogenous phytase in pig and poultry diets degrades phytate to more innocuous esters and attenuates these negative influences. The objective of the present review is to reappraise the underlying mechanisms of these interactions and reassess their implications in pig and poultry nutrition. Protein digestion appears to be impeded by phytate in the following manner. Binary protein–phytate complexes are formed at pH levels less than the isoelectric point of proteins and complexed proteins are refractory to pepsin digestion. Once the protein isoelectric points are exceeded binary complexes dissociate; however, the isoelectric point of proteins in cereal grains may be sufficiently high to permit these complexes to persist in the small intestine. Ternary protein–phytate complexes are formed at pH levels above the isoelectric point of proteins where a cationic bridge links the protein and phytate moieties. The molecular weights of protein and polypeptides in small-intestinal digesta may be sufficient to allow phytate to bind nutritionally important amounts of protein in ternary complexes. Thus binary and ternary complexes may impede protein digestion and amino acid absorption in the small intestine. Alternatively, phytate may interact with protein indirectly. Myo-inositol hexaphosphate possesses six phosphate anionic moieties (HPO42–) that have strong kosmotropic effects and can stabilise proteins by interacting with the surrounding water medium. Phytate increases mucin secretions into the gut, which increases endogenous amino acid flows as the protein component of mucin remains largely undigested. Phytate promotes the transition of Na+ into the small-intestinal lumen and this suggests that phytate may interfere with glucose and amino acid absorption by compromising Na+-dependent transport systems and the activity of the Na pump (Na+-K+-ATPase). Starch digestion may be depressed by phytate interacting with proteins that are closely associated with starch in the endosperm of cereal grains. While elucidation is required, the impacts of dietary phytate and exogenous phytase on the site, rate and synchrony of glucose and amino acid intestinal uptakes may be of importance to efficient protein deposition. Somewhat paradoxically, the responses to phytase in the majority of amino acid digestibility assays in pigs and poultry are equivocal. A brief consideration of the probable reasons for these inconclusive outcomes is included in this reappraisal.

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1Hill, R & Tyler, C (1954) The reaction between protein and phytate. J Agric Sci 44, 324326.
2Daniel, TC, Sharpley, AN & Lemunyon, JL (1998) Agricultural phosphorus and eutrophication: a symposium overview. J Envir Qual 27, 251257.
3Lott, JNA, Ockenden, I, Raboy, V, et al. . (2000) Phytic acid and phosphorus in crop seed and fruits: a global estimate. Seed Sci Res 10, 1133.
4Wise, A (1983) Dietary factors determining the biological activity of phytates. Nutr AbstrRev Clin Nutr 53, 791806.
5Simons, PCM, Versteegh, HAJ, Jongbloed, AW, et al. . (1990) Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br J Nutr 64, 525540.
6Abelson, PH (1999) A potential phosphorus crisis. Science 283, 2015.
7Cordell, D, Drangert, J-O & White, S (2009) The story of phosphorus: global food security and food for thought. Global Envir Change 19, 292305.
8Bryden, WL, Selle, PH, Ravindran, V, et al. (2007) Phytate: an anti-nutritive factor in animal diets. In Poisonous Plants: Global Research and Solutions, pp. 279284 [Planter, KE, Wieregna, TL and Pfister, JA, editors]. Wallingford. Oxon: CABI Publishing.
9Selle, PH, Ravindran, V, Caldwell, RA, et al. . (2000) Phytate and phytase: consequences for protein utilisation. Nutr Res Rev 13, 255278.
10Costello, AJR, Glonek, T & Myers, TC (1976) 31P nuclear magnetic resonance-pH titrations on myo-inositol hexaphosphate. Carb Res 46, 159171.
11Jones, DB & Csonka, FA (1925) Proteins of the cottonseed. J Biol Chem 64, 673683.
12Mattson, S (1946) The cookability of yellow peas. A colloid-chemical and biochemical study. Acta Agric Suec 2, 185231.
