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Molecular typing of Spanish species of Amanita by restriction analysis of the ITS region of the DNA

Published online by Cambridge University Press:  24 October 2002

Vicente GONZÁLEZ ,
Francisco ARENAL ,
Gonzalo PLATAS ,
Fernando ESTEVE-RAVENTÓS  and
Fernando PELÁEZ
Vicente GONZÁLEZ
Affiliation:
Centro de Investigación Básica. Merck, Sharp & Dohme de España, S.A., Calle Josefa Valcárcel 38, E-28027 Madrid, Spain. Current address: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología (CSIC-UAM), campus Cantoblanco, Universidad Autónoma de Madrid, E-28049 Madrid, Spain.
Francisco ARENAL
Affiliation:
Centro de Investigación Básica. Merck, Sharp & Dohme de España, S.A., Calle Josefa Valcárcel 38, E-28027 Madrid, Spain. Current address: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología (CSIC-UAM), campus Cantoblanco, Universidad Autónoma de Madrid, E-28049 Madrid, Spain.
Gonzalo PLATAS
Affiliation:
Centro de Investigación Básica. Merck, Sharp & Dohme de España, S.A., Calle Josefa Valcárcel 38, E-28027 Madrid, Spain.
Fernando ESTEVE-RAVENTÓS
Affiliation:
Department of Plant Biology, Alcalá University, E-28871 Alcalá de Henares, Spain.
Fernando PELÁEZ
Affiliation:
Centro de Investigación Básica. Merck, Sharp & Dohme de España, S.A., Calle Josefa Valcárcel 38, E-28027 Madrid, Spain.
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Abstract

The genetic relatedness among 29 collections belonging to 20 of the most common species of genus Amanita in the Iberian Peninsula have been studied by means of restriction analysis of the amplified ITS1-5.8S-ITS2 region of rDNA. Sixty restriction fragments were considered for the analysis. The main conclusion is that this technique could be useful to identify and characterize isolates from Amanita species. Although ARDRA did not show an appropriate level of discrimination to unambiguously infer phylogenetic relationships at or below the section level, some general trends could be outlined when the different haplotypes generated were compared by means of neighbour joining analysis. Thus, members from the same sections were frequently grouped together.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 8 , August 2002 , pp. 903 - 910
Copyright
© The British Mycological Society 2002

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