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Reduction of dietary glycaemic load modifies the expression of microRNA potentially associated with energy balance and cancer pathways in pre-menopausal women

  • Susan E. McCann (a1), Song Liu (a2), Dan Wang (a2), Jie Shen (a1), Qiang Hu (a2), Chi-Chen Hong (a1), Vicky A. Newman (a3) and Hua Zhao (a1)...

Abstract

Energy metabolism, insulin resistance and adiposity have been implicated in breast cancer, but dietary interventions to reduce breast cancer morbidity and mortality have had limited success. MicroRNA (miRNA) are short, non-coding RNA that participate in the control of metabolic processes through the post-transcriptional modification of RNA. We investigated the effect of a low-glycaemic load dietary intervention on miRNA expression, with subsequent bioinformatics pathway analyses to explore metabolic pathways potentially affected by the diet. Total RNA, including miRNA, was isolated from the serum of fourteen otherwise healthy pre-menopausal women with a high breast cancer risk participating in a 12-month dietary intervention designed to lower glycaemic load by at least 15 % from baseline. Genome-wide miRNA expression was conducted using Illumina BeadChips. In the intervention subjects, three differentially expressed miRNA were validated by real-time (RT)-PCR, and in the twenty control participants, four top differentially expressed miRNA were evaluated to confirm a diet effect. In post-intervention v. baseline serum, twenty miRNA were found to be differentially expressed, with twelve up-regulated and eight down-regulated. These differentially expressed miRNA were predicted to be potentially associated with energy balance and cancer pathways based on exploratory enrichment analysis. Quantitative RT-PCR validations in the controls confirmed that the observed miRNA differential expression was dietary intervention induced. Manipulation of dietary glycaemic load has the potential to modify the expression of multiple miRNA predicted to be involved in energy balance and cancer pathways. Further research is necessary to confirm the role of these miRNA in the control of energy metabolism and relationships with cancer-related processes.

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Corresponding author

*Corresponding authors: Dr S. E. McCann, E-mail: susan.mccann@roswellpark.org; Dr S. Liu, E-mail: song.liu@roswellpark.org

References

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1Patterson, RE, Cadmus, LA, Emond, JA, et al. (2010) Physical activity, diet, adiposity and female breast cancer prognosis: a review of the epidemiologic literature. Maturitas 66, 515.
2Harvie, M & Howell, A (2006) Energy balance adiposity and breast cancer – energy restriction strategies for breast cancer prevention. Obes Rev 7, 3347.
3Kaaks, R & Lukanova, A (2001) Energy balance and cancer: the role of insulin and insulin-like growth factor-I. Proc Nutr Soc 60, 91106.
4McTiernan, A (2005) Obesity and cancer: the risks, science, and potential management strategies. Oncology (Williston Park) 19, 871881.
5Marshall, S (2006) Role of insulin, adipocyte hormones, and nutrient-sensing pathways in regulating fuel metabolism and energy homeostasis: a nutritional perspective of diabetes, obesity, and cancer. Sci STKE 2006, re7.
6Bartel, DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116, 281297.
7Davis, CD & Ross, SA (2008) Evidence for dietary regulation of microRNA expression in cancer cells. Nutr Rev 66, 477482.
8Poy, MN, Spranger, M & Stoffel, M (2007) MicroRNAs and the regulation of glucose and lipid metabolism. Diabetes Obes Metab 9, Suppl. 2, 6773.
9Xie, H, Sun, L & Lodish, HF (2009) Targeting microRNAs in obesity. Expert Opin Ther Targets 13, 12271238.
10Zhang, J, Zhang, F, Didelot, X, et al. (2009) Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring. BMC Genomics 10, 478.
11Baek, D, Villen, J, Shin, C, et al. (2008) The impact of microRNAs on protein output. Nature 455, 6471.
12Selbach, M, Schwanhausser, B, Thierfelder, N, et al. (2008) Widespread changes in protein synthesis induced by microRNAs. Nature 455, 5863.
13Gauthier, BR & Wollheim, CB (2006) MicroRNAs: ‘ribo-regulators’ of glucose homeostasis. Nat Med 12, 3638.
14Kendall, CW, Augustin, LS, Emam, A, et al. (2006) The glycemic index: methodology and use. Nestle Nutr Workshop Ser Clin Perform Programme 11, 4353.
15Jahr, H, Schroder, D, Ziegler, B, et al. (1980) Transcriptional and translational control of glucose-stimulated (pro)insulin biosynthesis. Eur J Biochem 110, 499505.
16Lee, EK & Gorospe, M (2010) Minireview: posttranscriptional regulation of the insulin and insulin-like growth factor systems. Endocrinology 151, 14031408.
17Boyd, NF, Byng, JW, Jong, RA, et al. (1995) Quantitative classification of mammographic densities and breast cancer risk: results from the Canadian National Breast Screening Study. J Natl Cancer Inst 87, 670675.
18Boyd, NF, Lockwood, GA, Byng, JW, et al. (1998) Mammographic densities and breast cancer risk. Cancer Epidemiol Biomarkers Prev 7, 11331144.
19Smyth, GK (2004) Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol 3, R3.
20Benjamini, Y & Hochberg, Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. J Royal Stat Soc Series B 57, 289300.
21Eisen, MB, Spellman, PT, Brown, PO, et al. (1998) Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci U S A 95, 1486314868.
22Friedman, RC, Farh, KK, Burge, CB, et al. (2009) Most mammalian mRNAs are conserved targets of microRNAs. Genome Res 19, 92105.
23Betel, D, Wilson, M, Gabow, A, et al. (2008) The microRNA.org resource: targets and expression. Nucleic Acids Res 2008 36, D149D153(database issue).
24Miranda, KC, Huynh, T, Tay, Y, et al. (2006) A pattern-based method for the identification of microRNA binding sites and their corresponding heteroduplexes. Cell 126, 12031217.
25Wang, X (2008) miRDB: a microRNA target prediction and functional annotation database with a wiki interface. RNA 14, 10121017.
26Dweep, H, Sticht, C, Pandey, P, et al. (2011) miRWalk – database: prediction of possible miRNA binding sites by “walking” the genes of three genomes. J Biomed Inform 44, 839847.
27Sethupathy, P, Megraw, M & Hatzigeorgiou, AG (2006) A guide through present computational approaches for the identification of mammalian microRNA targets. Nat Methods 3, 881886.
28Huang, W, Sherman, BT, Tan, Q, et al. (2007) DAVID bioinformatics resources: expanded annotation database and novel algorithms to better extract biology from large gene lists. Nucleic Acids Res 35, W169W175.
29Doll, R & Peto, R (1981) The causes of cancer: quantitative estimates of avoidable risks of cancer in the United States today. J Natl Cancer Inst 66, 11911308.
30World Cancer Research Fund (1997) Food, Nutrition and the Prevention of Cancer: a Global Perspective. Washington, DC: American Institute for Cancer Research.
31World Cancer Research Fund/American Institute for Cancer Research (2007) Food, Nutrition, Physical Activity, and the Prevention of Cancer: a Global Perspective. Washington, DC: AICR.
32Finley, CE, Barlow, CE, Halton, TL, et al. (2010) Glycemic index, glycemic load, and prevalence of the metabolic syndrome in the cooper center longitudinal study. J Am Diet Assoc 110, 18201829.
33Fair, AM, Dai, Q, Shu, XO, et al. (2007) Energy balance, insulin resistance biomarkers, and breast cancer risk. Cancer Detect Prev 31, 214219.
34Linos, E & Willett, WC (2007) Diet and breast cancer risk reduction. J Natl Compr Canc Netw 5, 711718.
35Pan, SY & DesMeules, M (2009) Energy intake, physical activity, energy balance, and cancer: epidemiologic evidence. Methods Mol Biol 472, 191215.
36Saxton, JM (2006) Diet, physical activity and energy balance and their impact on breast and prostate cancers. Nutr Res Rev 19, 197215.
37Zhao, Y, Deng, C, Wang, J, et al. (2011) Let-7 family miRNAs regulate estrogen receptor alpha signaling in estrogen receptor positive breast cancer. Breast Cancer Res Treat 127, 6980.
38Yu, F, Yao, H, Zhu, P, et al. (2007) Let-7 regulates self renewal and tumorigenicity of breast cancer cells. Cell 131, 11091123.
39O'Day, E & Lal, A (2010) MicroRNAs and their target gene networks in breast cancer. Breast Cancer Res 12, 201.
40Zhang, B, Pan, X, Cobb, GP, et al. (2007) MicroRNAs as oncogenes and tumor suppressors. Dev Biol 302, 112.
41Greene, SB, Herschkowitz, JI & Rosen, JM (2010) The ups and downs of miR-205: identifying the roles of miR-205 in mammary gland development and breast cancer. RNA Biol 7, 300304.
42Wu, H & Mo, YY (2009) Targeting miR-205 in breast cancer. Expert Opin Ther Targets 13, 14391448.
43Tili, E, Michaille, JJ, Alder, H, et al. (2010) Resveratrol modulates the levels of microRNAs targeting genes encoding tumor-suppressors and effectors of TGFbeta signaling pathway in SW480 cells. Biochem Pharmacol 80, 20572065.
44Tili, E, Michaille, JJ, Adair, B, et al. (2010) Resveratrol decreases the levels of miR-155 by upregulating miR-663, a microRNA targeting JunB and JunD. Carcinogenesis 31, 15611566.
45Lehmann, U, Hasemeier, B, Christgen, M, et al. (2008) Epigenetic inactivation of microRNA gene hsa-mir-9-1 in human breast cancer. J Pathol 214, 1724.

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Reduction of dietary glycaemic load modifies the expression of microRNA potentially associated with energy balance and cancer pathways in pre-menopausal women

  • Susan E. McCann (a1), Song Liu (a2), Dan Wang (a2), Jie Shen (a1), Qiang Hu (a2), Chi-Chen Hong (a1), Vicky A. Newman (a3) and Hua Zhao (a1)...

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