13Bourdillon, J (1951) Crystalline bean seed protein in combination with phytic acid. J Biol Chem 189, 6572.
14Cosgrove, DJ (1966) The chemistry and biochemistry of inositol polyphosphates. Rev Pure Appl Chem 16, 209224.
15Cheryan, M (1980) Phytic acid interactions in food systems. CRC Crit Rev Food Sci Nutr 13, 297335.
16Anderson, PA (1985) Interactions between proteins and constituents that affect protein quality. In Digestibility and Amino Acid Availability in Cereals and Oilseeds, pp. 3145 [Finley, JW and Hopkins, DT, editors]. St Paul, MN: American Association of Cereal Chemists.
17Reddy, NR & Salunkhe, DK (1981) Interactions between phytate, protein, and minerals in whey fractions of black gram. J Food Sci 46, 564570.
18Barré, R & Nguyen-van-Hout, N (1965) Etude de la combinaison de l'acide phytique avec la serum-albumine humaine native, acetylée et des amine (Study of the combination of phytic acid with native, acetylated and deaminated human serum albumin). Bull Soc Chimie Biol 47, 13991417.
19Barré, R & Nguyen-van-Hout, N (1965) Etude de la combinaison de l'ovalbumine avec les acides phosphorique, β glycerophosphorique, pyrophosphorique et phytique (Study of the combination of ovalbumin with phosphoric, β-glycerophosphoric, pyrophosphoric and phytic acids). Bull Soc Chimie Biol 47, 14191431.
20Fontaine, TD, Pons, WA & Irving, GW (1946) Protein–phytate relationship in peanuts and cottonseed. J Biol Chem 164, 487507.
21Saio, K, Koyama, E & Watanabe, T (1967) Protein–calcium–phytic acid relationships in soybean. Part I. Effects of calcium and phosphorus on solubility characteristics of soybean meal protein. Agric Biol Chem 31, 11951200.
22Saio, K, Koyama, E & Watanabe, T (1968) Protein–calcium–phytic acid relationships in soybean. Part II. Effects of phytic acid on combination of calcium with soybean meal protein. Agric Biol Chem 32, 448452.
23De Rham, O & Jost, T (1979) Phytate–protein interactions in soybean extracts and low-phytate soy protein products. J Food Sci 44, 596600.
24Okubo, K, Myers, DV & Iacobucci, GA (1976) Binding of phytic acid to glycinin. Cereal Chem 53, 513524.
25Prattley, CA, Stanley, DW & van der Voort, FR (1982) Protein–phytate interactions in soybeans. II. Mechanism of protein–phytate binding as affected by calcium. J Food Biochem 6, 255271.
26Rutherfurd, SM, Edwards, ACE & Selle, PH (1997) Effect of phytase on lysine–rice pollard complexes. In Manipulating Pig Production, vol. VI, p. 248 [Cranwell, PD, editor]. Werribee, VIC: Australasian Pig Science Association.
27Rajendran, S & Prakash, V (1993) Kinetics and thermodynamics of the mechanism of interaction of sodium phytate with α-globulin. Biochemistry 32, 34743478.
28De Boland, AR, Garner, GB & O'Dell, BL (1975) Identification and properties of “phytate” in cereal grains and oilseed products. J Agric Food Chem 23, 11861189.
29Rivas, N, Dench, JE & Caygill, JC (1981) Nitrogen extractability of sesame (Sesamum indicum L.) seed and the preparation of two protein isolates. J Sci Food Agric 32, 565571.
30Prakash, V & Nandii, PK (1978) Isolation and characterization of α-globulin of sesame seed (Sesamum indicum L.). J Agric Food Chem 26, 320323.
31Champagne, ET (1988) Effects of pH on mineral–phytate, protein–mineral–phytate and mineral–fiber interactions. Possible consequences of atrophic gastritis on mineral bioavailability from high fiber foods. J Am Coll Nutr 7, 499508.
