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Loss of Antigenicity on Stored Paraffin Sections

Published online by Cambridge University Press:  14 March 2018

M A. Hayat
M A. Hayat*
Affiliation:
Kean University, Union, NJ

Extract

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Sections of formalin-fixed and paraffin-embedded tissues are commonly used to detect antigens of diagnostic, therapeutic, or prognostic importance in patients with many types of cancers. Sections (- 5 μm thick) cut from paraffin-embedded tissues are mounted on glass slides usually one or two days before immunostaining. In some cases, paraffin blocks are no longer available, and so immunohistochemistry must be performed on unstained slides that have been prepared sometime ago. it is not uncommon in both clinical and research laboratories to store unstained paraffin-embedded sections on glass slides at room temperature. Also, research laboratories that serve as reference centers for multi-institutional studies store slides for future use in order to rapidly return the tissue blocks to the originating institution. Such institutions want tissue blocks returned to them as soon as possible, as this may have medicolegal implications.

Storage of sections also becomes necessary when one marker study is proceeding and future marker studies need to be followed.

Type
Research Article
Information
Microscopy Today , Volume 8 , Issue 8 , October 2000 , pp. 30 - 32
Copyright
Copyright © Microscopy Society of America 2000

References

Bertheau, P., Cazals-Hatem, D., Meignin, V., de Roquancourt, A., Verola, O, Lesourd, A., Sene, t , Brocheriou, C, and Janin, A. 1998. Variability of immunohistochemical reactivity on stored paraffin slides. J. Clin. Pathol. 51:370.Google Scholar
Dwork, A.J., Liu, D., Kaufman, M.A., and Prohovnik, I. 1998. Archival, formalun-fixed tissue: its use in the study of Alzheimer's type changes. Clin Neuropathol. 17:45.Google Scholar
Hayat, M.A. 2000a. Principles and Techniques of Electron Microscopy: Biological Applications, 4th Ed. Cambridge University Press, New York and Cambridge.Google Scholar
Hayat, M A 2000b. Immunohistochemical Diagnostic Antigen Retrieval Methods. Kluwer Academic/Plenum Publishers, New York and London.Google Scholar
Holt, P.R., Moss, S.F., Kapetanakis, A.M., Petrotos, A., and Wang, S. 1997. Is Ki-67 a better proliferate marker in the colon than proliferating cell nuclear antigen? Cancer Epidemol. Biomark. Prevent. 6:131.Google Scholar
Jacobs, T.W., Prioleau, J.E., Stillman, I.E., and Schnitt, S.J. 1996. Loss of tumor marker immunostauning intensity on stored paraffin slides of breast cancer. J. Nat. Cancer Inst. 88:1054.Google ScholarPubMed
Prioleau, J.E., and Schnitt, S.J. 1995. Antigen loss in stored paraffin slides (letter). N. Enql. J. Med. 332:1521.Google Scholar
Shin, H.J.C., Kalapurakal, S.K., Lee, J,J., Ro, J.Y., Hong, W.K., and Lee, J.S. 1997. Comparison of p53 immunoreactivity in freshcut versus stored slides with and Without microwave heating. Mod. Pathol, 10:224.Google Scholar
Vis, A.N., Kranse, R., Nigg, A.L., and van der Kwast, T.H. 2000. Quantitative analysis of the decay of immunoreactivity in stored prostate needle biopsy sections. Am. J. Chin. Pathol. 113:369.Google ScholarPubMed
Wester, K., Anderson, A.C., Ranefall, P., Bengtson, E., Malmstrom, P.U., and Busch, C. 2000. Cultured human fibroblasts in agarose gel as a multi-functional control for immiinohistochernistry. Standardization of Ki-67 (MIB-1) assessment in routinely processed urinary bladder carcinoma tissue. J. Pathol. 190:503.Google Scholar