Skip to main content Accessibility help
×
Home

Supplementation of xanthophylls increased antioxidant capacity and decreased lipid peroxidation in hens and chicks

  • Yu-Yun Gao (a1), Qing-Mei Xie (a1), Jing-Yun Ma (a1), Xiang-Bin Zhang (a1), Ji-Mei Zhu (a1), Ding-Ming Shu (a2), Bao-Li Sun (a1), Ling Jin (a1) and Ying-Zuo Bi (a1) (a3)...

Abstract

The present study investigated the effects of xanthophyll supplementation on production performance, antioxidant capacity (measured by glutathione peroxidase, superoxide dismutase (SOD), catalase, total antioxidant capacity (T-AOC), and reduced glutathione:oxidised glutathione ratio (GSH:GSSG)) and lipid peroxidation (measured by malondialdehyde (MDA)) in breeding hens and chicks. In Expt 1, 432 hens were fed diets supplemented with 0 (control group), 20 or 40 mg xanthophyll/kg diet. Blood samples were taken at 7, 14, 21, 28 and 35 d of the trial. Liver and jejunal mucosa were sampled at 35 d. Both xanthophyll groups improved serum SOD at 21 and 28 d, serum T-AOC at 21 d and liver T-AOC, and serum GSH:GSSG at 21, 28 and 35 d and liver GSH:GSSG. Xanthophylls also decreased serum MDA at 21 d in hens. Expt 2 was a 2 × 2 factorial design. Male chicks hatched from 0 or 40 mg in ovo xanthophyll/kg diet of hens were fed a diet containing either 0 or 40 mg xanthophyll/kg diet. Liver samples were collected at 0, 7, 14 and 21 d after hatching. Blood samples were also collected at 21 d. In ovo-deposited xanthophylls increased antioxidant capacity and decreased MDA in the liver mainly within 1 week after hatching. Maternal effects gradually vanished during 1–2 weeks after hatching. Dietary xanthophylls increased antioxidant capacity and decreased MDA in the liver and serum mainly from 2 weeks onwards. Data suggested that xanthophyll supplementation enhanced antioxidant capacity and reduced lipid peroxidation in different tissues of hens and chicks.

Copyright

Corresponding author

*Corresponding author: Professor Y.-Z. Bi, fax +86 20 8528 0283, email yingzuobi@163.com

References

Hide All
1Demmig-Adams, B & Adams, WW III (2002) Antioxidants in photosynthesis and human nutrition. Science 298, 21492153.
2Alves-Rodrigues, A & Shao, A (2004) The science behind lutein. Toxicol Lett 150, 5783.
3Jin, XH, Ohgami, K, Shiratori, K, et al. (2006) Inhibitory effects of lutein on endotoxin-induced uveitis in Lewis rats. Invest Ophthalmol Vis Sci 47, 25622568.
4Rafi, MM & Shafaie, Y (2007) Dietary lutein modulates inducible nitric oxide synthase (iNOS) gene and protein expression in mouse macrophage cells (RAW 264.7). Mol Nutr Food Res 51, 333340.
5Nakagawa, K, Kiko, T, Hatade, K, et al. (2009) Antioxidant effect of lutein towards phospholipid hydroperoxidation in human erythrocytes. Br J Nutr 102, 12801284.
6Serpeloni, JM, Grotto, D, Mercadante, AZ, et al. (2010) Lutein improves antioxidant defense in vivo and protects against DNA damage and chromosome instability induced by cisplatin. Arch Toxicol 84, 811822.
7Lee, EH, Faulhaber, D, Hanson, KM, et al. (2004) Dietary lutein reduces ultraviolet radiation-induced inflammation and immunosuppression. J Invest Dermatol 122, 510517.
8Palombo, P, Fabrizi, G, Ruocco, V, et al. (2007) Beneficial long-term effects of combined oral/topical antioxidant treatment with the carotenoids lutein and zeaxanthin on human skin: a double-blind, placebo-controlled study. Skin Pharmacol Physiol 20, 199210.
9Gonzalez, S, Astner, S, An, W, et al. (2003) Dietary lutein/zeaxanthin decreases ultraviolet B-induced epidermal hyperproliferation and acute inflammation in hairless mice. J Invest Dermatol 121, 399405.
10Dixon, ZR, Burri, BJ, Clifford, A, et al. (1994) Effects of a carotene-deficient diet on measures of oxidative susceptibility and superoxide dismutase activity in adult women. Free Radic Biol Med 17, 537544.
11Bhuvaneswari, V, Velmurugan, B, Balasenthil, S, et al. (2001) Chemopreventive efficacy of lycopene on 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis. Fitoterapia 72, 865874.
12Matos, HR, Capelozzi, VL, Gomes, OF, et al. (2001) Lycopene inhibits DNA damage and liver necrosis in rats treated with ferric nitrilotriacetate. Arch Biochem Biophys 396, 171177.
13Palozza, P, Calviello, G, Emilia De Leo, M, et al. (2000) Canthaxanthin supplementation alters antioxidant enzymes and iron concentration in liver of Balb/c mice. J Nutr 130, 13031308.
14Dixon, ZR, Shie, FS, Warden, BA, et al. (1998) The effect of a low carotenoid diet on malondialdehyde-thiobarbituric acid (MDA-TBA) concentrations in women: a placebo-controlled double-blind study. J Am Coll Nutr 17, 5458.
15Zhao, X, Aldini, G, Johnson, EJ, et al. (2006) Modification of lymphocyte DNA damage by carotenoid supplementation in postmenopausal women. Am J Clin Nutr 83, 163169.
16Sujak, A, Gabrielska, J, Grudzinski, W, et al. (1999) Lutein and zeaxanthin as protectors of lipid membranes against oxidative damage: the structural aspects. Arch Biochem Biophys 371, 301307.
17Woodall, AA, Britton, G & Jackson, MJ (1997) Carotenoids and protection of phospholipids in solution or in liposomes against oxidation by peroxyl radicals: relationship between carotenoid structure and protective ability. Biochim Biophys Acta 1336, 575586.
18Fuhrman, B, Volkova, N, Rosenblat, M, et al. (2000) Lycopene synergistically inhibits LDL oxidation in combination with vitamin E, glabridin, rosmarinic acid, carnosic acid, or garlic. Antioxid Redox Signal 2, 491506.
19Kozuki, Y, Miura, Y & Yagasaki, K (2000) Inhibitory effects of carotenoids on the invasion of rat ascites hepatoma cells in culture. Cancer Lett 151, 111115.
20Kiokias, S & Gordon, MH (2003) Dietary supplementation with a natural carotenoid mixture decreases oxidative stress. Eur J Clin Nutr 57, 11351140.
21Speake, BK, Murray, AM & Noble, RC (1998) Transport and transformations of yolk lipids during development of the avian embryo. Prog Lipid Res 37, 132.
22Ganguly, J, Mehl, JW & Deuel, HJ Jr (1953) Studies on carotenoid metabolism. XII. The effect of dietary carotenoids on the carotenoid distribution in the tissues of chickens. J Nutr 50, 5972.
23Gao, YY, Xie, QM, Jin, L, et al. (2012) Supplementation of xanthophylls decreased proinflammatory and increased anti-inflammatory cytokines in hens and chicks. Br J Nutr (epublication ahead of print version 25 January 2012).
24Koutsos, EA, Lopez, JCG & Klasing, KC (2006) Carotenoids from in ovo or dietary sources blunt systemic indices of the inflammatory response in growing chicks (Gallus gallus domesticus). J Nutr 136, 10271031.
25Karadas, F, Pappas, AC, Surai, PF, et al. (2005) Embryonic development within carotenoid-enriched eggs influences the post-hatch carotenoid status of the chicken. Comp Biochem Physiol B Biochem Mol Biol 141, 244251.
26Surai, PF & Speake, BK (1998) Distribution of carotenoids from the yolk to the tissues of the chick embryo. J Nutr Biochem 9, 645651.
27Gao, YY, Jiang, ZY, Lin, YC, et al. (2011) Effects of spray-dried animal plasma on serous and intestinal redox status and cytokines of neonatal piglets. J Anim Sci 89, 150157.
28Sun, DQ, Li, AW, Li, J, et al. (2009) Changes of lipid peroxidation in carbon disulfide-treated rat nerve tissues and serum. Chem Biol Interact 179, 110117.
29Mares-Perlman, JA, Millen, AE, Ficek, TL, et al. (2002) The body of evidence to support a protective role for lutein and zeaxanthin in delaying chronic disease. Overview. J Nutr 132, 518S524S.
30Chew, BP & Park, JS (2004) Carotenoid action on the immune response. J Nutr 134, 257S261S.
31Sangeetha, RK & Baskaran, V (2010) Retinol-deficient rats can convert a pharmacological dose of astaxanthin to retinol: antioxidant potential of astaxanthin, lutein, and beta-carotene. Can J Physiol Pharmacol 88, 977985.
32He, Y, Root, MM, Parker, RS, et al. (1997) Effects of carotenoid-rich food extracts on the development of preneoplastic lesions in rat liver and on in vivo and in vitro antioxidant status. Nutr Cancer 27, 238244.
33Haddad, JJ (2004) Oxygen sensing and oxidant/redox-related pathways. Biochem Biophys Res Commun 316, 969977.
34Morel, Y & Barouki, R (1999) Repression of gene expression by oxidative stress. Biochem J 342, 481496.
35Surai, PF & Sparks, NH (2001) Comparative evaluation of the effect of two maternal diets on fatty acids, vitamin E and carotenoids in the chick embryo. Br Poult Sci 42, 252259.
36Ojima, F, Sakamoto, H, Ishiguro, Y, et al. (1993) Consumption of carotenoids in photosensitized oxidation of human plasma and plasma low-density lipoprotein. Free Radic Biol Med 15, 377384.
37Surai, AP, Surai, PF, Steinberg, W, et al. (2003) Effect of canthaxanthin content of the maternal diet on the antioxidant system of the developing chick. Br Poult Sci 44, 612619.
38Hohtola, E & Visser, GH (1998) Development of locomotion and endothermy in altricial and precocial birds. In Avian Growth and Development: Evolution within the Altricial – Precocial Spectrum, pp. 157173 [Starck, JM and Ricklefs, RE, editors]. Oxford: Oxford University Press.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed