1. Delgado-Lista, J, Perez-Martinez, P, Lopez-Miranda, J, et al. (2012) Long chain omega-3 fatty acids and cardiovascular disease: a systematic review. Br J Nutr 107, S201–S213.
2. Calder, PC (2018) Very long-chain n-3 fatty acids and human health: fact, fiction and the future. Proc Nutr Soc 77, 52–72.
3. Food and Agriculture Organization (2016) State of World Fisheries and Aquaculture 2016. Rome: FAO of the United Nations.
4. Sprague, M, Dick, JR & Tocher, DR (2016) Impact of sustainable feeds on omega-3 long-chain fatty acid levels in farmed Atlantic salmon, 2006–2015. Sci Rep 6, 21892.
5. International Society for the Study of Fatty Acids and Lipids (2004) Report of the Sub-Committee on: Recommendations for Intake of Polyunsaturated Fatty Acids in Healthy Adults. Brighton: ISSFAL.
6. European Food Safety Authority (2010) Scientific opinion on dietary reference values for fat, including saturated fatty acids, polyunsaturated fatty acids, monounsaturated fatty acids, trans fatty acids, and cholesterol. EFSA panel on dietetic products, nutrition and allergies (NDA). EFSA J 8, 1461.
7. Salunkhe, DK, Adsule, RN, Chavan, JK, et al. (1992) World Oilseeds: Chemistry, Technology and Utilization. New York: VanNostrand Reinhold Company.
8. Ruiz-Lopez, N, Haslam, RP, Napier, JA, et al. (2014) Successful high-level accumulation of fish oil omega-3 long-chain polyunsaturated fatty acids in a transgenic oilseed crop. Plant J 77, 198–208.
9. Usher, S, Han, L, Haslam, RP, et al. (2017) Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa
. Sci Rep 7, 6570.
10. Betancor, MB, Sprague, M, Usher, S, et al. (2015) A nutritionally-enhanced oil from transgenic Camelina sativa effectively replaces fish oil as a source of eicosapentaenoic acid for fish. Sci Rep 5, 8104.
11. Betancor, MB, Sprague, M, Sayanova, O, et al. (2015) Evaluation of a high-EPA oil from transgenic Camelina sativa in feeds for Atlantic salmon (Salmo salar L.): effects on tissue fatty acid composition, histology and gene expression. Aquaculture 444, 1–12.
12. Betancor, MB, Sprague, M, Sayanova, O, et al. (2016) Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.). PLOS ONE 11, e0159934.
13. Betancor, MB, Li, K, Sprague, M, et al. (2017) An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry. PLOS ONE 12, e0175415.
14. Betancor, MB, Sprague, M, Montero, D, et al. (2016) Replacement of marine fish oil with de novo omega-3 oils from transgenic Camelina sativa in feeds for gilthead sea bream (Sparus aurata). Lipids 51, 1171–1191.
15. Montero, D & Izquierdo, M (2011) Welfare and health of fish fed vegetable oils as alternative lipid sources to fish oil. In Fish Oil Replacement and Alternative Lipid Sources in Aquaculture Feeds, pp. 439–485 [GM Turchini, WK Ng and DR Tocher, editors]. Boca Raton, FL: CRC Press.
16. Tort, L (2011) Stress and immune modulation in fish. Dev Comp Immunol 35, 1366–1375.
17. Austreng, E (1978) Digestibility determination in fish using chromic oxide marking and analysis of contents from different segments of the gastrointestinal tract. Aquaculture 13, 265–272.
18. Association of Official Analytical Chemists (2000) Official Methods of Analysis. Washington, DC: AOAC.
19. Folch, J, Lees, N & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226, 497–509.
20. Christie, WW (2003) Lipid Analysis, 3rd ed. Bridgwater: Oily Press.
21. Tocher, DR & Harvie, DG (1988) Fatty acid compositions of the major phosphoglycerides from fish neural tissues; (n-3) and (n-6) polyunsaturated fatty acids in rainbow trout (Salmo gairdneri) and cod (Gadus morhua) brains and retinas. Fish Physiol Biochem 5, 229–239.
22. Choi, HS, Kim, JW, Cha, YN, et al. (2006) A quantitative nitroblue tetrazolium assay for determining intracellular superoxide anion production in phagocytic cells. J Immunoassay Immunochem 27, 31–44.
23. Secombes, CJ (1990) Isolation of salmonid macrophages and analysis of their killing activity. In Techniques in Fish Immunology, pp. 137–154 [JS Stolen, TC Fletcher, DP Anderson, et al., editors]. New Haven, NJ: SOS Publication.
