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In Vitro31P NMR Spectroscopy Detects Altered Phospholipid Metabolism in Alzheimer's Disease

Published online by Cambridge University Press:  18 September 2015

Orietta Miatto ,
R. Gilberto Gonzalez ,
Ferdinando Buonanno  and
John H. Growdon
Orietta Miatto*
Affiliation:
Massachusetts General Hospital, Boston, MA
R. Gilberto Gonzalez
Affiliation:
Massachusetts Institute of Technology, Cambridge, MA
Ferdinando Buonanno
Affiliation:
Massachusetts General Hospital, Boston, MA
John H. Growdon
Affiliation:
Massachusetts General Hospital, Boston, MA
*
Massachusetts General Hospital, Boston, MA 02114, U.S.A.
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Abstract:

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In order to study possible metabolic derangements in Alzheimer's disease (AD), we performed phosphorus 31 nuclear magnetic resonance (31P NMR) spectroscopy on brain samples obtained at autopsy from 7 patients with AD and 9 control subjects. Aqueous solutions of brain tissue contained well-defined peaks of intermediate compounds in phospholipid metabolism, including the phosphomonoesters phosphocholine and phosphoethanolamine, and the phosphodiesters glycerophosphorylcholine and glycerophosphorylethanolamine. 3IP NMR spectra also displayed the inorganic phosphorus signal, which provides an index to the in vivo concentration of high-energy compounds.

We found evidence for altered phospholipid metabolism in that relative levels of phosphomonoesters were decreased, and phosphodiesters increased, in frontal and parietal regions of patients with AD compared to control subjects. The inorganic phosphorus resonance peaks were similar in AD and control subjects, suggesting that energy stores are not diminished in AD. These preliminary data are consistent with the hypothesis that abnormalities in phospholipid metabolism contribute to possible neuronal membrane dysfunction and impaired cholinergic neurotransmission in AD.

Type
Imaging of Demented Subjects
Information
Canadian Journal of Neurological Sciences , Volume 13 , Issue S4 , November 1986 , pp. 535 - 539
Copyright
Copyright © Canadian Neurological Sciences Federation 1986

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