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Laser Scanning Confocal Microscopy for Materials Science

Published online by Cambridge University Press:  14 March 2018

William McMillan
William McMillan*
Affiliation:
University of Newcastle, Australia

Extract

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Confocal microscopy has gained great popularity in biology and medical research because of the ability to image three-dimensional objects at greater resolution than conventional optical microscopes. In a typical Laser Scanning Confocal Microscope (LSCM), the specimen stage is stepped up or down to collect a series of two-dimensional images (or slices) at each focal plane. Conventional light microscopes create images with a depth of field, at high power, of 2 to 3 μm. The depth of field of confocal microscopes ranges from 0.5 to 1.5 μm, which allows information to be collected from a well defined optical section rather than from most of the specimen. Therefore, due to this “thin” focal plane, out of focus light is virtually eliminated which results in an increase in contrast, clarity and detection.

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 5 , July 1998 , pp. 20 - 23
Copyright
Copyright © Microscopy Society of America 1998

References

1 Sand, D., “Scanning Force Microscopy”, Oxford University Press, 1994, p1 Google Scholar
2 Titow, W. V. & Lanham, B. J., “Reinforced Thermoplastics”, Applied Science Publishers Ltd, 1975, pp 182–185 Google Scholar
3 Whitten, A., “Abrasive Wear of Polymer Composites”, Project report, University of Newcastle, 1997, pp 6–10, p50Google Scholar
4 Friedrich, K., “Friction and Wear of Polymer Composites”, Elsevier, 1986 Google Scholar
5 Axen, N. & Hutchings, I. M., “Analysis of Abrasive Wear and Friction Behaviour of Composites”, Journal of Materials Science and Technology, Vol.12, September 1996, p757 CrossRefGoogle Scholar
6 Peng, Z., Kirk, T. B. & Xu, Z. L., “The Development of Three-Dimensional Imaging Techniques of Wear Particle Analysis”, Wear, Vol.203-204, 1997, pp418424 Google Scholar