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Reflected/Transmitted Nomarsky DIC Lighting

Published online by Cambridge University Press:  14 March 2018

Ely Silk*
Affiliation:
Cryolume Scientific Company

Extract

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What could be better than reflected or transmitted Nomarski differential interference contrast? Why, combining the best features of both and for very little cost. My intended use of the technique was for Nomarski reflection DIC microscopy. It will work, of course, with other types of reflection microscopy.

What this embarrassingly simple artifice accomplishes is to simultaneously add transmission capabilities to reflection observations with the result being improved viewing of delicate details, And, yes, because of the reflective front surface layer, the observer can study details on the bottom side of the specimen which is usually hidden from view. This requires focusing through the specimen and below the point of normal focus.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 1998