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Processing Cochlea For Paraffin Sectioning And SEM

Published online by Cambridge University Press:  14 March 2018

Donna C. Montague
Donna C. Montague*
Affiliation:
University of Arkansas for Medical Sciences

Extract

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We have successfully sectioned decalcified paraffin embedded “inner ears” from mice, rats and humans. We have also prepared guinea pig cochlea for SEM, So, briefly:

Paraffin:

  1. 1) Immediately after dissection, place the cochlea in 10% neutral buffered formalin (NSF) and let fix with gentle agitation 24 hours per 2 mm thickness at room temperature.

  2. 2) Decalcify by immersing in 5% formic acid at least 4X specimen volume.

  3. 3) Gently agitate at room temperature and change at least once per 24 hours. Check for completeness of decalcification by decanting 4 mLs of “spent” formic acid into a clean, clear test tube, Add 1 mL of 5% ammonium oxaiate in distilled water. Let stand undisturbed for 15 to 20 minutes, White precipitate of calcium oxaiate indicates that the sample is not completely decalcified, Change formic acid at least once per 24 hours until the spent formic tests clear.

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 5 , July 1998 , pp. 28 - 29
Copyright
Copyright © Microscopy Society of America 1998

References

References:

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