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High Resolution 3-D Fluorescence Microscopy: A Comparison of Confocal Laser Scanning Microscopy and a wide-Field Deconvolution Technique

Published online by Cambridge University Press:  14 March 2018

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Advances in the biological sciences have given rise for the need to visualize microscopic structures of interest. As the requirement to see specific structure and sub-resolution details arise, investigators have turned to fluorescent probes to label and observe these details, which are often undetectable using conventional methods in light microscopy. Fluorescence microscopy offers many advantages for visualizing specific structure and sub-resolution details, which are often undetectable using transmitted light microscopy. Recent advances in fluorescent probe and microscope design, as well as imaging instrumentation designed for fluorescence applications, are now permitting life-science researchers to view details in regions of interest with increasing precision, accuracy, and resolution.

Type
Research Article
Information
Microscopy Today , Volume 5 , Issue 6 , August 1997 , pp. 10 - 13
Copyright
Copyright © Microscopy Society of America 1997

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