Skip to main content Accessibility help
×
Home

Clinical, neuropathological and molecular features of fatal human pegivirus-associated encephalitis

Published online by Cambridge University Press:  05 September 2019

LM Schmitt
Affiliation:
Departments of Laboratory Medicine & Pathology Neuroscience & Mental Health Institute, University of Alberta, Edmonton, Alberta, Canada
E Balcom
Affiliation:
Medicine
M Doan
Affiliation:
Medicine
WG Branton
Affiliation:
Medicine
J Jovel
Affiliation:
Cell Biology
G Blevins
Affiliation:
Medicine
B Edguer
Affiliation:
Medicine
TC Hobman
Affiliation:
Cell Biology
E Yacyshyn
Affiliation:
Medicine
D Emery
Affiliation:
Radiology & Diagnostic Imaging
A Box
Affiliation:
Departments of Laboratory Medicine & Pathology
C Power
Affiliation:
Medicine Radiology & Diagnostic Imaging
FKH van Landeghem
Affiliation:
Departments of Laboratory Medicine & Pathology Radiology & Diagnostic Imaging
Rights & Permissions[Opens in a new window]

Abstract

Flaviviruses include many viruses causing encephalitis, including West Nile encephalitis, St. Louis encephalitis, tick-borne encephalitis and Japanese encephalitis. Human pegivirus genotype-1 (HPgV-1) is a lesser known member of the Flaviviridae family and has been identified in human serum, cerebrospinal fluid and brain tissue. Here, we describe two adult patients with fatal HPgV-1-associated encephalitis. Neuroimaging revealed multifocal lesions, initially present in the periventricular and brain stem white matter, then one year later throughout the corona radiata bilaterally with marked involvement of the brainstem and cervical spinal cord. Phylogenetic analyses of HPgV-1 showed clustering of brain-derived sequences from both patients with other human pegiviruses. In both patients, a novel 87-nucleotide deletion in the viral NS2 gene was detected. The presence of positive and negative strand HPgV-1 RNA and viral antigens in both patients indicated viral persistence and replication in the CNS. Autopsy showed lymphocyte infiltration and gliosis predominantly in white matter of the brain and brain stem but, to a lesser extent, also in grey matter. Immunofluorescence revealed HPgV-1 NS5A antigen in lymphocytes as well as in astrocytes and oligodendrocytes. Thus, we hypothesize that the novel deletion in the NS2 coding region may have caused HPgV-1 neuroadaptation or might represent a yet unrecognized genotype of human pegivirus.

LEARNING OBJECTIVES

This presentation will enable the learner to:

  1. 1.Describe the clinical and neuropathological features of fatal human pegivirus-associated encephalitis
  2. 2.Recognize the importance of molecular analysis in encephalitis cases with unknown etiology

Type
Abstracts
Copyright
© The Canadian Journal of Neurological Sciences Inc. 2019 

Full text views

Full text views reflects PDF downloads, PDFs sent to Google Drive, Dropbox and Kindle and HTML full text views.

Total number of HTML views: 0
Total number of PDF views: 68 *
View data table for this chart

* Views captured on Cambridge Core between 05th September 2019 - 25th January 2021. This data will be updated every 24 hours.

Access
Hostname: page-component-898fc554b-z76t5 Total loading time: 0.349 Render date: 2021-01-25T19:57:57.848Z Query parameters: { "hasAccess": "1", "openAccess": "0", "isLogged": "0", "lang": "en" } Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false }

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Clinical, neuropathological and molecular features of fatal human pegivirus-associated encephalitis
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Clinical, neuropathological and molecular features of fatal human pegivirus-associated encephalitis
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Clinical, neuropathological and molecular features of fatal human pegivirus-associated encephalitis
Available formats
×
×

Reply to: Submit a response


Your details


Conflicting interests

Do you have any conflicting interests? *