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Weaning affects lipoprotein lipase activity and gene expression in adipose tissues and in masseter but not in other muscles of the calf

Published online by Cambridge University Press:  09 March 2007

Jean-François Hocquette ,
Benoît Graulet ,
Michel Vermorel  and
Dominique Bauchart
Jean-François Hocquette*
Affiliation:
Unité de Recherches sur les Herbivores, Institut National de la Recherche Agronomique, Centre de Recherches de Clermont Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
Benoît Graulet
Affiliation:
Unité de Recherches sur les Herbivores, Institut National de la Recherche Agronomique, Centre de Recherches de Clermont Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
Michel Vermorel
Affiliation:
Unité de Recherches sur les Herbivores, Institut National de la Recherche Agronomique, Centre de Recherches de Clermont Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
Dominique Bauchart
Affiliation:
Unité de Recherches sur les Herbivores, Institut National de la Recherche Agronomique, Centre de Recherches de Clermont Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
*
*Corresponding author: Dr Jean-François Hocquette, fax +33 4 73 62 46 39, email hocquet@clermont.inra.fr
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Abstract

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The nutritional and physiological modifications that occur during the weaning period induce adaptations of tissue metabolism in all mammal species. Among the adaptations due to weaning in ruminants, the regulation of lipoprotein lipase (LPL) activity, one of the rate-limiting steps of fatty acid utilization by tissues, was still unknown. The present study aimed at comparing LPL activity and gene expression in the heart, seven skeletal muscles and three adipose tissue sites between two groups of seven preruminant (PR) or ruminant (R) calves having a similar age (170 d), similar empty body weight (194 kg) at slaughter, and similar net energy intake from birth onwards. Triacylglycerol content of adipose tissues was 16 % lower in R than in PR calves, (P<0·01). This could be partly the result from a lower LPL activity (-57 %, P<0·01). LPL mRNA levels were also lower in R calves (-48 % to -68 %, P<0·01) suggesting a pretranslational regulation of LPL activity. Activity and mRNA levels of LPL did not differ significantly in the heart and skeletal muscles except in the masseter in which LPL activity and mRNA levels were higher (+50 % and +120 % respectively, P<0·01) in the R calves. Regulation of LPL in masseter could be explained by the high contractile activity of this muscle after weaning due to solid food chewing. In conclusion, weaning in the calf affects LPL activity and expression in adipose tissues, but not in skeletal muscles except the masseter.

Keywords

Lipoprotein lipaseMuscleAdipose tissueWeaningBovine
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 4 , October 2001 , pp. 433 - 441
Copyright
Copyright © The Nutrition Society 2001

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