Skip to main content Accessibility help
×
Home

Virgin olive oil administration improves the effect of aspirin on retinal vascular pattern in experimental diabetes mellitus

  • Jose Pedro De La Cruz (a1), Sergio Del Río (a2), Juan Antonio López-Villodres (a1), Maria Auxiliadora Villalobos (a3), Nuria Jebrouni (a1) and Jose Antonio González-Correa (a1)...

Abstract

The aim of the present study is to evaluate the possible influence of virgin olive oil (VOO) on the effect of acetylsalicylic acid (ASA) in platelet aggregation, prostanoid and NO production and retinal vascular pattern in rats with experimental type 1-like diabetes. We used 100 male Wistar rats that were distributed into five groups: (1) non-diabetic rats (NDR); (2) untreated diabetic rats (DR); (3) DR treated with ASA (2 mg/kg per d per os (p.o.)); (4) DR treated with VOO (0·5 ml/kg per d p.o.); (5) DR treated with ASA plus VOO. The duration of diabetes was 3 months, and each treatment was administered from the first day of diabetes. Variables that were quantified were platelet aggregation (Imax), thromboxane B2 (TxB2), aortic prostacyclin (6-keto-PGF) and NO, and the percentage of retina with horseradish peroxidase-permeable vessels (HRP-PV). Diabetic rats showed a higher Imax (35 %) and TxB2 (63 %) than NDR, and a lower 6-keto-PGF, NO and HRP-PV than NDR ( − 74·6 %). ASA and VOO administration reduced these differences and prevented the percentage of HRP-PV ( − 59·7 % with ASA and − 46·7 % with VOO). The administration of ASA plus VOO showed a strong platelet inhibition (80·2 v. 23·4 % for VOO and 50·6 % for ASA+VOO, P < 0·0001), and reduced HRP-PV differences to − 31·6 % (P < 0·001 with respect to DR and P < 0·0001 with respect to DR treated with ASA). In conclusion, the administration of VOO to rats with type 1-like diabetes mellitus improves the pharmacodynamic profile of ASA, and increases its retinal anti-ischaemic effect.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Virgin olive oil administration improves the effect of aspirin on retinal vascular pattern in experimental diabetes mellitus
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Virgin olive oil administration improves the effect of aspirin on retinal vascular pattern in experimental diabetes mellitus
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Virgin olive oil administration improves the effect of aspirin on retinal vascular pattern in experimental diabetes mellitus
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Jose Antonio González-Correa, fax +34 952 131568, email jpcruzcortes@yahoo.es

