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1831 – Dnase And Protease Activity Of Immunoglobulins g Of Patients With Schizophrenia

Published online by Cambridge University Press:  15 April 2020

L. Smirnova ,
N. Fattakhov ,
D. Parshukova ,
V. Buneva ,
E. Kornetova ,
A. Semke  and
S. Ivanova
L. Smirnova
Affiliation:
Laboratory of Cellular and Molecular-Biological Investigations, Mental Health Research Institute, Tomsk, Russia
N. Fattakhov
Affiliation:
Medico-Biological Faculty, Siberian State Medical University, Tomsk, Russia
D. Parshukova
Affiliation:
Medico-Biological Faculty, Siberian State Medical University, Tomsk, Russia
V. Buneva
Affiliation:
Laboratory of Repair Enzymes, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia
E. Kornetova
Affiliation:
Mental Health Research Institute, Tomsk, Russia
A. Semke
Affiliation:
Mental Health Research Institute, Tomsk, Russia
S. Ivanova
Affiliation:
Laboratory of Cellular and Molecular-Biological Investigations, Mental Health Research Institute, Tomsk, Russia

Abstract

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Introduction

Phenomenon of presence of catalytic properties in immunoglobulins has been actively investigated. Research of catalytic properties of antibodies in schizophrenic patients with various clinical manifestations of the illness has not been carried out previously.

Objectives

To study DNase and proteolytic activity of immunoglobulins G isolated from serum of blood of patients with schizophrenia.

Methods

IgG have been isolated from serum of blood of 34 persons with affine chromatography. DNase activity of antibodies was identified according to degree of hydrolysis of supercoiled form of DNA of plasmid pBluescript per unit of time. Proteolytic activity was assessed according to degree of hydrolysis of basic protein myelin and its peptides. IgG were electrophoretic homogeneity.

Results

We have identified that IgG of schizophrenic patients possess a higher (0,392 ± 0,19 nM DNA /mgAB/h), than in healthy persons (0,029 ± 0,06 nM DNA /mgAB/h) specific DNAse activity. It has been shown that DNase activity is an own property of antibodies. It has been found that proteolytic hydrolysis of serum of blood of schizophrenic patients is 5 times as high as indices of hydrolysis in healthy persons. It has been revealed that patients with predominant negative symptoms show maximum high percent of protease hydrolysis 66,9%, different from patients with leading positive symptoms (13,9%).

Conclusions

Increase of DNA-hydrolyzing properties of Ig G is likely to be associated with great number of extracellular DNA. Detection of high values of proteolytic activity may be associated with occurring in serum of blood of patients of large number of destructed or damaged proteins.

Type
Abstract
Information
European Psychiatry , Volume 28 , Issue S1: Abstracts of the 21th European Congress of Psychiatry , 2013 , 28-E1083
Copyright
Copyright © European Psychiatric Association 2013
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