Skip to main content Accessibility help
×
Home

Carbohydrate moieties of the interstitial and glandular tissues of the amphibian Pleurodeles waltl testis shown by lectin histochemistry

  • FRANCISCO JOSÉ SÁEZ (a1), JUAN FRANCISCO MADRID (a1), RAQUEL APARICIO (a1), FRANCISCO HERNÁNDEZ (a2) and EDURNE ALONSO (a1)...

Abstract

The amphibian testis is a useful model because of its zonal organisation in lobules, distributed along the cephalocaudal axis, each containing a unique germ cell type. Sperm empty lobules form the so-called glandular tissue at the posterior region of the gonad. Androgen production is limited to the cells of the interstitial tissue surrounding lobules with spermatozoa bundles and to the cells of the glandular tissue. In this work, we have studied the distribution of terminal carbohydrate moieties of N- and O-linked oligosaccharides in the interstitial and glandular tissue of the Pleurodeles waltl testis, by means of 14 lectins combined with chemical and enzymatic deglycosylation pretreatment. Some differences in glycan composition between the interstitial and the glandular tissue have been detected. Thus in both tissues, N-linked oligosaccharides contained mannose, Gal(β1,4)GlcNAc, and Neu5Ac(α2,3)Gal(β1,4)GlcNAc, while O-linked oligosaccharides contained Con A-positive mannose, Gal(β1,3)GalNAc, Gal(β1,4)GlcNAc, Neu5Ac(α2,3)Gal(β1,4)GlcNAc, and WGA-positive GlcNAc. Fucose was also detected in both tissues. However, GlcNAc on N-linked oligosaccharides and GalNAc and Neu5Ac(α2,6)Gal/GalNAc on both N- and O-linked oligosaccharides were found only in the interstitial tissue. As glandular tissue cells arise from the innermost cells of interstitial tissue that surround lobules, the differences in the glycan composition of interstitial and glandular tissue shown in this work may be related to the start of androgen synthesis when steroid hormone (SH)-secreting cells develop.

Copyright

Corresponding author

Correspondence to Dr Francisco José Sáez. Universidad del País Vasco, Departamento de Biología Celular y Ciencias Morfológicas, Facultad de Medicina y Odontología, B° Sarriena s/n, E-48940 Leioa (Vizcaya), Spain. Tel: +34 946 015 791; fax: +34 944 648 966; e-mail: gcpsacrf@lg.ehu.es

Keywords

Related content

Powered by UNSILO

Carbohydrate moieties of the interstitial and glandular tissues of the amphibian Pleurodeles waltl testis shown by lectin histochemistry

  • FRANCISCO JOSÉ SÁEZ (a1), JUAN FRANCISCO MADRID (a1), RAQUEL APARICIO (a1), FRANCISCO HERNÁNDEZ (a2) and EDURNE ALONSO (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.