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Use of acetic orcein as a fast method to relax, fix, clear and stain cercariae for light microscopy

Published online by Cambridge University Press:  05 June 2009

S. C. Webb
S. C. Webb
Affiliation:
Zoology Department, University of Cape Town, Rondebosch 7700, Republic of South Africa
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Abstract

Acetic orcein stain may be dropped onto the specimen before applying a cover slip. This single reagent reveals cercarial and metacercarial structures that would otherwise be made visible only by complicated and time consuming procedures

Keywords

techniquesacetic orceinstainingcercariaeTrematoda
Type
Research Note
Information
Journal of Helminthology , Volume 65 , Issue 3 , September 1991 , pp. 234 - 238
Copyright
Copyright © Cambridge University Press 1991

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References

REFERENCES

James, B. L. (1964) The life cycle of Parvatrema homeotechnum sp. nov. (Trematoda: Digenea) and a review of the family Gymnophallidae Morozov, 1955. Parasitology, 54, 1–41.CrossRefGoogle Scholar
La Cour, L. (1941) Acetic Orcein: a new stain for chromosomes. Stain Technology, 16, 169–174.CrossRefGoogle Scholar
Stunkard, H. W. (1930) An analysis of the methods used in the study of larval trematodes. Parasitology, 22, 268–273.CrossRefGoogle Scholar
Webb, S. C. (in press). Cercaria spectorae sp. nov. (Digenea: Trematoda) found in the wedge clam Donax hanleyanus (Bivalvia) from a sandy beach on the Atlantic coast of Uruguay. Revista de Biologia del Uruguay.Google Scholar