Hostname: page-component-848d4c4894-pjpqr Total loading time: 0 Render date: 2024-07-01T02:54:29.740Z Has data issue: false hasContentIssue false
  • English
  • Français

The use of deuterium oxide space to determine the amount of body fat in pregnant Blackface ewes

Published online by Cambridge University Press:  09 March 2007

Janet Z. Foot  and
J. F. D. Greenhalgh
Janet Z. Foot
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
J. F. D. Greenhalgh
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The use of deuterium oxide dilution to obtain an indirect estimate of body water in pregnant ewes was examined and the value of such an estimate in predicting body fat was investigated.

2. Deuterium oxide was infused intravenously into fourteen twin-bearing Blackface ewes and blood samples were taken 6–7 h later. The animals were subsequently slaughtered.

3. Deuterium oxide concentration was estimated by infrared spectroscopy and the deuterium oxide space was calculated. Studies on dose rates and equilibration times were made with the Blackface ewes and some non-pregnant Dorset ewes.

4. The amount of body fat in each ewe was estimated from the deuterium oxide space using a prediction equation relating body fat to body water in twenty-five pregnant and non-pregnant ewes which had been slaughtered.

5. Once satisfactory measurement of deuterium oxide concentration had been achieved, body fat predicted from deuterium oxide space differed from values obtained by analysis of the slaughtered animals by -0.8 to +1.7 kg in seven ewes containing 5.2–21.4 kg fat.

6. The standard deviation from regression of percentage of body fat estimated from deuterium oxide space on percentage of body fat measured after slaughter was ±1.2 percentage units. Thus deuterium oxide space was used to provide a useful measure of body fat in pregnant ewes.

Type
Research Article
Information
British Journal of Nutrition , Volume 24 , Issue 3 , September 1970 , pp. 815 - 825
Copyright
Copyright © The Nutrition Society 1970

References

REFERENCES

Anbar, M. & Lewitus, Z. (1958). Nature, Lond. 181, 344.CrossRefGoogle Scholar
Carnegie, A. B. & Tulloh, N. M. (1968). Proc. Aust. Soc. Anim. Prod. 7, 308.Google Scholar
Flanagan, B. (1964). Clin. Sci. 27, 335.Google Scholar
Foot, J. Z. (1969). J. Reprod. Fert. Suppl. 9, p. 9.Google Scholar
Hytten, F. E., Taggart, N., Billewicz, W. Z. & Jason, A. C. (1962). Physics Med. Biol. 6, 415.CrossRefGoogle Scholar
Panaretto, B. A. (1963). Aust. J. agric. Res. 14, 944.CrossRefGoogle Scholar
Panaretto, B. A. (1964). Aust. J. agric. Res. 15, 771.CrossRefGoogle Scholar
Panaretto, B. A. & Till, A. R. (1963). Aust. J. agric. Res. 14, 926.CrossRefGoogle Scholar
Reid, J. T., Balch, C. C. & Glascock, R. F. (1958). Br. J. Nutr. 12, 43.CrossRefGoogle Scholar
Reid, J. T., Bensadoun, A., Bull, L. S., Burton, J. H., Gleeson, P. A., Han, I. K., Joo, Y. D., Johnson, D. E., McManus, W. R., Paladines, O. L., Stroud, J. W., Tyrrell, H. F., VanNiekerk, B. D. H., Wellington, G. H. & Wood, J. D. (1968). Proc. Cornell Nutr. Conf. Feed Mfrs p. 18.Google Scholar
Schloerb, P. R., Friis-Hansen, B. J., Edelman, I. S., Sheldon, D. B. & Moore, F. D. (1951). J. Lab. clin. Med. 37, 653.Google Scholar
Schloerb, P. R., Friis-Hansen, B. J., Edelman, I. S., Solomon, A. K. & Moore, F. D. (1950). J. clin. Invest. 29, 1296.CrossRefGoogle Scholar
Till, A. R. & Downes, A. M. (1962). Aust. J. agric. Res. 13, 335.CrossRefGoogle Scholar
Turner, M. D., Neely, W. A. & Hardy, J. D. (1960). J. appl. Physiol. 15, 309.CrossRefGoogle Scholar