24. Pulsford, AL, Crampe, M, Lagnston, A, et al. (1995) Modulatory effects of disease, stress, copper, TBT and vitamin E on the immune system of flatfish. Fish Shellfish Immunol 5, 631–643.
25. Russo, R, Shoemaker, CA, Panangala, VS, et al. (2009)
In vitro and in vivo interaction of macrophages from vaccinated and non-vaccinates channel catfish (Ictalurus punctatus) to Edwarsiella ictaluri
. Fish Shellfish Immunol 26, 543–552.
26. Jensch-Junior, BE, Presinotti, LN, Borges, JCS, et al. (2006) Characterization of macrophage phagocytosis of the tropical fish Prochilodus scrofa (Steindachner, 1881). Aquaculture 251, 509–515.
27. Morais, S, Edvardsen, RB, Tocher, DR, et al. (2012) Transcriptomic analyses if intestinal gene expression of juvenile Atlantic cod (Gadus morhua) fed diets with Camelina oil as replacement for fish oil. Comp Biochem Physiol 161B, 283–293.
28. Tacchi, L, Secombes, CJ, Bickerdike, R, et al. (2012) Transcriptomic and physiological responses to fishmeal substitution with plant proteins in formulated feed in farmed Atlantic salmon (Salmo salar). BMC Genomics 13, 363.
29. Martinez-Rubio, L, Evensen, Ø, Krasnov, A, et al. (2014) Effects of functional feeds on the lipid composition, transcriptomic responses and pathology in heart of Atlantic salmon (Salmo salar L.) before and after experimental challenge with Piscine Myocarditis Virus (PMCV). BMG Genomics
30. Bicskei, B, Bron, JE, Glover, KA, et al. (2014) A comparison of gene transcription profiles of domesticated and wild Atlantic salmon (Salmo salar L.) at early life stages, reared under controlled conditions. BMC Genomics 15, 884.
31. Betancor, MB, Howarth, FJE, Glencross, BD, et al. (2014) Influence of dietary docosahexaenoic acid in combination with other long-chain polyunsaturated fatty acids on expression of biosynthesis genes and phospholipid fatty acid compositions in tissues of post-smolt Atlantic salmon (Salmo salar). Comp Biochem Physiol B 172–173, 74–89.
32. Rozen, S & Skaletsky, H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132, 365–386.
33. Kuleshov, MV, Jones, MR, Rouillard, AD, et al. (2016) Enrichr: a comprehensive gene set enrichment analysis web server 2016 update. Nucleic Acids Res 8, W90–W97.
34. National Research Council (2011) Nutrient Requirements of Fish and Shrimp. Washington, DC: The National Academies Press.
35. Dentin, R, Benhamed, F, Pégorier, JP, et al. (2005) Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation. J Clin Invest 115, 2843–2854.
36. Morais, S, Pratoomyot, J, Torstensen, BE, et al. (2011) Diet x genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon (Salmo salar) in response to replacement of dietary fish oil with vegetable oil. Br J Nutr 106, 1457–1469.
37. Menoyo, D, Lopez-Bote, CJ, Diez, A, et al. (2007) Impact of n-3 fatty acid chain length and n-3/n-6 ratio in Atlantic salmon (Salmo salar) diets. Aquaculture 267, 248–259.
38. Martins, D, Valente, LMP & Lall, SP (2009) Apparent digestibility of lipid and fatty acids in fish oil poultry fat and vegetable oil diets by Atlantic halibut, Hippoglossus hippoglossus L. Aquaculture 294, 132–137.
39. Sigurgisladottir, S, Lall, SP, Parrish, CC, et al. (1992) Cholestane as a digestibility marker in the absorption of polyunsaturated fatty acid ethyl esters in Atlantic salmon. Lipids 27, 418–424.
40. Caballero, MJ, Obach, A, Rosenlund, G, et al. (2002) Impact of different dietary lipid sources on growth, lipid digestibility, tissue fatty acid composition and histology of rainbow trout, Oncorhynchus mykiss
. Aquaculture 214, 253–271.
41. Johnsen, RI, Grahl-Nielsen, O & Roem, A (2000) Relative absorption of fatty acids by Atlantic salmon Salmo salar from different diets, as evaluated by multivariate statistics. Aquacult Nutr 6, 255–261.