32Gillberg, L & Tornell, B (1976) Preparation of rapeseed protein isolates. Dissolution and precipitation behaviour of rapeseed proteins. J Food Sci 41, 10631069.
33O'Dell, BL & de Boland, A (1976) Complexation of phytate with proteins and cations in corn grain and oilseed meals. J Agric Food Chem 24, 804808.
34Champagne, ET, Rao, RM, Liuzzo, JA, et al. . (1985) The interactions of minerals, proteins, and phytic acid in rice bran. Cereal Chem 62, 231238.
35Ravindran, V, Cabahug, S, Ravindran, G, et al. . (1999) Influence of microbial phytase on apparent ileal amino acid digestibility in feedstuffs for broilers. Poult Sci 78, 699706.
36Ayres, JL, Branscomb, LL & Rogers, GM (1974) Processing of edible peanut flour and grits. J Am Oil Chem Assoc 51, 133136.
37Kies, AK, de Jonge, LH, Kemme, PA, et al. . (2006) Interaction between protein, phyate, and microbial phytase. In vitro studies. J Agric Food Chem 54, 17531758.
38Argos, P (1988) An investigation of protein subunit and domain interfaces. Protein Eng 2, 101113.
39Janin, J, Miller, S & Chothia, C (1988) Surface, subunit interfaces and interior of oligomeric proteins. J Molec Biol 204, 155164.
40Selle, PH, Cowieson, AJ & Ravindran, V (2009) Consequences of calcium interactions with phytate and phytase for poultry and pigs. Livest Sci 124, 126141.
41Lawlor, PG, Lynch, PB, Caffrey, PJ, et al. . (2005) Measurements of the acid-binding capacity of ingredients used in pig diets. Irish Vet J 58, 447452.
42Ravindran, V, Cabahug, S, Ravindran, G, et al. . (2000) Response of broiler chickens to microbial phytase supplementation as influenced by dietary phytic acid and non-phytate phosphorus levels. II. Effects on apparent metabolisable energy, nutrient digestibility and nutrient retention. Br Poult Sci 41, 193200.
43Scilingo, AA & Anon, MC (1996) Calorimetric study of soybean protein isolates: effect of calcium and thermal treatments. J Agric Food Chem 44, 37513756.
44Okubo, K, Myers, DV & Iacobucci, GA (1974) Binding of phytic acid to glycinin. Cereal Sci Today 19, 401, Abstr.
45Okubo, K, Iacobucci, GA & Myers, DV (1974) Effect of Ca2+ on phytate binding to glycinin. Cereal Sci Today 19, 401, Abstr.
46Nosworthy, N & Caldwell, RA (1987) The zinc(II) binding sites of soya bean glycinin. J Sci Food Agric 41, 5563.
47Nosworthy, N & Caldwell, RA (1988) The interaction of zinc(II) and phytic acid with soya bean glycinin. J Sci Food Agric 44, 143150.
48Jongeneel, CV, Bouvier, J & Bairoch, A (1989) A unique signature identifies a family of zinc-dependent metallopeptidases. Fed Eur Biochem Soc 2, 211214.
49Fraker, PJ, King, LE, Laakko, T, et al. . (2000) The dynamic link between the integrity of the immune system and zinc status. J Nutr 130, 1399S1406S.
50Zhou, JR, Fordyce, EJ, Raboy, V, et al. . (1992) Reduction of phytic acid in soybean products improves zinc bioavailability in rats. J Nutr 122, 24662473.
51Oberleas, D, Muhrer, ME & O'Dell, BL (1962) Effects of phytic acid on zinc availability and parakeratosis in swine. J Anim Sci 21, 5761.
52Cranwell, K & Liebman, M (1989) Effect of soybean fiber and phytate on serum zinc response. Nutr Res 9, 127132.
53McKinney, LL, Sollars, WF & Setzkorn, EA (1949) Studies on the preparation of soy bean protein free from phosphorus. J Biol Chem 178, 117132.
