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Magnetic Resonance Imaging and 31P Magnetic Resonance Spectroscopy Study of the Effect of Temperature on Ischemic Brain Injury

  • Garnette R. Sutherland (a1), Howard Lesiuk (a1), Paul Hazendonk (a1), James Peeling (a1), Richard Buist (a1), Piotr Kozlowski (a1), Andrzej Jazinski (a1) and John K. Saunders (a1)...

Abstract:

Transient forebrain ischemia was induced in rats whose brain temperature was 31, 33, 35, 38, or 40°C. The development of regional injury was followed using magnetic resonance (MR) imaging, with the ultimate extent of neuronal injury quantified histopathologically. Animals in the hypothermic groups showed minimal changes in MR images over 4 days; normothermic animals snowed intensity enhancement attributed to progressive edema developing in the striatum and, later, in the hippocampus. Ischemia at 40°C resulted in widespread edema formation by I day post-ischemia; animals in this group did not survive beyond 30 hours. Histopathological analysis at 4 days (1 day for the hyperthermic group) post-ischemia showed that neuronal damage in the normothermic group was confined to the hippocampus and striatum. Minimal damage was found in the hypothermic groups; damage in the hyperthermic group was severe throughout the forebrain. There were no differences in the pre-ischemia 31P MR spectra for the different groups. During ischemia, the increase in intensity of the Pi peak and the fall in tissue pH increased with temperature in the order hypothermic < normothermic < hyperthermic group of animals. Post-ischemia energy recovery was similar in all groups, while pH recovered more rapidly in hypothermic animals.

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Copyright

Corresponding author

Department of Pharmacology, The University of Manitoba, 770 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0W3

