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Quantification of Virus Suspensions by Direct Particle Counting

Published online by Cambridge University Press:  14 March 2018

Paul R. Hazelton
Paul R. Hazelton*
Affiliation:
University of Manitoba, Winnipeg, Manitoba, Canada
*
paul_hazelton@umanitoba.ca

Extract

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In science, there are occasions when it is of value to know the number and distribution of different types of particles present in a solution. Calculation of these values is simplified if you can uniformly distribute the number of particles in a known volume over an established surface area. A number of procedures have been proposed. The best which we have used is direct centrifugation of particles to formvar/carbon-formvar coated electron microscope grids in an EM-90 rotor for the Beckman Airfuge®. This instrument makes use of the ability to pellet all material in a suspension using established centrifugation theory.

Type
Research Article
Information
Microscopy Today , Volume 14 , Issue 5 , September 2006 , pp. 40 - 45
Copyright
Copyright © Microscopy Society of America 2006

References

References:

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