54Erdman, JW (1979) Oilseed phytates: nutritional implications. J Am Oil Chem Soc 56, 736741.
55Erdman, JW, Weingartner, KE, Mustakas, GC, et al. . (1980) Zinc and magnesium bioavailability from acid-precipitated and neutralized soybean protein products. J Food Sci 45, 11931199.
56Tzeng, Y-M, Diosady, LL & Rubin, LJ (1990) Production of canola protein materials by alkaline extraction, precipitation, and membrane processing. J Food Sci 55, 11471551.
57Deak, NA & Johnson, LA (2007) Fate of phytic acid in producing soy protein ingredients. J Am Oil Chem Soc 84, 369376.
58Champagne, ET, Fisher, MS & Hinojosa, O (1990) NMR and ESR studies of interactions among divalent cations, phytic acid, and N-acetyl-amino acids. J Inorg Biochem 38, 199215.
59Montagne, L, Crevieu-Gabriel, I, Toullec, R, et al. . (2003) Influence of dietary protein level and source on the course of protein digestion along the small intestine of the veal calf. J Dairy Sci 86, 934943.
60Montagne, L, Salgado, P, Toullec, R, et al. . (2002) Enzymes of the small intestine of the calf: effect of dietary protein source on the activities of some enzymes in the small intestinal mucosa and digesta. J Sci Food Agric 82, 17721779.
61Montagne, L, Toullec, R, Formal, M, et al. . (2000) Influence of dietary protein level and origin on the flow of mucin along the small intestine of the preruminant calf. J Dairy Sci 83, 28202828.
62Montagne, L, Toullec, R & Lalles, JP (2001) Intestinal digestion of dietary and endogenous proteins along the small intestine of calves fed soybean or potato. J Anim Sci 79, 27192730.
63Baldwin, RL (1996) How Hofmeister ion interactions affect protein stability. Biophys J 71, 20562063.
64Zhang, Y, Furyk, S, Bergbreiter, DE, et al. . (2005) Specific ion effects on the water solubility of macromolecules: PNIPAM and the Hofmeister series. J Am Chem Soc 127, 14051410.
65Cowieson, AJ & Cowieson, NP (2011) Phytate and the thermodynamics of water. Proc Aust Poult Sci Symp 22, 2225.
66Thompson, AR (1955) Amino acid sequence in lysozyme. Biochem J 60, 507515.
67Shafey, TM, McDonald, MW & Dingle, JG (1991) Effects of dietary Ca and available phosphorus concentration on digesta pH and on the availability of calcium, iron, magnesium, and zinc from the intestinal contents of meat chickens. Br Poult Sci 32, 185194.
68Engberg, RM, Hedemann, MS & Jensen, BB (2002) The influence of grinding and pelleting on the microbial composition and activity in the digestive tract of broiler chickens. Br Poult Sci 44, 569579.
69Li, Z, Yi, G, Yin, J, et al. . (2008) Effects of organic acids on growth performance, gastrointestinal pH, intestinal microbial populations and immune responses of weaned pigs. Asian-Austr J Anim Sci 21, 252261.
70Monaghan-Watts, B (1937) Whipping ability of soybean proteins. Ind Eng Chem 29, 10091011.
71Csonka, FA, Murphy, JC & Jones, DB (1926) The iso-electric points of various proteins. J Am Chem Soc 48, 763768.
72Rutherfurd, SM, Chung, TK & Moughan, PJ (2002) The effect of microbial phytase on ileal phosphorus and amino acid digestibility in the broiler chicken. Br Poult Sci 43, 598606.
73Camus, MC & Laporte, JC (1976) Inhibition de la protéolyse pesique par le blé. Rôle de l'acide phytique des issues (Inhibition of pepsin proteolysis by wheat. Role of phytic acid in the outcome). Annal Biol Anim Biochim Biophys 16, 719729.
