In the fourth and early fifth centuries Christians laid claim to the land of Palestine. The purpose of this paper is to analyze the investment of the land of Palestine and its places with Christian historical and cultural meanings, and to trace its remapping as the “holy land.” This map was not a figurative representation of geographical and cultural features; as with all maps, it was an idea. The Christian “holy land” is also an idea, one which did not exist at the beginning of the fourth century, but which, by the mid-fifth century, was a place constructed of a rich texture of places, beliefs, actions, and texts, based in the notion that the landscape provided evidence of biblical truths. When Constantine became a Christian, there was no “holy land”; however, over the succeeding one hundred and thirty years Christians marked and identified many of their holy places in Palestine. The map-makers in this transformation were emperors, bishops, monastics, holy women, and pilgrims who claimed the holy places for Christianity, constructing the land as topographically Christian and mediating this view of their world through their pilgrim paths, buildings, liturgies, and texts. The idea of mapping is used here as an aid to understanding the formation of cultural viewpoints and the validation of ideas and actions that informed the construction of the “holy land.”