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Ontogenic expression of the amino acid transporter b0,+AT in suckling Huanjiang piglets: effect of intra-uterine growth restriction

  • Wence Wang (a1) (a2), Francois Blachier (a3), Dezhi Fu (a2), Jie Pan (a4), Huansheng Yang (a2), Jieping Guo (a2), Wuying Chu (a5), Xiangfeng Kong (a2) (a6) and Yulong Yin (a2)...

Abstract

Intestinal amino acid (AA) transport is critical for the supply of AA to other tissues. Few studies regarding AA intestinal transport systems during the period from postnatal intense development of piglets until weaning are available. In the present study, we measured the intestinal expression of b0,+AT according to developmental stage using the suckling Huanjiang piglet model, and documented the effect of intra-uterine growth restriction (IUGR) on such expression using real-time PCR and Western blot analysis. Suckling piglets that recovered after IUGR and those with normal body weights (NBW) were used after birth or at 7, 14 and 21 d of age. Blood samples were used for the measurement of plasma AA concentrations, and the jejunum was collected for the measurement of b0,+AT expression. In NBW piglets, b0,+AT expression was markedly decreased from days 0 to 21 (P< 0·01) and remained at a low level during all the suckling periods. In IUGR piglets, there was a marked decrease in b0,+AT expression at birth, which remained lower, when compared with NBW piglets, during the suckling period. These results coincided with decreased plasma arginine concentration at birth and decreased lysine concentration in 21-d-old piglets (P< 0·05). It is concluded that the high expression of b0,+AT at birth decreases during the suckling period, and that IUGR is associated with decreased expression of this apical AA transporter. The possible causal relationship between decreased b0,+AT expression and lower body weight of IUGR piglets in the suckling period is discussed.

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Corresponding author

*Corresponding authors: Dr Y. Yin, email yinyulong@isa.ac.cn; X. Kong, email nnkxf@isa.ac.cn