74Inagawa, J, Kiyosawa, I & Nagasawa, T (1987) Effects of phytic acid on the digestion of casein and soybean protein with trypsin, pancreatin and pepsin. Nippon Eiyo Shokuryo Gakkaishi 40, 367373.
75Kanaya, K, Yasumoto, K & Mitsuda, H (1976) Pepsin inhibition by phytate contained in rice bran. Eiyo To Shokuryo 29, 341346.
76Knuckles, BE, Kuzmicky, DD, Gumbmann, MR, et al. . (1989) Effect of myo-inositol phosphate esters on in vitro and in vivo digestion of protein. J Food Sci 54, 13481350.
77Vaintraub, IA & Bulmaga, VP (1991) Effect of phytate on the in vitro activity of digestive proteinases. J Agric Food Chem 39, 859861.
78Krehbiel, CR & Matthews, JC (2003) Absorption of amino acids and peptides. In Amino Acids in Animal Nutrition, 2nd ed., pp. 4170 [D'Mello, JPF, editor]. Wallingford, Oxon: CAB International.
79Mitjavila, S, de Saint Blanquat, G & Derache, R (1973) Effect de l'acide tannique sur la sécrétion gastrique chez le rat (Effect of tannic acid on gastric sectretion in the rat). Nutr Metab 15, 163170.
80Decuypere, JA, Knockaert, P & Henderickx, HK (1981) In vitro and in vivo protein digestion in pigs fed diets containing soybean protein isolates with different physical properties. J Anim Sci 53, 12971308.
81Allen, A & Flemström, G (2005) Gastroduodenal mucus bicarbonate barrier: protection against acid and pepsin. Am J Physiol Cell Physiol 288, C1C19.
82Munster, DJ, Bagshaw, PF & Wilson, JG (1987) Peptic erosion of gastric mucus in the rat. Comp Biochem Physiol A Comp Physiol 87, 509513.
83Cowieson, AJ, Acamovic, T & Bedford, MR (2004) The effects of phytase and phytic acid on the loss of endogenous amino acids and minerals from broiler chickens. Br Poult Sci 45, 101108.
84Onyango, EM, Asem, EK & Adeola, O (2009) Phytic acid increases mucin and endogenous amino acid losses from the gastrointestinal tract of chickens. Br J Nutr 101, 836842.
85Lien, KA, Sauer, WC & He, JM (2001) Dietary influences on the secretion into and degradation of mucin in the digestive tract of monogastric animals and humans. J Anim Feed Sci 10, 223245.
86Satchithanandam, S, Jahangeer, S, Cassidy, MM, et al. . (1989) Quantitative effects of wheat bran feeding on rat small intestinal mucin. FASEB J 3, A1066.
87Selle, PH, Walker, AR & Bryden, WL (2003) Total and phytate-phosphorus contents and phytase activity of Australian-sourced feed ingredients for pigs and poultry. Aust J Exp Agric 45, 475479.
88Onyango, E, Madsen, C & Gendler, S (2008) Inositol hexaphosphate alters mucin expression in the intestinal mucosa of mice. FASEB J 22, 1189.8.
89Lien, KA, Sauer, WC & Fenton, M (1997) Mucin output in ileal digesta of pigs fed a protein-free diet. Z Ernährungswiss 36, 182190.
90Cowieson, AJ & Ravindran, V (2007) Effect of phytic acid and microbial phytase on the flow and amino acid composition of endogenous protein at the terminal ileum of growing broiler chickens. Br J Nutr 98, 745752.
91Cowieson, AJ, Ravindran, V & Selle, PH (2008) Influence of dietary phytic acid and source of microbial phytase on ileal endogenous amino acid flows in broiler chickens. Poult Sci 87, 22872299.
92Cowieson, AJ, Bedford, MR, Selle, PH, et al. . (2009) Phytate and microbial phytase: implications for endogenous nitrogen losses and nutrient availability. Worlds Poult Sci J 65, 401417.
