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Detection of genetic variation in Alternaria brassicicola using AFLP fingerprinting

Published online by Cambridge University Press:  01 August 2002

Clive H. BOCK
Affiliation:
Centre for Plant Biodiversity Research, CSIRO-Plant Industry, GPO 1600, Canberra, ACT 2601, Australia. Current address: Division of Plant Pathogen Interactions, IACR-Rothamsted, Harpenden AL5 2JQ, Herts, UK.
Peter H. THRALL
Affiliation:
Centre for Plant Biodiversity Research, CSIRO-Plant Industry, GPO 1600, Canberra, ACT 2601, Australia.
Curt L. BRUBAKER
Affiliation:
Centre for Plant Biodiversity Research, CSIRO-Plant Industry, GPO 1600, Canberra, ACT 2601, Australia.
Jeremy J. BURDON
Affiliation:
Centre for Plant Biodiversity Research, CSIRO-Plant Industry, GPO 1600, Canberra, ACT 2601, Australia.
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Abstract

Genetic variation within and between eighteen isolates of Alternaria brassicicola, five isolates of A. alternata, and a single isolate of Rhynchosporium secalis was compared using Amplified Fragment Length Polymorphisms (AFLPs). The AFLPs consistently distinguished between the three species. AFLPs thus provide a reliable tool for identifying A. brassicicola. The analysis of eighteen isolates of A. brassicicola from different sites also indicated at least moderate levels of genetic diversity within A. brassicicola along the New South Wales coast. Of 43–66 markers scored per primer combination, 16·7–27·9% were polymorphic. On the basis of an unweighted paired group method of arithmetic averages analysis using data from four primer combinations, most isolates were identified as separate genotypes. However, multiple isolates from particular locations tended to cluster together, implying the potential for population structure, and there was a significant positive correlation between Nei's measure of genetic distance and physical distance (r = 0·5486, P<0·001). Despite the absence of an identified sexual stage, A. brassicicola would appear to have a means for generating and maintaining significant variation.

Type
Research Article
Copyright
© The British Mycological Society 2002

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