No CrossRef data available.
Article contents
Pretreating Epoxy Thin Sections With Sodium Periodate Prior To Immunostaining
Published online by Cambridge University Press: 14 March 2018
Extract
Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Pretreatment of epoxy thin sections with strong oxidizing agents such as hydrogen peroxide, sodium methoxide, and sodium m-metaperiodate facilities the location of antigens with immunostaining procedures. Etching, or pretreatment, of sections unmasks antigenic sites on glutaraldehyde fixed and postosmicated tissue, partially removes osmium bonds, temporarily decreases the hydrophobicity of the epoxy surface layer of the section, reduces the electron density of the tissue and increases resistance to heavy rnetai poststaining.
- Type
- Research Article
- Information
- Copyright
- Copyright © Microscopy Society of America 1997
References
1. Bendyan, M. and Zollinger, M., Ultrastructural Localization of AnSigenic Sites on Osmium-fixed Tissues Applying the Protein A-Gold Technique. J. Histochem and Cylocnem., Vol.31, No.1, p.101, 1983.Google Scholar
2. Newman, G. and Hobol, J., Resin Microscopy and On-Section Imrnunocytochemistiy Springer Verlag, Berlin 1993. p.131.CrossRefGoogle Scholar
3. Causton, B., Does the Embedding Chemistry Interact with Tissue? Science of Biological Specimen Preparation for Microscopy and Microanalysis, in Scanning Electron Microscopy. Inc. Illinois, 1985, p.209.Google Scholar
4. Phend, K., Rustloni, A. and Weiriber, R., An Osmium-free Method of Epon Embedment That Preserves both UUrastmcture and Antigenicjty for Post-embedding Immunoctyocriemistry. J. HistochemandCytochem,. Vol 43. No. 3, p.283, 1995.Google Scholar
You have
Access