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Increased Preservation of infrastructure With LR Gold for Immunocytochemistry

Published online by Cambridge University Press:  14 March 2018

Hiidegard H. Crowley
Hiidegard H. Crowley*
Affiliation:
University of Denver

Extract

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The acrylic embedding media such as LR White, LR Gold, and others, have long been favored over epoxide embedding media for immunocytochemistry in cases where the antigen is sparse or vulnerable to the chemical activity of epoxy monomers. Since acrylics bond through tissue and not with it as epoxides do (1), Au labeling may increase sharply when acrylics are substituted for epoxides. Unfortunately one serious disadvantage of acrylics is their reduced ability to preserve ultrastructure.

Type
Microscopy 101
Information
Microscopy Today , Volume 7 , Issue 1 , January 1999 , pp. 21
Copyright
Copyright © Microscopy Society of America 1999

References

1 Newman, G.R., and Hobot, J. A., Resin Microscopy and On-Section Immunocytochemistry, Springer Verlag, Heidelberg (1993) pp 132, 37, 36.CrossRefGoogle Scholar
2 Berryman, M.A., Rodewald, R.D., An Enhanced Method for Postembedding Immunocytochemical Staining Which Preserves Membranes.Google Scholar