References

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1Wu, G, Bazer, WF, Wallace, MJ, et al. (2006) Intrauterine growth retardation: implications for the animal sciences. J Anim Sci 84, 23162337.
2McMillen, IC & Robinson, JS (2005) Developmental origins of the metabolic syndrome: prediction, plasticity, and programming. Physiol Rev 85, 571633.
3Baserga, M, Bertolotto, C, Maclennan, NK, et al. (2004) Uteroplacental insufficiency decreases small intestine growth and alters apoptotic homeostasis in term intrauterine growth retarded rats. Early Hum Dev 79, 93105.
4Xu, RJ, Mellor, DJ, Birtles, MJ, et al. (1994) Impact of intrauterine growth retardation on the gastrointestinal tract and the pancreas in newborn pigs. J Pediatr Gastroenterol Nutr 18, 231240.
5D'Inca, R, Kloareg, M, Gras-Le Guen, C, et al. (2010) Intrauterine growth restriction modifies the developmental pattern of intestinal structure, transcriptomic profile, and bacterial colonization in neonatal pigs. J Nutr 140, 925931.
6Blachier, F, Boutry, C, Bos, C, et al. (2009) Metabolism and functions of l-glutamate in the epithelial cells of the small and large intestines. Am J Clin Nutr 90, 814S821S.
7Coloso, RM & Stipanuk, MH (1989) Metabolism of cyst(e)ine in rat enterocytes. J Nutr 119, 19141924.
8Yin, YL, Huang, RL, Li, TJ, et al. (2010) Amino acid metabolism in the portal-drained viscera of young pigs: effects of dietary supplementation with chitosan and pea hull. Amino Acids 39, 15811587.
9Shoveller, AK, Stoll, B, Ball, RO, et al. (2005) Nutritional and functional importance of intestinal sulfur amino acid metabolism. J Nutr 35, 16091612.
10Blachier, F, M'Rabet-Touil, H, Posho, L, et al. (1993) Intestinal arginine metabolism during development. Evidence for de novo synthesis of l-arginine in newborn pig enterocytes. Eur J Biochem 216, 109117.
11Chen, LX, Yin, YL, Jobgen, WS, et al. (2007) In vitro oxidation of essential amino acids by jejunal mucosal cells of growing pigs. Livest Sci 109, 1923.
12Chen, LX, Li, P, Wang, JJ, et al. (2009) Catabolism of nutritionally essential amino acids in developing porcine enterocytes. Amino Acids 37, 143152.
13Feliubadaló, L, Font, M, Purroy, J, et al. (1999) Non-type I cystinuria caused by mutations in SLC7A9, encoding a subunit (b0,+AT) of rBAT. Nat Genet 23, 5257.
14Munck, BG (1980) Lysine transport across the small intestine. Stimulating and inhibitory effects of neutral amino acids. J Membr Biol 53, 4553.
15Dello Strologo, L, Pras, E, Pontesilli, C, et al. (2002) Comparison between SLC3A1 and SLC7A9 cystinuria patients and carriers: a need for a new classification. J Am Soc Nephrol 13, 25472553.
16Dave, MH, Schulz, N, Zecevic, M, et al. (2004) Expression of heteromeric amino acid transporters along the murine intestine. Am J Physiol 258, 597610.
17Verrey, F, Closs, EI, Wagner, CA, et al. (2004) CATs and HATs: the SLC7 family of amino acid transporters. Pflüg Arch Eur J Physiol 447, 532542.
18Reig, N, Chillarón, J, Bartoccioni, P, et al. (2002) The light subunit of system b0,+ is fully functional in the absence of the heavy subunit. EMBO J 18, 49064914.
19Flynn, NE, Meininger, CJ, Haynes, TE, et al. (2002) The metabolic basis of arginine nutrition and pharmacotherapy. Biomed Pharmacother 56, 427438.
20Wu, G, Fang, YZ, Yang, S, et al. (2004) Glutathione metabolism and its implications for health. J Nutr 134, 489492.
21Zhao, DS (2008) Characteristic and feeding management of fragrant pigs. Livest Poult Husb 3, 1617.
22Yang, HS, Fu, DZ, Shao, H, et al. (2012) Impacts of birth weight on plasma, liver and skeletal muscle neutral amino acid profiles and intestinal amino acid transporters in suckling Huanjiang mini-piglets. PLoS One 7, e50921.
23Yang, HS, Li, FN, Yin, YL, et al. (2013) Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs. Mol Cell Endocrinol 365, 4451.
24He, QH, Ren, PP, Kong, XF, et al. (2012) Comparison of serum metabolite compositions between obese and lean growing pigs. J Nutr Biochem 23, 133139.
25Li, FN, Li, LL, Yang, HS, et al. (2011) Regulation of soy isoflavones on weight gain and fat percentage: evaluation in a Chinese Guangxi minipig model. Animal 5, 19031908.
26Yang, HS, Li, FN, Kong, XF, et al. (2012) Molecular cloning, tissue distribution and ontogenetic expression of Xiang pig chemerin and its involvement in regulating energy metabolism through Akt and ERK1/2 signaling pathways. Mol Biol Rep 39, 18871894.
27Zhang, M, Jing, RB & Cui, HB (1996) The investigation of growth development, reproductive characteristics and experimental animalization in fragrant pigs. Swine Prod 2, 3032.
28Yu, PC, Hua, XG, Xu, JX, et al. (2002) Measurements on growth and development and fattening performance of Xiang pigs. J Shanghai Jiaotong Univ (Agric Sci) 20, 228231.
29Zhang, Y, Liu, PQ & Luo, AQ (2008) Determination of fatty acids and nutritional evaluation in Scent pig muscle. Swine Prod 6, 3940.
30Reeds, PJ, Burrin, DG, Stoll, B, et al. (2000) Intestinal glutamate metabolism. J Nutr 130, 978S982S.
31Luo, G, Zhang, L, Liu, LS, et al. (2004) The applied research prospects of laboratory mini-pig in China. Lab Anim Sci Manage 2, 3738.
32He, QH, Ren, PP, Kong, XF, et al. (2011) Intrauterine growth restriction alters the metabonome of the serum and jejunum in piglets. Mol Bio Syst 7, 21472155.