93Montagne, L, Piel, C & Lalles, JP (2004) Effect of diet on mucin kinetics and composition: nutrition and health implications. Nutr Rev 62, 104114.
94Bohak, Z (1969) Purification and characterization of chicken pepsinogen and chicken pepsin. J Biol Chem 244, 46384648.
95Desphande, SS & Cheryan, M (1984) Effects of phytic acid divalent cations and their interactions on α-amylase activity. J Food Sci 49, 516519.
96Sultan, A, Gan, CY, Li, X, et al. . (2011) Dietary enzyme combinations improve sorghum ileal protein and starch digestibility during the broiler starter phase. Proc Aust Poult Sci Symp 22, 82.
97Sultan, A, Gan, CY, Li, X, et al. . (2011) Dietary enzymes modulate sorghum starch digestion kinetics in broilers. Proc Aust Poult Sci Symp 22, 83.
98Nyman, ME & Björk, IM (1989) In vivo effects of phytic acid and polyphenols on the bioavailability of polysaccharides and other nutrients. J Food Sci 54, 13321335.
99Zaefarian, F, Romero, LF & Ravindran, V (2011) Effects of microbial phytase on nutrient digestibility and energy utilisation in broiler starters fed phosphorous-adequate diets. Proc Aust Poult Sci Symp 22, 8891.
100Liu, N, Ru, Y, Wang, J & Xu, T (2010) Effect of dietary sodium phytate and microbial phytase on the lipase activity and lipid metabolism of broiler chickens. Br J Nutr 103, 862868.
101Ravindran, V, Morel, PCH, Partridge, GG, et al. . (2006) Influence of an E. coli-derived phytase on nutrient utilization in broiler starters fed diets containing varying concentrations of phytic acid. Poult Sci 85, 8289.
102Selle, PH, Partridge, GG & Ravindran, V (2009) Beneficial effects of xylanase and/or phytase inclusions on ileal amino acid digestibility energy utilisation mineral retention and growth performance in wheat-based broiler diets. Anim Feed Sci Technol 153, 303313.
103Rickard, SE & Thompson, LU (1997) Interactions and biological effects of phytic acid. In Antinutrients and Phytochemicals in Food, pp. 294312 [Shahidi, F, editor]. Washington, DC: American Chemical Society.
104Blennow, A, Bay-Smidt, AM, Olsen, CE, et al. . (2000) The distribution of covalently bound phosphate in the starch granule in relation to starch crystallinity. Int J Biol Macromol 27, 211218.
105Thompson, LU (1988) Antinutrients and blood glucose. Food Technol 42, 123131.
106Thompson, LU (1988) Phytic acid: a factor influencing starch digestibility and blood glucose response. In Phytic Acid: Chemistry and Applications, pp. 173194 [Graf, E, editor]. Minneapolis, MN: Pilatus Press.
107Rooney, LW & Pflugfelder, RL (1986) Factors affecting starch digestibility with special emphasis on sorghum and corn. J Anim Sci 63, 16071623.
108Takeuchi, I (1969) Interaction between protein and starch. Cereal Chem 46, 570579.
109Anderson, IA, Levine, AS & Levitt, MD (1981) Incomplete absorption of the carbohydrate in all-purpose wheat flour. N Engl J Med 304, 891892.
110Thorne, MJ, Thompson, LU & Jenkins, DJA (1983) Factors affecting starch digestibility and the glycemic response with special reference to legumes. Am J Clin Nutr 38, 481488.
111Jenkins, DJA, Thorne, MJ & Wolever, TMS (1987) The effect of starch–protein interaction in wheat on the glycemic response and rate of in vitro digestion. Am J Clin Nutr 45, 946951.
112Baldwin, PM (2001) Starch granule-associated proteins and polypeptides: a review. Starch/Starke 53, 475503.
113Ryan, KJ & Brewer, MS (2007) In situ examination of starch-granule–soy protein and wheat protein interactions. Food Chem 104, 619629.
