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A Simple Stage Heater / Cooler For Light Microscopy

Published online by Cambridge University Press:  14 March 2018

Anthony G. Moss
Anthony G. Moss*
Affiliation:
Zoology and Wildlife Sciences, Auburn University

Extract

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Microscopists frequently find that they need to regulate temperature during the course of their experiments. This is especially true for cell biologists working with living cells, Excellent commercial equipment does exist to control the stage temperature of light microscopes, and regulation by such devices is tightly controlled; ± 0.1°C is routinely reported. However, such equipment is often quite expensive and may not provide the investigator with the certainty that the experiment is being performed at exactly the temperature reported by the readout of the thermoelectric control, since the temperature sensor is often at a distance from the actual site of observation. The temperature also varies considerably from the stage heater/cooler to the critical, observed portion of the slide.

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 8 , October 1998 , pp. 16 - 19
Copyright
Copyright © Microscopy Society of America 1998

References

Literature Cited

Chabala, L. D., Sheridan, R. E., Hodge, D.C., Power, J. N., and Walsh, M. P. 1965, A microscope stage temperature controller for the study of whole-cell or single-channel currents. Pfluger's Arch. 404:374–377.Google Scholar
Datyner, N. B., Gintant, G A, and Cohen, I. S., 1985. Versatile temperature-controlled tissue bath for studies of isolated cells using an inverted microscope, Pfluger's Arch. 403:318–323.Google Scholar
Inoue, S., Fuseler, J., Salman, E. D., and Ellis, G. W., 1975. Functional organization of mitotic microtubules. Physical chemistry of the in vivo equilibrium system, Biophys. J. 15:725–744.Google Scholar
Inoue, S. and Spring, K. R., 1998, Video Microscopy. The Fundamentals, 2nd ed. Plenum Publishing Corporation, New York, NY. 741 pp.Google Scholar
Horowitz, P. and Hill, W., 1980. The Art of Electronics: Cambridge University Press, New York, NY. 716 pp,Google Scholar
McKenna, N.M., and Wang, Y-L., 1989. Culturing cells on the microscope stage. Math. Cel Bid. 29:195–205.Google ScholarPubMed
Moss, A. G., Gatti, J-L, and Witman, G. B., 1992. The motile β/1C1 subunii of sea urchin sperm outer arm dynein does not form a tight rigor bond, J. Cell Biol. 118 (5)1177–1188.Google Scholar