33Liu, ZQ, Geng, MM, Shu, XG, et al. (2012) Dietary NCG supplementation enhances the expression of N-acetylglutamate synthase in intestine of weaning pig. J Food Agric Environ 10, 408412.
34Tang, ZR, Yin, LY, Nyachoti, CM, et al. (2005) Effect of dietary supplementation of chitosan and galacto-mannan-oligosaccharide on serum parameters and the insulin like growth factor-I mRNA expression in early-weaned piglets. Domes Anim Endocrinol 28, 430441.
35Yao, K, Yin, YL, Chu, WY, et al. (2008) Dietary arginine supplementation increases mTOR signaling activity in skeletal muscle of neonatal pigs. J Nutr 138, 867872.
36Yin, YL, Baidoo, SK, Schulze, H, et al. (2001) Effect of supplementing diets containing hulless barley varieties having different levels of non-starch polysaccharides with β-glucanase and xylanase on the physiological status of gastrointestinal tract and nutrient digestibility of weaned pigs. Livest Prod Sci 71, 97107.
37Kong, XF, Yin, FG, He, QH, et al. (2009) Acanthopanax senticosus extract as a dietary additive enhances the apparent ileal digestibility of amino acids in weaned piglets. Livest Sci 123, 261267.
38Wang, WC, Gu, WT, Tang, XF, et al. (2009) Molecular cloning, tissue distribution and ontogenetic expression of the amino acid transporter b0,+ cDNA in the small intestine of Tibetan suckling piglets. Compar Biochem Physiol Part B 154, 157164.
39Muller, PY, Janoviak, H, Miserez, AR, et al. (2002) Processing of gene expression data generated by quantitative real-time QPCR. Biotechniques 32, 13721374, 1376, 1378–1379.
40Wang, WC, Shi, CY, Zhang, JS, et al. (2009) Molecular cloning, distribution and ontogenetic expression of the oligopeptide transporter PepT1 mRNA in Tibetan suckling piglets. Amino Acids 37, 593601.
41Wolter, BF & Ellis, M (2001) The effect of weaning weight and rate of growth immediately after weaning on subsequent pig growth performance and carcass characteristics. Can J Anim Sci 81, 363369.
42Azain, MJ, Tomkins, T, Sowinski, JS, et al. (1996) Effect of supplemental pig milk replacer on litter performance: seasonal variation in response. J Anim Sci 74, 21952202.
43King, RH, Boyce, JM & Dunshea, FR (1998) Effect of supplemental nutrients on the growth performance of sucking pigs. Aust J Agric Res 49, 883887.
44Wolter, BF, Ellis, M, Corrigan, BP, et al. (2002) The effect of birth weight and feeding of supplemental milk replacer to piglets during lactation on preweaning and postweaning growth performance and carcass characteristics. J Anim Sci 80, 301308.
45Campbell, RG & Dunkin, AC (1982) The effect of birth weight on the estimated milk intake, growth and body composition of sow-reared piglets. Anim Prod 35, 193197.
46Xu, D, Phillips, JC & Schulten, K (1996) Protein response to external electric fields: relaxation, hysteresis, and echo. J Phys Chem 100, 1210812121.
47Blachier, F, Guihot-Joubrel, G, Vaugelade, P, et al. (1999) Portal hyperglutamatemia after dietary supplementation with monosodium glutamate in pigs. Digestion 60, 349357.
48Bertolo, RF, Pencharz, PB & Ball, RO (2000) Organ and plasma amino acid concentrations are profoundly different in piglets fed identical diets via gastric, central venous or portal venous routes. J Nutr 130, 12611266.
49Wang, J, Chen, L, Li, D, et al. (2008) Intrauterine growth restriction affects the proteomes of the small intestine, liver, and skeletal muscle in newborn pigs. J Nutr 138, 6066.
50Bröer, S (2008) Apical transporters for neutral amino acids: physiology and pathophysiology. Physiol (Bethesda) 23, 95103.
51Blomberg, LA, Schreier, LL, David Guthrie, H, et al. (2010) The effect of intrauterine growth retardation on the expression of developmental factors in porcine placenta subsequent to the initiation of placentation. Placenta 31, 549552.
52Thornbury, JC, Sibbons, PD, van Velzen, D, et al. (1993) Histological investigations into the relationship between low birth weight and spontaneous bowel damage in the neonatal piglet. Pediatr Pathol 13, 5969.
53Regnault, TRH, Friedman, JE, Wilkening, RB, et al. (2005) Fetoplacental transport and utilization of amino acids in IUGR – a review. Placenta 26, Suppl. A, S52S62.
54Wang, X, Wu, W, Lin, G, et al. (2010) Temporal proteomic analysis reveals continuous impairment of intestinal development in neonatal piglets with intrauterine growth restriction. J Proteome Res 9, 924935.
55Bisceglia, L, Fischetti, L & Bonis, PD (2010) Large rearrangements detected by MLPA, point mutations, and survey of the frequency of mutations within the SLC3A1 and SLC7A9 genes in a cohort of 172 cystinuric Italian patients. Mol Genet Metab 99, 4252.
56Ercolani, M, Sahota, A, Schuler, C, et al. (2010) Bladder outlet obstruction in male cystinuria mice. Int Urol Nephrol 42, 5763.
57Bröer, S. (2008) Amino acid transport across mammalian intestinal and renal epithelia. Physiol Rev 88, 249286.
58Sperandeo, MP, Annunziata, P, Bozzato, A, et al. (2007) Slc7a7 disruption causes fetal growth retardation by downregulating Igf1 in the mouse model of lysinuric protein intolerance. Am J Physiol Cell Physiol 293, 191198.

Keywords

Ontogenic expression of the amino acid transporter b0,+AT in suckling Huanjiang piglets: effect of intra-uterine growth restriction

  • Wence Wang (a1) (a2), Francois Blachier (a3), Dezhi Fu (a2), Jie Pan (a4), Huansheng Yang (a2), Jieping Guo (a2), Wuying Chu (a5), Xiangfeng Kong (a2) (a6) and Yulong Yin (a2)...

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