114Eliasson, A-C & Tjerneld, E (1990) Adsorption of wheat proteins and wheat starch granules. Cereal Chem 67, 366372.
115Camden, BJ, Morel, PCH, Thomas, DV, et al. . (2001) Effectiveness of exogenous microbial phytase in improving the bioavailabilities of phosphorus and other nutrients in maize–soya-bean meal diets for broilers. Anim Sci 73, 289297.
116Selle, PH, Cadogan, DJ, Li, X, et al. . (2010) Implications of sorghum in broiler chicken nutrition. Anim Feed Sci Technol 156, 5774.
117Ketaren, PP, Batterham, ES, Dettmann, EB, et al. . (1993) Phosphorus studies in pigs. 3. Effect of phytase supplementation on the digestibility and availability of phosphorus in soya-bean meal for grower pigs. Br J Nutr 70, 289311.
118Selle, PH & Ravindran, V (2007) Microbial phytase in poultry nutrition. Anim Feed Sci Technol 135, 141.
119Selle, PH & Ravindran, V (2008) Phytate-degrading enzymes in pig nutrition. Livest Sci 113, 99122.
120Selle, PH, Ravindran, V, Bryden, WL, et al. . (2006) Influence of dietary phytate and exogenous phytase on amino acid digestibility in poultry: a review. J Poult Sci 43, 89103.
121Dilger, RN, Onyango, EM, Sands, JS, et al. . (2004) Evaluation of microbial phytase in broiler diets. Poult Sci 83, 962970.
122Onyango, EM, Bedford, MR & Adeola, O (2005) Efficacy of an evolved Escherichia coli phytase in diets for broiler chicks. Poult Sci 84, 248255.
123Ravindran, V, Selle, PH & Bryden, WL (1999) Effects of phytase supplementation, individually and in combination, with glycanase on the nutritive value of wheat and barley. Poult Sci 78, 15881595.
124Ravindran, V, Selle, PH, Ravindran, G, et al. . (2001) Microbial phytase improves performance, apparent metabolizable energy and ileal amino acid digestibility of broilers fed a lysine-deficient diet. Poult Sci 80, 338344.
125Ravindran, V, Cowieson, AJ & Selle, PH (2008) Influence of dietary electrolyte balance and microbial phytase on growth performance, nutrient utilization and excreta quality of broiler chickens. Poult Sci 87, 677688.
126Rutherfurd, SM, Chung, TK, Morel, PCH, et al. . (2004) Effect of microbial phytase on ileal digestibility of phytate phosphorus, total phosphorus, and amino acids in a low-phosphorus diet for broilers. Poult Sci 83, 6168.
127Selle, PH, Ravindran, V, Ravindran, G, et al. . (2003) Influence of phytase and xylanase supplementation on growth performance and nutrient utilisation of broilers offered wheat based diets. Asian-Austral J Anim Sci 16, 394402.
128Watson, BC, Matthews, JO, Southern, LL, et al. . (2006) The effects of phytase on growth performance and intestinal transit time of broilers fed nutritionally adequate diets and diets deficient in calcium and phosphorus. Poult Sci 85, 493497.
129Sebastian, S, Touchburn, SP, Chavez, ER, et al. . (1997) Apparent digestibility of protein and amino acids in broiler chickens fed a corn–soybean diet supplemented with microbial phytase. Poult Sci 76, 17601769.
130Yi, Z, Kornegay, ET & Denbow, DM (1996) Effect of microbial phytase on nitrogen and amino acid digestibility and nitrogen retention of turkey poults fed corn–soybean meal diets. Poult Sci 75, 979990.
131Officer, DI & Batterham, ES (1992) Enzyme supplementation of LinolaTM meal for grower pigs. Proc Aust Soc Anim Prod 19, 288.
