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Genetic differentiation of Phoma exigua varieties by means of AFLP fingerprints

Published online by Cambridge University Press:  01 August 2002

Edwin C. A. ABELN ,
Annelein M. STAX ,
Johannes de GRUYTER  and
Hubert A. van der AA
Edwin C. A. ABELN
Affiliation:
Centraalbureau voor Schimmelcultures, P.O. Box 85167, 3508 AD Utrecht, The Netherlands. E-mail: abeln@cbs.knaw.nl
Annelein M. STAX
Affiliation:
Centraalbureau voor Schimmelcultures, P.O. Box 85167, 3508 AD Utrecht, The Netherlands. E-mail: abeln@cbs.knaw.nl
Johannes de GRUYTER
Affiliation:
Plant Protection Service, P.O. Box 9102, 6700 HC Wageningen, The Netherlands.
Hubert A. van der AA
Affiliation:
Centraalbureau voor Schimmelcultures, P.O. Box 85167, 3508 AD Utrecht, The Netherlands. E-mail: abeln@cbs.knaw.nl
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Abstract

Several varieties have been distinguished within the phytopathogenic pycnidial fungus Phoma exigua on the basis of cultural characteristics. These varieties have been stated to correlate with differences in ability to attack different host plants. Molecular discrimination of the varieties was investigated using amplified fragment length polymorphism (AFLP) and sequence analysis of the ITS region of the rDNA cluster on a set of 43 strains and 7 outgroup strains. The ITS sequences of the 43 different P. exigua strains were highly similar and revealed no subgroups within P. exigua while the AFLP fingerprint patterns of two primer combinations showed clear clustering of most varieties.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 4 , April 2002 , pp. 419 - 427
Copyright
© The British Mycological Society 2002

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