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Comments on “Technical Excellence in Muscle Biopsy Preparation” by Clint Lincoln (April, 1996)

Published online by Cambridge University Press:  14 March 2018

Linda R. Kitabayashi
Linda R. Kitabayashi*
Affiliation:
The Salk Institute, La Jolla, CA

Extract

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I use any type of “tin” can, of various sizes, for my isopentane container, and they are free!

Parafilm can be placed between the cork and specimen to prevent it from sticking to the cork. This is helpful when re-embedding for cross-sections. Specimens can also pinned to the cork to maintain correct lengths.

I have frozen large muscle specimens, average size about 5 cm x 2 cm x 1 cm, but they must be left in isopentane for at least one minute or even longer. If the specimen is not completely frozen, it will have “moth-eaten” fiber artifacts in the center.

After freezing large specimens, they can be put in polypropylene centrifuge tubes cooled in liquid nitrogen.

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 5 , July 1998 , pp. 14 - 15
Copyright
Copyright © Microscopy Society of America 1998