The amphibian testis is a useful model because of its zonal organisation in lobules, distributed along the
cephalocaudal axis, each containing a unique germ cell type. Sperm empty lobules form the so-called
glandular tissue at the posterior region of the gonad. Androgen production is limited to the cells of the
interstitial tissue surrounding lobules with spermatozoa bundles and to the cells of the glandular tissue. In
this work, we have studied the distribution of terminal carbohydrate moieties of N- and O-linked
oligosaccharides in the interstitial and glandular tissue of the Pleurodeles waltl testis, by means of 14 lectins
combined with chemical and enzymatic deglycosylation pretreatment. Some differences in glycan
composition between the interstitial and the glandular tissue have been detected. Thus in both tissues,
N-linked oligosaccharides contained mannose, Gal(β1,4)GlcNAc, and Neu5Ac(α2,3)Gal(β1,4)GlcNAc, while
O-linked oligosaccharides contained Con A-positive mannose, Gal(β1,3)GalNAc, Gal(β1,4)GlcNAc,
Neu5Ac(α2,3)Gal(β1,4)GlcNAc, and WGA-positive GlcNAc. Fucose was also detected in both tissues.
However, GlcNAc on N-linked oligosaccharides and GalNAc and Neu5Ac(α2,6)Gal/GalNAc on both N-
and O-linked oligosaccharides were found only in the interstitial tissue. As glandular tissue cells arise from
the innermost cells of interstitial tissue that surround lobules, the differences in the glycan composition of
interstitial and glandular tissue shown in this work may be related to the start of androgen synthesis when
steroid hormone (SH)-secreting cells develop.