References

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1.Botterell, EH, Lougheed, WM, Scott, JW, et al. Hypothermia and interruption of carotid or carotid and vertebral circulation in the surgical management of intracranial aneurysms. J Neurosurg 1956; 13: 142.
2.Botterell, EH, Lougheed, WM, Morley, TP, et al. Hypothermia in the surgical treatment of ruptured intracranial aneurysms. J Neurosurg 1958; 15: 418.
3.Adams, JE.Value of hypothermia and arterial occlusion in the treatment of intracranial aneurysms. Surg Gynec and Obst 1959; 108: 631635.
4.Connolly, JE, Boyd, RJ, Calvin, JW.The protective effect of hypothermia in cerebral ischemia: experimental and clinical application by selective brain cooling in the human. Surgery 1962; 52: 1524.
5.Busto, R, Dietrich, WD, Globus, MY, et al. The importance of brain temperature in cerebral ischemic injury. Stroke 1989; 20: 11131114.
6.Busto, R, Globus, MY, Dietrich, WD, et al. Effect of mild hypothermia on ischemia-induced release of neurotransmitters and free fatty acids in rat brain. Stroke 1989; 20: 904910.
7.Clifton, GL, Jiang, JY, Lyeth, BG, et al. Marked protection by moderate hypothermia after experimental traumatic brain injury. J Cereb Blood Flow Metab 1991; 11: 114121.
8.Dietrich, WD, Busto, R, Valdês, I, et al. Effects of normothermic versus mild hyperthermic forebrain ischemia in rats. Stroke 1990; 21: 13181325.
9.Kuroiwa, T, Bonnekoh, P, Hossmann, K-A.Prevention of post ischemia hyperthermia prevents ischemic injury of CAI neurons in gerbils. J Cereb Blood Flow Metab 1990; 10: 550556.
10.Minamisawa, H, Smith, ML, Siesjo, BK.The effect of mild hyperthermia and hypothermia on brain damage following 5, 10, and 15 minutes of forebrain ischemia. Ann Neurol 1990; 28: 2633.
11.Welsh, FA, Sims, RE, Harris, VA.Mild hypothermia prevents ischemic injury in gerbil hippocampus. J Cereb Blood Flow Metab 1990; 10: 557563.
12.Cornish-Bowden, A.Fundamentals of enzyme kinetics. Toronto, Butterworths and Co (Canada) Ltd 1979; 130145.
13.Rosomoff, HL, Holaday, DA.Cerebral blood flow and cerebral oxygen consumption during hypothermia. Am J Physiol 1954; 179: 8588.
14.Lougheed, WM, Khan, DS.Circumvention of anoxia during arrest of cerebral circulation for intracranial surgery. J Neurosurg 1955; 12: 226239.
15.Siesjo, BK.Cerebral circulation and metabolism. J Neurosurg 1984; 60: 883908.
16.Bryan, RN, Willcott, MR, Schneiders, NJ, et al. NMR evaluation of stroke in the rat. Am J Neuroradiol 1983; 4: 242.
17.Dempsey, RJ, Combs, DJ, Maley, ME, et al. Moderate hypothermia reduces post ischemic edema development and leukotriene production. Neurosurgery 1987; 21: 177181.
18.Sutherland, GR, Lesiuk, H, Peeling, J, et al. Experimental cerebral ischemia studied using nuclear magnetic resonance imaging and spectroscopy. J Can Assoc Radiol 1990; 41: 2431.
19.Sutherland, GR, Peeling, J, Lesiuk, H, et al. The effects of caffeine on ischemic neuronal injury as determined by magnetic resonance imaging and histopathology. Neuroscience 1991; 42: 171182.
20.Inoue, Y, Takemoto, K, Miyamoto, T, et al. Sequential computed tomography scans in acute cerebral infarction. Radiology 1980; 135: 655.
21.Levy, RM, Mano, I, Brito, A, et al. NMR imaging of acute experimental cerebral ischemia: time course and pharmacologic manipulations. Am J Neuroradiol 1983; 4: 238.
22.Peeling, J, Wong, D, Sutherland, GR.Nuclear magnetic resonance study of regional metabolism after forebrain ischemia in rats. Stroke 1989; 20: 633640.
23.Sutherland, GR, Lesiuk, H, Bose, R, et al. The effect of mannitol nimodipine and indomethacin singly or in combination on cerebral ischemia in rats. Stroke 1988 19: 571578.
24.Petroff, OAC, Prichard, JW, Behar, KL, et al. Cerebral intracellular pH by 31P nuclear magnetic resonance spectroscopy. Neurology 1985; 35: 781788.
25.Smith, ML, Auer, RN, and Seisjo BK. The density and distribution of ischemic brain injury in the rat following 2-10 minutes of forebrain ischemia. Acta Neuropathol (Beri.) 1984; 64: 319332.
26.Siesjo, BK.Cell damage in the brain: a speculative synthesis. J Cereb Blood Flow Metab 1981; 1: 155185.
27.Harris, RJ, Symon, L.Extracellular, pH, potassium and calcium activities in progressive ischemia of rat cortex. J Cereb Blood Flow Metab 1981; 1: 203209.
28.Yanagihara, R, McCall, JT.Ionic shift in cerebral ischemia. Life Sci 1982; 30: 19211925.
29.Cheung, JY, Bonventre, JV, Malis, CD, et al. Calcium and ischemic injury. N Engl J Med 1986; 314: 16701676.
30.de-Boer, J, Klein, HC, Postema, F, et al. Rat striatal cation shifts reflecting hypoxic-ischemic damage can be predicted by on-line impedance measurements. Stroke 1989; 20: 13771382.
31.Buchan, A, Pulsinelli, WA.Hypothermia but not the N-methyl-Daspartate antagonist MK-801, attenuates neuronal damage in gerbils subjected to transient global ischemia. J Neurosci 1990; 10: 311316.
32.Corbett, D, Evans, S, Thomas, C, et al. MK-801 reduces cerebral ischemic injury by inducing hypothermia. Brain Res 1990; 514: 300304.
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Canadian Journal of Neurological Sciences
  • ISSN: 0317-1671
  • EISSN: 2057-0155
  • URL: /core/journals/canadian-journal-of-neurological-sciences
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