132Officer, DI & Batterham, ES (1992) Enzyme supplementation of LinolaTM meal. In Fourth Biennial Pig Industry Seminar, p. 56. Wollongbar, NSW:Wollongbar Agricultural Institute.
133Kornegay, ET, Radcliffe, JS & Zhang, Z (1998) Influence of phytase and diet composition on phosphorus and amino acid digestibilities, and phosphorus and nitrogen excretion in swine. In BASF Technical Symposium: preceding Carolina Swine Nutrition Conference, pp. 125155. Mount Olive, NJ: BASF Corporation.
134Zeng, ZK, Piao, XS, Wang, D, et al. . (2011) Effect of microbial phytase on performance, nutrient absorption and excretion in weaned pigs and apparent ileal nutrient digestibility in growing pigs. Asian-Aust J Anim Sci 24, 11641172.
135Kiarie, E, Owusu-Asiedu, A, Simmins, PH, et al. . (2010) Influence of phytase and carbohydrase enzymes on apparent ileal nutrient and standardized ileal amino acid digestibility in growing pigs fed wheat and barley-based diets. Livest Sci 134, 8587.
136Nitrayova, S, Patras, P, Brestensky, M, et al. . (2009) Effect of microbial phytase and diet fermentation on ileal and total tract digestibility of nutrients and energy in growing pigs. Czech J Anim Sci 54, 163174.
137Sacrane, A, Iji, PA, Millelsen, LL, et al. . (2007) Occurrence of reverse peristalsis in broiler chickens. Proc Aust Poult Sci Symp 19, 161164.
138Rowan, AM, Moughan, PJ & Wilson, MN (1992) The flows of deoxyribonucliec acid and diaminopimelic acid and the digestibility of dietary fibre components at the terminal ileum, as indicators of microbial activity in the upper digestive tract of ileostomised pigs. Anim Feed Sci Technol 36, 129141.
139Easter, RA & Tanksley, TD (1973) A technique for re-entrant ileocecal cannulation of swine. J Anim Sci 36, 10991103.
140Miner-Williams, W, Moughan, PJ & Fuller, MF (2009) Endogenous components of digesta protein from the terminal ilelum of pigs fed a casein-based diet. J Agric Food Chem 57, 20722078.
141Brand, TS, Badenhorst, HA, Siebrits, FK, et al. . (1990) The use of pigs both intact and with ileo-rectal anastomosis to estimate the apparent and true digestibility of amino acids in untreated, heat-treated and thermal-ammoniated high-tannin grain sorghum. S Afr J Anim Sci 20, 223228.
142Newkirk, RW & Classen, HL (2001) The non-mineral nutritional impact of phytate in canola meal for broiler chicks. Anim Feed Sci Technol 91, 115128.
143Selle, PH, Ravindran, V, Ravindran, G, et al. . (2007) Effects of dietary lysine and microbial phytase on growth performance and nutrient utilisation of broiler chickens. Asian-Austral J Anim Sci 20, 11001107.
144Torras-Llort, M, Soriano-García, JF, Ferrer, R, et al. . (1998) Effect of a lysine-enriched diet on l-lysine transport by the brush-border membrane of the chicken jejunum. Am J Physiol 274, R69R75.
145Vickery, HB & White, A (1933) The basic amino acids of casein. J Biol Chem 103, 413415.
146Vioque, J, Sanchez-Vioque, R, Clemente, A, et al. . (1999) Production and characterization of an extensive rapeseed protein hydrolysate. J Am Oil Chem Soc 76, 819823.
147Bau, HM, Mohtadi-Ni, DJ, Mejean, L, et al. . (1983) Preparation of colorless sunflower protein products: effect of processing on physicochemical and nutritional properties. J Am Oil Chem Soc 60, 11411148.


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Protein–phytate interactions in pig and poultry nutrition: a reappraisal

  • Peter H. Selle (a1), Aaron J. Cowieson (a1), Nathan P. Cowieson (a2) and V. Ravindran (a3)


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