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Assessment of Genetic Polymorphisms in DNA from Formalin Fixed Neurological Tissues

Published online by Cambridge University Press:  18 September 2015

M. Mordila ,
G. Vaula  and
P.H. St George-Hyslop
M. Mordila*
Affiliation:
Division of Neurology, Department of Medicine, University of Toronto, and the Center for Research in Neurodegenerative Disease. University of Toronto, Toronto Department of Neurological and Psychiatric Sciences, University of Florence, Firenze, Italy
G. Vaula
Affiliation:
Division of Neurology, Department of Medicine, University of Toronto, and the Center for Research in Neurodegenerative Disease. University of Toronto, Toronto Department of Neurology, University of Turin, Torino, Italy
P.H. St George-Hyslop
Affiliation:
Division of Neurology, Department of Medicine, University of Toronto, and the Center for Research in Neurodegenerative Disease. University of Toronto, Toronto
*
Center for Research in Neurogenerative Diseases, Tanz Neuroscience Building, University of Toronto. 6 Queen’s Park Crescent, Toronto, Ontario, Canada M5S 1A8
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Abstract:

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The ability to analyze the genotype of deceased affected members of pedigrees segregating inherited neurological diseases considerably augments the informativeness of such pedigrees. This information has direct application in attempts to isolate disease genes by positional cloning strategies, and for genetic counselling. We show that the genotype at polymorphic simple sequence repeat loci can be determined from genomic DNA isolated from 10 micron thick paraffin embedded, formalin fixed neurological tissues. The critical constraint on this method is the size of the template target bearing the simple sequence repeat, which should ideally be less than 165 base pairs.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 21 , Issue 3 , August 1994 , pp. 248 - 251
Copyright
Copyright © Canadian Neurological Sciences Federation 1994

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