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Physiological zinc-binding proteins of medium molecular weight in the rat gut

  • Malcolm J. Jackson (a1), Daphne Holt (a2), Michael Webb (a2) and Nicholas D. Carter (a3)

Abstract

1. Gel filtration on Sephadex G 75 was used to separate the medium-molecular-weight zinc-binding proteins from the soluble fractions from the duodenal and jejuno-ileal segments of the rat gut at 30 min after the intragastric administration of a tracer dose of 65Zn. These proteins were resolved by ion-exchange chromatography on DEAE cellulose.

2. In both the duodenum and jejuno-ileal segment an appreciable fraction of the total soluble Zn was bound in a protein fraction that resembled metallothionein [MT] in its behaviour on gel filtration. These fractions, however, were not homogeneous, but contained several medium-molecular-weight Zn-binding proteins. In the duodenum, but not in the jejuno-ileal segment, two ofthese proteins appeared to be the isometallothioneins, ZnMT-I and ZnMT-11.

3. These results suggest a possible role for MT in the binding of newly-absorbed Zn in the duodenal mucosal cells. They also show that gel filtration alone is insufficient for the identification of MT in the intestine.

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References

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Bonewitz, R. E., Foulkes, E. C., O'flaherty, E. J. & Hertsberg, V. (1982). In Biological Roles of metallothionein, pp. 203214 [Foulkes, E. C., editor]. New York: Elsevier/North-Holland.
Bremner, I. (1982). In Trace Element Metabolism in Man and Animals, pp. 637644 [Gawthorne, J. M., Howell, J. McC. and White, C. L., editors]. Berlin, Heidelberg and New York: Springer-Verlag.
Bremner, I. & Davies, N. J. (1975). Biochemical Journal 149, 733738.
Cousins, R. J. (1979). Nutrition Reviews 37, 97103.
Cousins, R. J., Smith, K. T., Failla, M. L. & Markowitz, L. A. (1978). Life Sciences 23, 18191826.
Davies, N. T. (1980). British Journal of Nutrition 43, 189203.
Elmes, M. E., Golden, M. I. & Love, A. H. G. (1976). Quarterly Journal of Medicine 45, 696697.
Flanagan, P. R., Haist, J. & Valberg, L. S. (1983). Journal of Nutrition 113, 962972.
Hall, A. C., Young, B. W. & Bremner, I. (1979). Journal of Inorganic Biochemistry 11, 5766.
Hurley, L. S., Duncan, J. R., Swan, M. V. & Eckhert, C. D. (1977). Proceedings of the National Academy of Sciences, USA 74, 35473549.
Jackson, M. J., Jones, D. A. & Edwards, R. H. T. (1981). British Journal of Nutrition 46, 1527.
Kowarski, S., Blair-Stanek, C. S. & Schachter, D. (1974). American Journal of Physiology 226, 401407.
Mehra, R. K. & Bremner, I. (1983). Biochemical Journal 214, 459465.
Menard, M. P., McCormick, C. C. & Cousins, R. J. (1981). Journal of Nutrition 111, 13531361.
Menard, M. P., Oestreicher, P. & Cousins, R. J. (1983). In Nutritional Bioavailability of zinc, American Chemical Society Symposium Series no. 210, pp. 233246 [Inglett, G.E., editor]. Washington DC: American Chemical Society.
Moynahan, E. J. (1974). Lancet ii, 399400.
Olafson, R. W. (1983). Journal of Nutrition 113 268275.
Richards, M. P. & Cousins, R. J. (1975). Biochemical and Biophysical Research Communications 64, 12151223.
Richards, M. P. & Cousins, R. J. (1976). Proceedings of the Society for Experimental Biology and Medicine 153, 5256.
Richards, M. P. & Cousins, R. J. (1977). Biochemical and Biophysical Research Communications 75, 286294.
Starcher, B. C., Glauber, J. C. & Madras, J. G. (1980). Journal of Nutrition 110, 13911397.
Sugawara, N. (1982). In Biological Roles of Metallothionein, pp. 155162 [Foulkes, E. C., editor]. New York: Elsevier/North-Holland.
Taguchi, T. & Nakamura, K. (1982). Journal of Toxicology an Environmental Health 9, 401409.
Webb, M. [editor] (1979). In The Chemistry, Biochemistry and Biology of Cadmium, pp. 195266. New York: Elsevier/North Holland.

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