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Intracellular proteinase of Lactococcus lactis subsp. lactis NCDO 763

Published online by Cambridge University Press:  01 June 2009

Graciela Muset ,
Véronique Monnet  and
Jean-Claude Gripon
Graciela Muset
Affiliation:
Station de Recherches Laitières, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France
Véronique Monnet
Affiliation:
Station de Recherches Laitières, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France
Jean-Claude Gripon
Affiliation:
Station de Recherches Laitières, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France
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Summary

An intracellular proteinase was purified from Lactococcus lactis subsp. lactis NCDO 763 after spheroplast formation from cell wall proteinase-deficient variants. The proteinase was active at pH 7·5 and 45 °C and affected by metalloenzyme inhibitors. Its specificity, determined on B-chain of insulin, was thermolysin-like. The B-chain of insulin was hydrolysed rapidly while hydrolysis of β-casein was slower. This enzyme has a Mr of ∽ 93000.

Type
Original articles
Information
Journal of Dairy Research , Volume 56 , Issue 5 , November 1989 , pp. 765 - 778
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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