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Confocal Imaging: In Vivo and Clinical Applications

Published online by Cambridge University Press:  14 March 2018

R.W. Beuerman ,
S.C. Kaufman  and
K.A. Palkama
R.W. Beuerman
Affiliation:
LSU Eye Center, New Orleans, LA
S.C. Kaufman
Affiliation:
LSU Eye Center, New Orleans, LA
K.A. Palkama
Affiliation:
LSU Eye Center, New Orleans, LA

Extract

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Confocal microscopy is a collection of optical techniques that are applied in a variety of hardware configurations. Design strategies for the application of these techniques have generally used laser light (Pawley, 1990). In most laboratories, basic research use has employed laser light in conjunction with a fluorescent substrate to generate an optical signal, either through direct application of a fluorescent material to cells or by the stimulation of a chromophore associated with an antibody which will identify a cellular protein under some specified experimental conditions, The use of confocal microscopy in this type of situation generally requires a standard research microscope, and the tissue may be situated on a slide or other type of container that will provide a stable, controlled environment.

Type
Research Article
Information
Microscopy Today , Volume 7 , Issue 2 , March 1999 , pp. 8 - 11
Copyright
Copyright © Microscopy Society of America 1999

References

Literature Cited

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