References

Hide All
1Carr, ME (2001) Diabetes mellitus: a hypercoagulable state. J Diabetes Complications 15, 4454.
2Colwell, JA & Nesto, RW (2003) The platelet in diabetes: focus on prevention of ischemic events. Diabetes Care 26, 21812188.
3De la Cruz, JP, Moreno, A, Muñoz, M, et al. (1997) Effect of aspirin plus dipyridamole on the retinal vascular pattern in experimental diabetes mellitus. J Pharmacol Exp Ther 280, 454459.
4Reiss, AB & Edelman, SD (2006) Recent insights into the role of prostanoids in atherosclerotic vascular disease. Curr Vasc Pharmacol 4, 395408.
5De La Cruz, JP, Moreno, A, Guerrero, A, et al. (2002) Nitric oxide–cGMP and prostacyclin–cAMP pathways in patients with type II diabetes and different types of retinopathy. Pathophysiol Haemost Thromb 32, 2532.
6De La Cruz, JP, Moreno, A, Guerrero, A, et al. (2001) Antiplatelet effects of prostacyclin and nitric oxide in patients with type I diabetes and ischemic or edematous retinopathy. Platelets 12, 210217.
7Schalkwijk, CG & Stehouwer, CD (2005) Vascular complications in diabetes mellitus: the role of endothelial dysfunction. Clin Sci (Lond) 109, 143159.
8De La Cruz, JP, Moreno, A, Sanchez de la Cuesta, F, et al. (1990) Effect of antiplatelet drug therapy on the retinal vascular pattern in experimental diabetes mellitus. Can J Ophthalmol 25, 329332.
9De La Cruz, JP, Guerrero, A, Paniego, MJ, et al. (2002) Effect of aspirin on prostanoids and nitric oxide production in streptozotocin-diabetic rats with ischemic retinopathy. Naunyn Schmiedebergs Arch Pharmacol 365, 96101.
10De La Cruz, JP, Moreno, A, Mérida, F, et al. (1996) The pyrimido-pyrimidine derivatives, dipyridamole, mopidamol and RA-642, prevent from retinal vascular defects in experimental diabetes mellitus. Thromb Res 81, 327337.
11Moreno, A, De La Cruz, JP, Mérida, F, et al. (1995) Effects of ditazol on the vascular retinal pattern in experimental diabetes in rats. Haemostasis 25, 166171.
12De La Cruz, JP, Moreno, A, Ruiz-Ruiz, MI, et al. (1998) Effect of camonagrel, a selective thromboxane synthase inhibitor, on retinal vascularization in experimental diabetes. Eur J Pharmacol 350, 8185.
13De La Cruz, JP, Moreno, A, Ruiz-Ruiz, MI, et al. (2000) Effect of DT-TX 30, a combined thromboxane synthase inhibitor and thromboxane receptor antagonist, on retinal vascularity in experimental diabetes mellitus. Thromb Res 97, 125131.
14De La Cruz, JP, Arrebola, M, González-Correa, JA, et al. (2003) Effects of clopidogrel and ticlopidine on experimental diabetic ischemic retinopathy in rats. Naunyn Schmiedebergs Arch Pharmacol 367, 204210.
15Pérez-Jiménez, F, Ruano, J, Pérez-Martínez, P, et al. (2007) The influence of olive oil on human health: not a question of fat alone. Mol Nutr Food Res 51, 11991208.
16Covas, MI (2007) Olive oil and the cardiovascular system. Pharmacol Res 55, 175186.
17González-Correa, JA, Muñoz-Marín, J, Arrebola, MM, et al. (2007) Dietary virgin olive oil reduces oxidative stress and cellular damage in rat brain slices subjected to hypoxia–reoxygenation. Lipids 42, 921929.
18Glassman, AB (1993) Platelet abnormalities in diabetes mellitus. Ann Clin Lab Sci 23, 4750.
19Schror, K (1997) Blood vessel wall interactions in diabetes. Diabetes 46, Suppl. 2, 115118.
20Giugliano, D, Ceriello, A & Paolisso, G (1996) Oxidative stress and diabetic vascular complications. Diabetes Care 19, 257267.
21Tesfamariam, B & Cohen, RA (1992) Role of superoxide anion and endothelium in vasoconstrictor action of prostaglandin endoperoxide. Am J Physiol 262, H1915H1919.
22Chakrabarti, S, Cukiernik, M, Hileeto, D, et al. (2000) Role of vasoactive factors in the pathogenesis of early changes in diabetic retinopathy. Diabetes Metab Res Rev 16, 393407.
23De Vriese, AS, Verbeuren, TJ, Van de Voorde, J, et al. (2000) Endothelial dysfunction in diabetes. Br J Pharmacol 130, 963974.
24De la Cruz, JP, Moreno, A, Muñoz, M, et al. (1997) Effect of aspirin plus dipyridamole on the retinal vascular pattern in experimental diabetes mellitus. J Pharmacol Exp Ther 280, 454459.
25González-Correa, JA, Arrebola, MM, Guerrero, A, et al. (2006) Antioxidant and antiplatelet effects of the alpha-tocopherol-aspirin combination in type 1-like diabetic rats. Life Sci 79, 14051412.
26Vane, JR (1971) Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol 231, 232235.
27López-Farré, A, Caramelo, C, Esteban, A, et al. (1995) Effect of aspirin on platelet–neutrophil interaction. Role of nitric oxide and endothelin-1. Circulation 91, 20802088.
28De La Cruz, JP, Blanco, E & Sánchez de la Cuesta, F (2000) Effect of dipyridamole and aspirin on the platelet–neutrophil interaction via the nitric oxide pathway. Eur J Pharmacol 397, 3541.
29Madajka, M, Korda, M, White, J, et al. (2003) Effect of aspirin on constitutive nitric oxide synthase and the bioavailability of NO. Thromb Res 110, 317321.
30Visioli, F, Caruso, D, Grande, S, et al. (2005) Virgin Olive Oil Study (VOLOS): vasoprotective potential of extra virgin olive oil in mildly dyslipidemic patients. Eur J Nutr 44, 121127.
31González-Correa, JA, Arrebola, MM, Guerrero, A, et al. (2006) Antioxidant and antiplatelet effects of the alpha-tocopherol–aspirin combination in type 1-like diabetic rats. Life Sci 79, 14051412.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed