Skip to main content Accessibility help
×
Home

Response of ApoA-IV in pigs to long-term increased dietary oil intake and to the degree of unsaturation of the fatty acids

  • María A. Navarro (a1), Sergio Acín (a1), Ricardo Carnicer (a1), Mario A. Guzmán-García (a1), José M. Arbonés-Mainar (a1), Joaquín C. Surra (a1), José A. Cebrián (a1), Carmen Arnal (a2), Beatriz Isabel (a3), Clemente J. López-Bote (a3) and Jesús Osada (a1)...

Abstract

ApoA-IV is a protein constituent of HDL particles; the gene coding for it is a member of the ApoA-I–ApoC-III–ApoA-IV cluster. To investigate the effects of the quantity and the degree of saturation of dietary lipid on the long-term response of this Apo, and on the hypothetical coordinated regulation of the cluster in vivo, pigs were fed isoenergetic, cholesterol-free, low-lipid or lipid-enriched diets (containing either extra olive oil (rich in MUFA) or sunflower oil (rich in n−6 PUFA)) for 42 d. In animals fed on the control diet, ApoA-IV was mainly associated with plasma lipoproteins. An increase in plasma ApoA-IV concentration, mainly in the lipoprotein-free fraction, was induced by the lipid-enriched diets, independent of the degree of saturation of the fatty acids involved. The latter diets also led to increases in hepatic ApoA-I, ApoA-IV and ApoC-III mRNA levels, more so with the sunflower oil-rich diet. The present results show that porcine plasma ApoA-IV levels and their association with lipoproteins are very sensitive to increases in dietary lipids, independent of the degree of fatty acid saturation. Furthermore, hepatic expression of RNA appears to be coordinated along with that of the other members of the gene cluster.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Response of ApoA-IV in pigs to long-term increased dietary oil intake and to the degree of unsaturation of the fatty acids
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Response of ApoA-IV in pigs to long-term increased dietary oil intake and to the degree of unsaturation of the fatty acids
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Response of ApoA-IV in pigs to long-term increased dietary oil intake and to the degree of unsaturation of the fatty acids
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Dr J. Osada, fax +34 976 761 612, email, Josada@unizar.es

References

Hide All
Aalto-Setälä, K, Bisgaier, CL, Ho, A, Kiett, KA, Traber, MG, Kayden, MJ, Kamakrishnan, R, Walsh, A, Essendurbg, AD & Breslow, JL (1994) Intestinal expression of apolipoprotein A-IV in transgenic mice fails to influence dietary lipid absortion or feeding behavior. J Clin Invest 93, 17761786.
Assman, G & Nofer, JR (2003) Atheroprotective effects of high density lipoproteins. Annu Rev Med 54, 321341.
Baroukh, N, Ostos, MA, Vergnes, L, Recalde, D, Staels, B, Fruchart, J, Ochoa, A, Castro, G & Zakin, MM (2001) Expression of human apolipoprotein A-I/C-III/A-IV gene cluster in mice reduces atherogenesis in response to a high fat-high cholesterol diet. FEBS Lett 50, 1620.
Bisgaier, CL, Sachdev, OP, Megna, L & Glickman, RM (1985) Distribution of apolipoprotein A-IV in human plasma. J Lipid Res 26, 1125.
Black, DD & Rohwer-Nutter, PL (1991) Intestinal apolipoprotein synthesis in the new born piglet. Pediatr Res 29, 3238.
Black, DD, Wang, H, Hunter, F & Zhan, R (1996) Intestinal expression of apolipoprotein A-IV and C-III is coordinately regulated by dietary lipid in newborn swine. Biochem Biophys Res Commun 22, 619624.
Brousseau, ME, Stucchi, AF, Vespa, DB, Schaefer, EJ & Nicolosi, RJ (1993) A diet enriched in monounsaturated fats decreases low density lipoprotein concentrations in Cynomolgus monkeys by a different mechanism than does a diet enriched in polyunsaturated fats. J Nutr 123, 20492058.
Calleja, L, Paris, MA, Paul, A, Vilella, E, Joven, J, Jimenez, A, Beltran, G, Uceda, M, Maeda, N & Osada, J (1999) Low-cholesterol and high-fat diets reduce atherosclerotic lesion development in ApoE-knockout mice. Arterioscler Thromb Vasc Biol 19, 23682375.
Calleja, L, Trallero, MC, Carrizosa, C, Mendez, MT, Palacios-Alaiz, E & Osada, J (2000) Effects of dietary fat amount and saturation on the regulation of hepatic mRNA and plasma apolipoprotein A-I in rats. Atherosclerosis 15, 6978.
DeLamatre, JG, Hoffmeier, CA, Lacko, AG & Roheim, PS (1983) Distribution of apolipoprotein A-IV between the lipoprotein and the lipoprotein-free fractions of rat plasma: possible role of lecitin:cholesterol acyltransferase. J Lipid Res 24, 15781585.
De Silva, HS, Mas-Oliva, J, Taylor, JM & Mahley, RW (1994) Identification of apolipoprotein B-100 low density lipoproteins, apoB-48 remnants, and apolipoprotein E-rich high density lipoproteins in the mouse. J Lipid Res 35, 12971310.
Dreon, DM, Fernstrom, HA, Campos, H, Blanche, P, Williams, PT & Krauss, RM (1998) Change in dietary saturated fat intake is correlated with change in mass of large low-density-lipoprotein particles in men. Am J Clin Nutr 67, 828836.
Duverger, N, Tremp, G, Caillaud, JM, Emmanuel, F, Castro, G, Fruchart, JC, Steinmetz, A & Denefle, P (1996) Protection against atherogenesis in mice mediated by human apolipoprotein A-IV. Science 27, 966968.
Kalogeris, TJ, Monroe, F, Demichele, SJ & Tso, P (1996) Intestinal synthesis and lymphatic secretion of apolipoprotein A-IV vary with chain length of intestinally infused fatty acids in rats. J Nutr 12, 27202729.
Karathanasis, SK (1985) Apolipoprotein multigene family: tandem organization of human apolipoprotein A-I, C-III and A-IV genes. Proc Natl Acad Sci USA 82, 63746378.
Karathanasis, SK, Yunis, I & Zannis, VI (1986) Structure, evolution, and tissue-specific synthesis of human apolipoprotein AIV. Biochemistry 25, 39623970.
Kratz, M, Wahrburg, U, von Eckardstein, A, Ezeh, B, Assmann, G & Kronenberg, F (2003) Dietary mono- and polyunsaturated fatty acids similarly increase plasma apolipoprotein A-IV concentrations in healthy men and women. J Nutr 13, 18211825.
Lagrost, L, Gambert, P, Boquillon, M & Lallemant, C (1989) Evidence for high density lipoproteins as the major apolipoprotein A-IV-containing fraction in normal human serum. J Lipid Res 30, 15251534.
LeBoeuf, RC, Caldwell, M & Kirk, E (1994) Regulation by nutritional status of lipids and apolipoproteins A-I, A-II and A-IV in inbred mice. J Lipid Res 35, 121133.
