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DAB on Human Hepatocytes

Published online by Cambridge University Press:  14 March 2018

Charles S. Gilbert
Charles S. Gilbert*
Affiliation:
Carolinas Medical Center

Extract

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Neutrophil myeioperoxidase standard 3,3'-Diaminobenzidine Tetrahydrochioride (DAB) procedure:

When staining neutrophils, a cell suspension would be preferred, but a finely minced buffy coat can be used, if you thick section and locate the stained cells before thin sectioning. Staining should be carried out as soon as possible after fixation, The myeioperoxidase (MPO) activity falls off such that we process samples within 2-3 weeks of fixation.

Note that fixation is for 1 hour. Enzyme activity may be destroyed if tixed for longer periods.

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 2 , March 1998 , pp. 20 - 21
Copyright
Copyright © Microscopy Society of America 1998

References

References for peroxidase (DAB) staining:

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