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Probing Ribosomal RNA By Electron Spectroscopic Imaging and Three-Dimensional Reconstruction

Published online by Cambridge University Press:  14 March 2018

Daniel R. Beniac ,
Gregory J. Czarnota ,
Brenda L. Rutherford ,
F. Peter Ottensmeyer  and
George Harauz
Daniel R. Beniac
Affiliation:
Department of Molecular Biology and Genetics, and Biophysics Interdisciplinary Group, University of Guelph
Gregory J. Czarnota
Affiliation:
Molecular and Structural Biology, Ontario Cancer Institute, and Department of Medical Biophysics, University of Toronto
Brenda L. Rutherford
Affiliation:
Molecular and Structural Biology, Ontario Cancer Institute, and Department of Medical Biophysics, University of Toronto
F. Peter Ottensmeyer
Affiliation:
Molecular and Structural Biology, Ontario Cancer Institute, and Department of Medical Biophysics, University of Toronto
George Harauz
Affiliation:
Department of Molecular Biology and Genetics, and Biophysics Interdisciplinary Group, University of Guelph

Extract

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The ribosome is the protein synthetic machinery in the cell. Knowledge of the structures of ribosomal RNA (rRNA) macromolecules in situ is essential to understanding their roles in ribosome mediated protein synthesis. We are using a microanalytical technique that identifies and maps elements directly, electron spectroscopic imaging, to determine the rRNA phosphorus distributions within Escherichia coli ribosomal subunits, and to combine the two-dimensional maps into a three-dimensional elemental distribution by iterative quaternion-assisted angular reconstitution of ribosomal particles at random orientations.

Type
Research Article
Information
Microscopy Today , Volume 5 , Issue 1 , January 1997 , pp. 10 - 11
Copyright
Copyright © Microscopy Society of America 1997

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