Skip to main content Accessibility help
×
Home

The role of glutathione S-transferase M1 and T1 gene polymorphisms and fruit and vegetable consumption in antioxidant parameters in healthy subjects

  • Lin-Hong Yuan (a1) (a2), Li-Ping Meng (a3), Wei-Wei Ma (a1), Sheng Li (a1), Jin-Fang Feng (a1), Huan-Ling Yu (a1) and Rong Xiao (a1) (a2)...

Abstract

The correlation of glutathione S-transferase (GST) M1/T1 genetic polymorphisms with oxidative stress-related chronic diseases was proved recently. The aim of the present study was to investigate the association of GSTM1/T1 genetic polymorphisms with antioxidant biomarkers and consumption of fruits and vegetables (F&V) in healthy subjects. In this study, for conducting a 3 d dietary survey, 190 healthy adults were recruited. After DNA extraction, a multiple PCR method was used for GSTM1/T1 genotyping. A spectrophotometer method was applied for the determination of plasma total antioxidant capacity (T-AOC), vitamin C level and erythrocyte GST enzyme activity. A general linear model was used to compare the mean values of antioxidant parameters for different GSTM1/T1 genotypes and consumption of F&V. Polymorphisms of GSTM1/T1 had no effects on plasma T-AOC and vitamin C levels. Deletion of the GSTM1 gene decreased the erythrocyte GST activity. There was correlation between plasma T-AOC and consumption of F&V in the GSTM1 or GSTT1+ subjects. A similar pattern was evident for erythrocyte GST activity in the GSTM1 subjects. No association was found among consumption of F&V and GSTM1/T1 genotypes and plasma vitamin C level. Different consumption of F&V had no impact on plasma T-AOC and vitamin C levels in the GSTM1/GSTT1+ or GSTM1/GSTT1 subjects. The erythrocyte GST activity was more sensitive to consumption of F&V in the individuals with the GSTM1/GSTT1+ genotype. Association was found among GSTM1/T1 genotypes, antioxidant parameters and consumption of F&V. Large-scale and multiple ethnic studies are needed to further evaluate the relationship.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      The role of glutathione S-transferase M1 and T1 gene polymorphisms and fruit and vegetable consumption in antioxidant parameters in healthy subjects
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      The role of glutathione S-transferase M1 and T1 gene polymorphisms and fruit and vegetable consumption in antioxidant parameters in healthy subjects
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      The role of glutathione S-transferase M1 and T1 gene polymorphisms and fruit and vegetable consumption in antioxidant parameters in healthy subjects
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: R. Xiao, fax +86 010 83911512, email xiaor22@ccmu.edu.cn

References

Hide All
1 Hayes, JD & Strange, RC (2000) Glutathione S-transferase polymorphisms and their biological consequences. Pharmacology 61, 154166.
2 Strange, RC, Jones, PW & Fryer, AA (2000) Glutathione S-transferase: genetics and role in toxicology. Toxicol Lett 112–113, 357363.
3 Brind, AM, Hurlstone, A, Edrisinghe, D, et al. (2004) The role of polymorphism of glutathines S-transferases GSTM1, M3, P1, T1 and A1 in susceptibility to alcoholic liver disease. Alcohol Alcohol 39, 478483.
4 Katoh, T, Kaneko, S, Takasawa, S, et al. (1999) Human glutathione S-transferase P1 polymorphism and susceptibility to smoking related epithelial cancer; oral, lung, gastric, colorectal and urothelial cancer. Pharmacogenetics 9, 165169.
5 Chan, EC, Lam, SY, Fu, KH, et al. (2005) Polymorphisms of the GSTM1, GSTP1, MPO, XRCC1, and NQO1 genes in Chinese patients with non-small cell lung cancers: relationship with aberrant promoter methylation of the CDKN2A and RARB genes. Cancer Genet Cytogenet 162, 1020.
6 Curran, JE, Weinstein, SR & Griffiths, LR (2000) Polymorphisms of glutathione S-transferase genes (GSTM1, GSTP1 and GSTT1) and breast cancer susceptibility. Cancer Lett 153, 113120.
7 Casson, AG, Zheng, ZY, Geoffrey, AP, et al. (2006) Genetic polymorphisms of microsomal epoxide hydroxylase and glutathione S-transferases M1, T1 and P1, interactions with smoking, and risk for esophageal (Barrett) adenocarcinoma. Cancer Detect Prev 30, 423431.
8 Binkova, B, Chvatalova, I, Lnenickova, Z, et al. (2007) PAH–DNA adducts in environmentally exposed population in relation to metabolic and DNA repair gene polymorphisms. Mutat Res 620, 4961.
9 Masetti, S, Botto, N, Manfredi, S, et al. (2003) Interactive effect of the glutathione S-transferase genes and cigarette smoking on occurrence and severity of coronary artery risk. J Mol Med 81, 488489.
10 Tamer, L, Ercan, B, Camsari, A, et al. (2004) Glutathione S-transferase gene polymorphism as a susceptibility factor in smoking-related coronary artery disease. Basic Res Cardiol 99, 223229.
11 Girisha, KM, Gilmour, A, Mastana, S, et al. (2004) T1 and M1 polymorphism in glutathione S-transferase gene and coronary artery disease in North Indian population. Indian J Med Sci 58, 520526.
12 Strain, JJ, Elwood, PC, Davis, A, et al. (2000) Frequency of fruit and vegetable consumption and blood antioxidants in the Caerphilly cohort of older men. Eur J Clin Nutr 54, 828833.
13 Park, EJ & Pezzuto, JM (2002) Botanicals in cancer chemoprevention. Cancer Metastasis Rev 21, 231255.
14 Chen, C & Kong, AN (2004) Dietary chemopreventive compounds and ARE/EpRE signaling. Free Radic Biol Med 36, 15051516.
15 Omenn, GS, Goodman, GE, Thornquist, MD, et al. (1996) Risk factors for lung cancer and for intervention effects in CARET, the β-carotene and retinol efficacy trial. J Natl Cancer Inst 88, 15501559.
16 Zhong, S, Wyllie, AH, Barnes, D, et al. (1993) Relationship between the GSTM1 genetic polymorphism and susceptibility to bladder, breast and colon cancer. Carcinogenesis 14, 18211824.
17 Zhong, SL, Zhou, S, Chen, X, et al. (2006) Rapid determination of common mutation in glutathione S-transferase gene by PCR-based methods in healthy Chinese. Clin Chim Acta 364, 205208.
18 Jacques-Silva, MC, Nogueira, CW, Broch, LC, et al. (2001) Diphenyl diselenide and ascorbic acid changes deposition of selenium and ascorbic acid in liver and brain of mice. Pharmacol Toxicol 88, 119125.
19 Habig, WH & Jakoby, WB (1981) Assays for differentiation of glutathione S-transferases. Methods Enzymol 77, 398405.
20 Min, JW, Park, H, Park, B, et al. (2006) Paraoxonase gene polymorphism and vitamin levels during pregnancy: relationship with maternal oxidative stress and neonatal birthweights. Reprod Toxicol 22, 418424.
21 Agachan, B, Yilmaz, H, Ergen, HA, et al. (2005) Paraoxonase (PON1) 55 and 192 polymorphism and its effects to oxidant-antioxidant system in Turkish patient with type 2 diabetes mellitus. Physiol Res 54, 287293.
22 Zhong, SL, Zhou, SF, Chen, X, et al. (2006) Relationship between genotype and enzyme activity of glutathione S-transferases M1 and P1 in Chinese. Eur J Pharm Sci 28, 7784.
23 Tang, JJ, Wang, MW, Jia, EZ, et al. (2010) The common variant in the GSTM1 and GSTT1 genes is related to markers of oxidative stress and inflammation in patients with coronary artery disease: a case-only study. Mol Biol Rep 37, 405410.
24 Mustafa, MD, Pathak, R, Ahmed, T, et al. (2010) Association of glutathione S-transferase M1 and T1 gene polymorphisms and oxidative stress markers in preterm labor. Clin Biochem 43, 11241128.
25 Martin, NJ, Collier, AC, Bowen, LD, et al. (2009) Polymorphisms in the NQO1, GSTT and GSTM genes are associated with coronary heart disease and biomarkers of oxidative stress. Mutat Res 674, 93100.
26 Dušinská, M, Ficek, A, Horská, A, et al. (2001) Glutathione S-transferase polymorphisms influence the level of oxidative DNA damage and antioxidant protection in humans. Mutat Res 482, 4755.
27 Wilms, LC, Claughton, TA, De Kok, TM, et al. (2007) GSTM1 and GSTT1 polymorphism influences protection against induced oxidative DNA damage by quercetin and ascorbic acid in human lymphocytes in vitro. Food Chem Toxicol 45, 25922596.
28 Tijhuis, MJ, Visker, MH, Aarts, JM, et al. (2007) Glutathione S-transferase phenotypes in relation to genetic variation and fruit and vegetable consumption in an endoscopy-based population. Carcinogensis 28, 848857.
29 Nijhoff, WA, Mulder, TPJ, Verhagen, H, et al. (1995) Effects of consumption of brussels sprouts on plasma and urinary glutathione S-transferase class-alpha and -pi in humans. Carcinogenesis 16, 955957.
30 Lampe, JW, Chen, C, Li, S, et al. (2000) Modulation of human glutathione S-transferases by botanically defined vegetable diets. Cancer Epidemiol Biomarkers Prev 9, 787793.

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed