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Label-free RF biosensors for human cell dielectric spectroscopy

  • Claire Dalmay (a1), Arnaud Pothier (a1), Mathilde Cheray (a2), Fabrice Lalloue (a2), Marie-Odile Jauberteau (a2) and Pierre Blondy (a1)...


This paper presents an original biosensor chip allowing determination of intrinsic relative permittivity of biological cells at microwave frequencies. This sensor permits non-invasive cell identification and discrimination using an RF signal to probe intracellular medium of biological samples. Indeed, these sensors use an RF planar resonator that allows detection capabilities on less than 10 cells, thanks to the microscopic size of its sensitive area. Especially, measurements between 15 and 35 GHz show the ability label-free biosensors to differentiate two human cell types using their own electromagnetic characteristics. The real part of permittivity of cells changes from 20 to 48 for the nervous system cell types studied. The proposed biodetection method is detailed and we show how the accuracy and the repeatability of measurements have been improved to reach reproducible measurements.


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Corresponding author: C. Dalmay Email:


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[1]Bashir, R.: BioMEMS: state of the art in detection, opportunities and prospects. Adv. Drug Deliv. Rev., 56 (2004), 15651586.
[2]Moerner, W.E.; Orrit, M.: Illuminating single molecules in condensed matter. Science, 283 (2001), 16701676.
[3]Campbell, G.A.; Mutharasam, R.: Near real-time detection of Cryptosporidium parvum oocyst by IgM-functionalized piezoelectric-excited millimeter-sized cantilever biosensor. Biosens. Bioelectron., 23 (2007), 10391045.
[4]Kim, Y.I. et al. : Biosensors for label free detection based on RF and MEMS technology. Sens. Actuators, (2006), 592599.
[5]Kim, Y.I., Park, Y., Baik, H.K.: Development of LC resonator for label-free biomolecule detection. Sens. Actuators A: Phys., (2007).
[6]Stuchly, M.A.; Kraszewski, A.; Stuchly, S.S.; Smith, A.M.: Dielectric properties of animal tissues in vivo at radio and microwave frequencies: comparison between species. Phys. Med. Biol., 27 (1982), 927936.
[7]Blad, B.; Baldetorp, B.: Impedance spectra of tumour tissue in comparison with normal tissue; a possible clinical application for electrical impedance tomography. Physiol. Meas., 17 (1996), A 105A 115.
[8]Vander Vorst, A.; Rosen, A.; Kotsuka, Y.: RF/Microwave Interaction with Biological Tissues, IEEE Press, ISBN-10-13, 2006.
[9]Gabriel, S.; Lau, S.W.; Gabriel, C.: The dielectric properties of biological tissues : II. Measurements in the frequency range 10 Hz to 20 GHz. Phys. Med. Biol., 41 (1996), 22512269.
[10]Burdette, E.C.; Cain, F.L.; Seals, J.: In vivo probe measurement technique for determining dielectric properties at VHF through microwave frequencies. IEEE Trans. Microwave Theory Tech., MTr-28 (4) (1980).
[11]Tofighi, M.R.; Daryoush, A.S.: Study of the activity of neurological cell solutions using complex permittivity measurement, In IEEE MTT-S Int. Microwave Symp. Digest, 2002.
[12]Song, C.; Wang, P.: A radio frequency device for measurement of minute dielectric property changes in microfluidic channels. Appl. Phys. Lett., 94 (2009).
[13]Richards Grayson, A.C. et al. : A BioMEMS review: MEMS technology for physiologically integrated devices. Proc. IEEE, 92 (1) (2004), 621.
[14]Cheung, K.C.; Renaud, P.: BioMEMS for medicine: on-chip cell characterization and implantable microelectrodes. Solid-State Electron., 50 (2006), 551557.
[15]Treizebré, A.; Akalin, T.; Bocquet, B.: Planar excitation of Goubau transmission line for THz BioMEMS. IEEE Microwave Wirel. Compon.. Lett., 15 (12) (2005), 886888.
[16]Denef, N. et al. : RF detection of DNA based on CMOS inductive and capacitive sensors, in EUMW Conf. Digest, September 2004, 669672.
[17]Dalmay, C.; Pothier, A.; Blondy, P.; Lalloue, F.; Jauberteau, M-O.: Label free biosensor for human cell characterization using radio and microwave frequencies, in IEEE MTT-S Int. Microwave Symp. Digest, 2008.
[18]Dalmay, C.; Pothier, A.; Blondy, P.; Cheray, M.; Lalloué, F.; Jauberteau, M-O.: RF Biosensor based on microwave filter for biological cell characterisation, in 39th European Microwave Conf., EuMC03-1, Roma, Italy, October 2009.


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Label-free RF biosensors for human cell dielectric spectroscopy

  • Claire Dalmay (a1), Arnaud Pothier (a1), Mathilde Cheray (a2), Fabrice Lalloue (a2), Marie-Odile Jauberteau (a2) and Pierre Blondy (a1)...


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