Maeda, N, Ebert, DL, Doers, TM, Newman, M, Hasler-Rapacz, J, Attie, AD, Rapacz, J & Smithies, O (1988) Molecular genetics of the apolipoprotein B gene in pigs in relation to atherosclerosis. Gene 70, 213229.
Mata, P, Garrido, JA, Ordovas, JM, Blazquez, E, Alvarez-Sala, LA, Rubio, MJ, Alonso, R & de Oya, M (1992) Effect of dietary monounsaturated fatty acids on plasma lipoproteins and apolipoproteins in women. Am J Clin Nutr 56, 7783.
Mattson, FH & Grundy, SM (1985) Comparison of effects of dietary saturated, monounsaturated, and polyunsaturated fatty acids on plasma lipids and lipoproteins in man. J Lipid Res 26, 194202.
Moghadasian, MH (2002) Experimental atherosclerosis: a historical overview. Life Sci 70, 855865.
Mustad, VA, Ellsworth, JL, Cooper, AD, KrisEtherton, PM & Etherton, TD (1996) Dietary linoleic acid increases and palmitic acid decreases hepatic LDL receptor protein and mRNA abundance in young pigs. J Lipid Res 37, 23102323.
Navarro, MA, Acín, S & Iturralde, M et al. (2004) Cloning, characterization and comparative analysis of pig plasma apolipoprotein A-IV. Gene 32, 157164.
Osada, J, Fernandez-Sanchez, A, Diaz-Morillo, JL, Miró-Obradors, MJ, Cebrián, JA, Carrizosa, C, Ordovás, J & Palacios-Alaiz, E (1994) Differential effect of dietary fat saturation and cholesterol on hepatic apolipoprotein gene expression in rats. Atherosclerosis 10, 8390.
Osada, J & Maeda, N (1998) Preparation of knockout mice. Methods Mol Biol 11, 7992.
Pussinen, PJ, Jauhiainen, M & Ehnholm, C (1997) ApoA-II/apoA-I molar ratio in the HDL particle influences phospholipid transfer protein-mediated HDL interconversion. J Lipid Res 38, 1221.
Rudel, LL, Haines, JL & Sawyer, JK (1990) Effects on plasma lipoproteins of monounsaturated, saturated and polyunsaturated fatty acids in the diet of African green monkeys. J Lipid Res 31, 18731882.
Sloop, CH, Dory, L, Hamilton, R, Krause, BR & Roheim, PS (1983) Characterization of dog peripheral lymph lipoproteins: the presence of a disc shaped “Nascent” high density lipoprotein. J Lipid Res 24, 14291440.
Spady, DK, Kearney, DM & Hobbs, HH (1999) Polyunsaturated fatty acids up-regulate hepatic scavenger receptor B1 (SR-BI) expression and HDL cholesteryl ester uptake in the hamster. J Lipid Res 40, 13841394.
Stan, S, Delvin, E, Lambert, M, Seidman, E & Levy, E (2003) Apo A-IV: an update on regulation and physiologic functions. Biochim Biophys Acta 163, 177187.
Trieu, VN, Hasler-Rapacz, J, Rapacz, J & Black, DD (1993) Sequences and expression of the porcine apolipoprotein A-I and C-III mRNAs. Gene 12, 173179.
van Tol, A, Terpstra, AHM, van den Berg, P & Beynen, AC (1999) Dietary corn oil versus olive oil enhances HDL protein turnover and lowers HDL cholesterol levels in hamsters. Atherosclerosis 147, 8794.
Vergnes, L, Taniguchi, T, Omori, K, Zakin, MM & Ochoa, A (1997) The apolipoprotein A-I/C-III/A-IV gene cluster: Apo C-III and Apo A-IV expression is regulated by two common enhancers. Biochim Biophys Acta 134, 299310.
Wang, H, Zhan, R, Hunter, F, Du, JH & Black, D (1996) Effect of acute feeding of diets of varying fatty acid composition on intestinal apolipoprotein expression in the newborn swine. Pediatr Res 39, 10781084.
Wang, H, Hunter, F & Black, DD (1998) Effect of feeding diets of varying fatty acid composition on apolipoprotein expression in newborn swine. Am J Physiol 38, G645G651.
Weisgraber, KH, Bersot, TP & Mahley, RW (1978) Isolation and characterization of an apolipoprotein from the d less than 1.006 lipoproteins of human and canine lymph homologous with the rat A-IV apolipoprotein. Biochem Biophys Res Commun 85, 287292.

Keywords

Response of ApoA-IV in pigs to long-term increased dietary oil intake and to the degree of unsaturation of the fatty acids

  • María A. Navarro (a1), Sergio Acín (a1), Ricardo Carnicer (a1), Mario A. Guzmán-García (a1), José M. Arbonés-Mainar (a1), Joaquín C. Surra (a1), José A. Cebrián (a1), Carmen Arnal (a2), Beatriz Isabel (a3), Clemente J. López-Bote (a3) and Jesús Osada (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed