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Polyamine and intestinal properties in adult rats

  • Patricia Deloyer (a1), Guy Dandrifosse (a1), Catherine Bartholomeus (a1), Nadine Romain (a1), Monique Klimek (a1), JosÉ Salmon (a1), Paul Gérard (a2), Guy Goessens (a3) and Hendrik Eyssen (a4)...

Abstract

We questioned whether polyamines coming from the diet or produced by intestinal microflora or by intracellular metabolism influence intestinal functions. Therefore, we compared pathogen-free rats and germ-free rats receiving a diet with low polyamine content and either treated or not treated with difluoromethylornithine (DFMO) and/or methylglyoxal bis (guanylhydrazone) (MGBG). Wet weight, protein content, DNA content, sucrase (EC3.2.1.48), maltase (EC 3.2.1.20) and lactase (EC 3.2.1.23) specific activities, amounts of putrescine, spennidine and spemine were measured in the mucosa of the proximal and distal intestine. Body weight was also determined. Rats without microflora had a higher specific activity of maltase and higher amounts of spermidine and spermine but lower lactase specific activity than pathogen-free animals; the low-polyamine diet given to gem-free rats had little effect on the functional variables measured (decrease of maltase and lactase specific activities) and did not modify the amounts of polyamines. DFMO and/or MGBG administered to germ-free rats receiving a low-polyamine diet induced modifications of most of the variables studied. Body weight and wet weight of proximal and distal intestine decreased, disaccharidase specific activities decreased, and amounts of polyamines changed according to the inhibitor used. Thus, our results showed that the deprivation of polyamine supply from microflora or from the diet failed, under our experimental conditions, to affect the intestinal properties analysed but exogenous and endogenous polyamine restriction altered general properties of the organism as well as intestinal functions.

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References

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Alarcon, P., Lebenthal, E. & Lee, P. C. (1987). Effect of difluoromethyl ornithine (DFMO) on small intestine of adult and weaning rats. Digestive Diseases and Sciences 32, 883888.
Bardócz, S., Brown, D. S., Grant, G. & Pusztai, A. (1990). Luminal and basolateral polyamine uptake by rat small intestine stimulated to grow by Phaseolus vulgaris lectin phytohaemagglutinin in vivo. Biochimica et Biophysica Acta 1034, 4652.
Bardócz, S., Duguid, T. C., Brown, D. S., Grant, G., Pusztai, A., White, A. & Ralph, A. (1995). The importance of dietary polyamines in cell regeneration and growth. British Journal of Nutrition 73, 819828.
Bardócz, S., Grant, G., Brown, D. S., Ewen, S. W. B., Stewart, J. C. & Pusztai, A. (1991). Effect of fasting and refeeding on basolateral polyamine uptake and metabolism by the rat small bowel. Digestion 50, 2835.
Bontemps, J., Laschet, J., Dandrifosse, G., Van Custem, J. L. & Forget, P. (1984). Analysis of dansyl derivatives of di- and polyamines in mouse brain, human serum and duodenal biopsy by high-performance liquid chromatography on a standard reversed-phase column. Journal of Chromatography 311, 5967.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248254.
Brown, N. D. & Strickler, H. P. (1982). Femtomolar ion-pair high-performance liquid chromatographic method for determining Dns-polyamine derivatives of red blood cell extract utilizing an automated polyamine analyser. Journal of Chromatography 295, 101108.
Chauveau, J., Clement, G., Clement-Champougny, F. & Le Breton, E. (1951). Non-equilibrium between the lipid constituents of cellular structures in the course of initiation of experimental hepatoma in the rat cell. The lipids of rat liver fractions. Archives des Sciences Physiologiques 5, 305322.
Dahlqvist, A. (1968). Assay of intestinal disaccharidases. Analytical Biochemistry 22, 99107.
Diamond, J. M. (1986). Hard-wired local triggering of intestinal enzyme expression. Nature 324, 408.
Dufour, C., Dandrifosse, G., Forget, P., Vermesse, F., Romain, N. & Lepoint, A. (1988). Spermine and spermidine induce intestinal maturation in the rat. Gastroenterology 95, 112116.
Forget, P., Lombet, J., Grandfils, C., Dandrifosse, G. & Geubelle, F. (1985). Lactase insufficiency revisited. Journal of Pediatric Gastroenterology and Nutrition 4, 868872.
Georges, P., Dandrifosse, G., Vermesse, F., Forget, P., Deloyer, P. & Romain, N. (1990). Reversibility of spermine-induced intestinal maturation in the rat. Digestive Diseases and Sciences 35, 15281535.
Gordon, J. I. & Hermiston, M. L. (1994). Differentiation and self-renewal in the mouse gastrointestinal epithelium. Current Opinion in Cell Biology 6, 795803.
Hosomi, M., Stace, N., Murphy, G. M. & Dowling, R. H. (1985). Cellular regulation of adaptive intestinal mucosa hyperplasia: effect of the polyamine synthesis blocker DFMO. Gastroenterology 88, 1715.
Ittel, T. H., Paulus, C.-P., Handt, S., Hofstädter, F. & Sieberth, H-G. (1992). Induction of intestinal mucosal atrophy by difluoromethylornithine: a nonuremic model of enhanced aluminium absorption. Mineral and Electrolyte Metabolism 18, 1523.
Jänne, J., Poso, H. & Raina, A. (1978). Polyamines in rapid growth and cancer. Biochimica et Biophysica Acta 473, 241293.
Luk, G. D. & Baylin, S. B. (1983). Polyamines and intestinal growth-increased polyamine biosynthesis after jejunectomy. American Journal of Physiology 245, G656G660.
Luk, G. D., Marton, L. J. & Baylin, S. B. (1980). Ornithine decarboxylase is important in intestinal mucosal maturation and recovery from injury in rat. Science 210, 195198.
Moulinoux, J.-P., Darcel, F., Quemener, V., Havouis, R. & Seiler, N. (1991). Inhibition of the growth of U-251 human glioblastoma in nude mice by polyamine deprivation. Anticancer Research 11, 175180.
Pegg, A. F. & McCann, P. P. (1982). Polyamine metabolism and function. American Journal of Physiology 243, C212C221.
Pollack, P. F., Koldovky, O. & Nishioka, K. (1992). Polyamines in human and rat milk and infant formulas. American Journal of Clinical Nutrition 56, 371375.
Romain, N.Dandrifosse, G., Jeusette, F. & Forget, P. (1992). Polyamine concentration in rat milk and food, human milk and infant formulae. Pediatric Research 32, 5863.
Schneider, W. C. (1957). Determination of nucleic acids in tissues by pentose analysis. In Methods in Enzymology, vol. 3, pp. 680684, [Colowick, S. P. & Kaplan, N. O., editors]. New York: Academic Press.
Yarrington, J. T., Sprinkle, D. J., Loudy, D. E., Diekema, K. A., McCann, P. P. & Gibson, J. P. (1983). Intestinal changes caused by DL-α-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase. Experimental and Molecular Pathology 39, 300316.

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Polyamine and intestinal properties in adult rats

  • Patricia Deloyer (a1), Guy Dandrifosse (a1), Catherine Bartholomeus (a1), Nadine Romain (a1), Monique Klimek (a1), JosÉ Salmon (a1), Paul Gérard (a2), Guy Goessens (a3) and Hendrik Eyssen (a4)...

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