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Consumption of kiwifruit capsules increases Faecalibacterium prausnitzii abundance in functionally constipated individuals: a randomised controlled human trial

  • Paul Blatchford (a1), Halina Stoklosinski (a1), Sarah Eady (a2), Alison Wallace (a2), Christine Butts (a1), Richard Gearry (a3), Glenn Gibson (a4) and Juliet Ansell (a1)...


This study investigated the impact of ACTAZIN™ green (2400 and 600 mg) and Livaux™ (2400 mg) gold kiwifruit supplements on faecal microbial composition and metabolites in healthy and functionally constipated (FC) participants. The participants were recruited into the healthy group (n 20; one of whom did not complete the study) and the FC group (n 9), each of whom consumed all the treatments and a placebo (isomalt) for 4 weeks in a randomised cross-over design interspersed with 2-week washout periods. Modification of faecal microbiota composition and metabolism was determined by 16S rRNA gene sequencing and GC, and colonic pH was calculated using SmartPill® wireless motility capsules. A total of thirty-two taxa were measured at greater than 1 % abundance in at least one sample, ten of which differed significantly between the baseline healthy and FC groups. Specifically, Bacteroidales and Roseburia spp. were significantly more abundant (P < 0·05) in the healthy group and taxa including Ruminococcaceae, Dorea spp. and Akkermansia spp. were significantly more abundant (P < 0·05) in the FC group. In the FC group, Faecalibacterium prausnitzii abundance significantly increased (P = 0·024) from 3·4 to 7·0 % following Livaux™ supplementation, with eight of the nine participants showing a net increase. Lower proportions of F. prausnitzii are often associated with gastrointestinal disorders. The discovery that Livaux™ supplementation increased F. prausnitzii abundance offers a potential strategy for improving gut microbiota composition, as F. prausnitzii is a butyrate producer and has also been shown to exert anti-inflammatory effects in many studies.

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      Consumption of kiwifruit capsules increases Faecalibacterium prausnitzii abundance in functionally constipated individuals: a randomised controlled human trial
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (, which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corresponding author

* Corresponding author: Juliet Ansell, present address Zespri International Limited, 400 Maunganui Road, Mount Maunganui, Tauranga 3116, New Zealand, fax +64 7 572 7646, email


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1. Qin, JJ, Li, RQ, Raes, J, et al. (2010) A human gut microbial gene catalogue established by metagenomic sequencing. Nature 464, 5965.
2. Rajilic-Stojanovic, M & de Vos, WM (2014) The first 1000 cultured species of the human gastrointestinal microbiota. FEMS Microbiol Rev 38, 9961047.
3. Buffie, CG & Pamer, EG (2013) Microbiota-mediated colonization resistance against intestinal pathogens. Nat Rev Immunol 13, 790801.
4. Geurts, L, Neyrinck, AM, Delzenne, NM, et al. (2014) Gut microbiota controls adipose tissue expansion, gut barrier and glucose metabolism: novel insights into molecular targets and interventions using prebiotics. Benef Microbes 5, 317.
5. Parnell, JA & Reimer, RA (2012) Prebiotic fibres dose-dependently increase satiety hormones and alter Bacteroidetes and Firmicutes in lean and obese JCR:LA-cp rats. Br J Nutr 107, 601613.
6. Walker, AW, Ince, J, Duncan, SH, et al. (2011) Dominant and diet-responsive groups of bacteria within the human colonic microbiota. ISME J 5, 220230.
7. Wu, GD, Chen, J, Hoffmann, C, et al. (2011) Linking long-term dietary patterns with gut microbial enterotypes. Science 334, 105108.
8. Bentley-Hewitt, KL, Blatchford, PA, Parkar, SG, et al. (2012) Digested and fermented green kiwifruit increases human β-defensin 1 and 2 production in vitro . Plant Foods Hum Nutr 67, 208214.
9. Parkar, SG, Rosendale, D, Paturi, G, et al. (2012) In vitro utilization of gold and green kiwifruit oligosaccharides by human gut microbial populations. Plant Foods Hum Nutr 67, 200207.
10. Rush, EC, Patel, M, Plank, LD, et al. (2002) Kiwifruit promotes laxation in the elderly. Asia Pac J Clin Nutr 11, 164168.
11. Chan, AO, Leung, G, Tong, T, et al. (2007) Increasing dietary fiber intake in terms of kiwifruit improves constipation in Chinese patients. World J Gastroenterol 13, 47714775.
12. Hamer, HM, Jonkers, D, Venema, K, et al. (2008) Review article: the role of butyrate on colonic function. Aliment Pharmacol Ther 27, 104119.
13. Saulnier, DMA, Spinler, JK, Gibson, GR, et al. (2009) Mechanisms of probiosis and prebiosis: considerations for enhanced functional foods. Curr Opin Biotechnol 20, 135141.
14. Wong, JMW, de Souza, R, Kendall, CWC, et al. (2006) Colonic health: fermentation and short chain fatty acids. J Clin Gastroenterol 40, 235243.
15. Ansell, J, Butts, CA, Paturi, G, et al. (2015) Kiwifruit-derived supplements increase stool frequency in healthy adults: a randomized, double-blind, placebo-controlled study. Nutr Res 35, 401408.
16. Blatchford, P, Bentley-Hewitt, KL, Stoklosinski, H, et al. (2015) In vitro characterisation of the fermentation profile and prebiotic capacity of gold-fleshed kiwifruit. Benef Microbes 6, 829839.
17. Caporaso, JG, Kuczynski, J, Stombaugh, J, et al. (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7, 335336.
18. Masella, AP, Bartram, AK, Truszkowski, JM, et al. (2012) PANDAseq: paired-end assembler for illumina sequences. BMC Bioinformatics 13, 31.
19. Edgar, RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26, 24602461.
20. Caporaso, JG, Lauber, CL, Walters, WA, et al. (2011) Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proc Natl Acad Sci U S A 108, Suppl. 1, 45164522.
21. Schloss, PD, Schubert, AM, Zackular, JP, et al. (2012) Stabilization of the murine gut microbiome following weaning. Gut Microbes 3, 383393.
22. Caporaso, JG, Bittinger, K, Bushman, FD, et al. (2010) PyNAST: a flexible tool for aligning sequences to a template alignment. Bioinformatics 26, 266267.
23. DeSantis, TZ, Hugenholtz, P, Larsen, N, et al. (2006) Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl Environ Microbiol 72, 50695072.
24. Wang, Q, Garrity, GM, Tiedje, JM, et al. (2007) Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73, 52615267.
25. Richardson, AJ, Calder, AG, Stewart, CS, et al. (1989) Simultaneous determination of volatile and non-volatile acidic fermentation products of anaerobes by capillary gas-chromatography. Lett Appl Microbiol 9, 58.
26. Timm, D, Willis, H, Thomas, W, et al. (2011) The use of a wireless motility device (SmartPill®) for the measurement of gastrointestinal transit time after a dietary fibre intervention. Br J Nutr 105, 13371342.
27. Metcalf, AM, Phillips, SF, Zinsmeister, AR, et al. (1987) Simplified assessment of segmental colonic transit. Gastroenterology 92, 4047.
28. RStudio (2012) RStudio: integrated development environment for R (version 0.97.551) [computer software]. Boston, MA.
29. Benjamini, Y & Hochberg, Y (1995) Controlling the false discovery rate – a practical and powerful approach to multiple testing. J Royal Stat Soc Series B Methodol 57, 289300.
30. Rajilic-Stojanovic, M, Biagi, E, Heilig, HG, et al. (2011) Global and deep molecular analysis of microbiota signatures in fecal samples from patients with irritable bowel syndrome. Gastroenterology 141, 17921801.
31. Gilbert, JA & Alverdy, J (2016) Stool consistency as a major confounding factor affecting microbiota composition: an ignored variable? Gut 65, 12.
32. Vandeputte, D, Falony, G, Vieira-Silva, S, et al. (2016) Stool consistency is strongly associated with gut microbiota richness and composition, enterotypes and bacterial growth rates. Gut 65, 5762.
33. Lewis, SJ & Heaton, KW (1997) Increasing butyrate concentration in the distal colon by accelerating intestinal transit. Gut 41, 245251.
34. Soares, ACF, Lederman, HM, Fagundes-Neto, U, et al. (2005) Breath methane associated with slow colonic transit time in children with chronic constipation. J Clin Gastroenterol 39, 512515.
35. Taras, D, Simmering, R, Collins, MD, et al. (2002) Reclassification of Eubacterium formicigenerans Holdeman and Moore 1974 as Dorea formicigenerans gen. nov., comb. nov., and description of Dorea longicatena sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 52, 423428.
36. Kolida, S, Meyer, D & Gibson, GR (2007) A double-blind placebo-controlled study to establish the bifidogenic dose of inulin in healthy humans. Eur J Clin Nutr 61, 11891195.
37. Tuohy, KM, Kolida, S, Lustenberger, AM, et al. (2001) The prebiotic effects of biscuits containing partially hydrolysed guar gum and fructo-oligosaccharides – a human volunteer study. Br J Nutr 86, 341348.
38. Miquel, S, Martin, R, Rossi, O, et al. (2013) Faecalibacterium prausnitzii and human intestinal health. Curr Opin Microbiol 16, 255261.
39. Furet, JP, Kong, LC, Tap, J, et al. (2010) Differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers. Diabetes 59, 30493057.
40. Sokol, H, Seksik, P, Furet, JP, et al. (2009) Low counts of Faecalibacterium prausnitzii in colitis microbiota. Inflamm Bowel Dis 15, 11831189.
41. Lopez-Siles, M, Khan, TM, Duncan, SH, et al. (2012) Cultured representatives of two major phylogroups of human colonic Faecalibacterium prausnitzii can utilize pectin, uronic acids, and host-derived substrates for growth. Appl Environ Microbiol 78, 420428.
42. Carnachan, SM, Bootten, TJ, Mishra, S, et al. (2012) Effects of simulated digestion in vitro on cell wall polysaccharides from kiwifruit (Actinidia spp.). Food Chem 133, 132139.
43. Candela, M, Rampelli, S, Turroni, S, et al. (2012) Unbalance of intestinal microbiota in atopic children. BMC Microbiol 12, 95.
44. Willing, BP, Dicksved, J, Halfvarson, J, et al. (2010) A pyrosequencing study in twins shows that gastrointestinal microbial profiles vary with inflammatory bowel disease phenotypes. Gastroenterology 139, 18441854.e1.
45. Macfarlane, GT & Macfarlane, S (2011) Fermentation in the human large intestine: its physiologic consequences and the potential contribution of prebiotics. J Clin Gastroenterol 45, Suppl., S120S127.
46. Millet, S, Van Oeckel, MJ, Aluwe, M, et al. (2010) Prediction of in vivo short-chain fatty acid production in hindgut fermenting mammals: problems and pitfalls. Crit Rev Food Sci Nutr 50, 605619.
47. Walker, AW, Duncan, SH, McWilliam Leitch, EC, et al. (2005) pH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon. Appl Environ Microbiol 71, 36923700.
48. Sanchez, B, Champomier-Verges, MC, Collado Mdel, C, et al. (2007) Low-pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum . Appl Environ Microbiol 73, 64506459.


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