42. Ng, WK, Sigholt, T & Bell, JG (2004) The influence of environmental temperature on the apparent nutrient and fatty acid digestibility in Atlantic salmon (Salmo salar L.) fed finishing diets containing different blends of fish oil, rapeseed oil and palm oil. Aquacult Res 35, 1228–1237.
43. Morais, S, Monroig, O, Zhen, X, et al. (2009) Highly unsaturated fatty acid synthesis in Atlantic salmon: characterization of ELOVL5- and ELOVL2-like elongases. Marine Biotechnol 11, 627–639.
44. Monroig, O, Zheng, X, Morais, S, et al. (2010) Mutiple genes for functional Δ6 fatty acyl desaturases (Fad) in Atlantic salmon (Salmo salar L.): gene and cDNA characterization, functional expression, tissue distribution and nutritional regulation. Biochim Biophys Acta Mol Cell Biol Lipids 1801, 1072–1081.
45. Vagner, M & Santigosa, E (2011) Characterization and modulation of gene expression and enzymatic activity of delta-6-desaturase in teleost: a review. Aquaculture 315, 131–143.
46. Bell, JG, Tocher, DR, Henderson, RJ, et al. (2003) Altered fatty acid compositions in Atlantic salmon (Salmo salar) fed diets containing linseed and rapeseed oils can be partially restored by a subsequent fish oil finishing diet. J Nutr 33, 2793–2801.
47. Bransden, MP, Carter, CG & Nichols, PD (2003) Replacement of fish oil with sunflower oil in feeds for Atlantic salmon (Salmo salar L.): effect on growth performance, tissue fatty acid composition and disease resistance. Comp Biochem Physiol B 135, 611–625.
48. Gjøen, T, Obach, A, Røsjø, C, et al. (2004) Effect of dietary lipids on macrophage function, stress susceptibility and disease resistance in Atlantic salmon (Salmo salar). Fish Physiol Biochem 30, 149–161.
49. Tort, L, Balasch, JC & Mackenzie, S (2003) Fish immune system. A crossroads between innate and adaptive responses. Immunologia 22, 277–286.
50. Gjøen, T, Kleveland, EJ, Moya-Falcón, C, et al. (2007) Effects of dietary thia fatty acids on lipid composition, morphology and macrophage function of Atlantic salmon (Salmo salar L.) kidney. Comp Biochem Physiol 148B, 103–111.
51. Waagbø, R (1994) The impact of nutritional factors on the immune system in Atlantic salmon, Salmo salar L: a review. Aquacult Res 25, 175–197.
52. Lall, SP (2000) Nutrition and health of fish. In Avances en Nutricion Acuicola V. Proceedings of the V International Symposium on Fish Nutrition, 19–22 November 2000 pp. 13–23 [LE Cruz-Suarez, D Ricque-Marie, M Tapia-Salazar, et al., editors]. Merida, Yucatan.
53. Martínez-Rubio, L, Wadsworth, S, Vecino, JLG, et al. (2013) Effect of dietary digestible energy content on expression of genes of lipid metabolism and LC-PUFA biosynthesis in liver of Atlantic salmon (Salmo salar L). Aquaculture 384-387, 94–103.
54. Bell, JG, Ashton, I, Secombes, CJ, et al. (1996) Dietary lipid affects phospholipid fatty acid compositions, eicosanoid production and immune function in Atlantic salmon (Salmo salar). Prostaglandins Leukot Essent Fatty Acids 54, 73–182.
55. Seierstad, S, Haugland, Ø, Waagbø, R, et al. (2009) Pro-inflammatory cytokine expression and respiratory burst activity following replacement of fish oil with rapeseed oil in the feed for Atlantic salmon (Salmo salar L.). Aquaculture 289, 212–218.
56. Dantagnan, P, Gonzalez, K, Hevia, M, et al. (2017) Effect of the arachidonic acid/vitamin E interaction on the immune response of juvenile Atlantic salmon (Salmo salar) challenged against Piscirickettsia salmonis
. Aquac Nutr 23, 710–720.
57. Montero, D, Grasso, V, Izquierdo, MS, et al. (2008) Total substitution of fish oil by vegetable oils in gilthead sea bream (Sparus aurata) diets: effects on hepatic Mx expression and some immune parameters. Fish Shellfish Immunol 24, 147–155.
58. Ganga, R, Bell, JG, Montero, D, et al. (2005) Effect of feeding gilthead seabream (Sparus aurata) with vegetable lipid sources on two potential immunomodulatory products: prostanoids and leptins. Comp Biochem Physiol 142, 410–418.
59. Yaqoob, P (2004) Fatty acids and the immune system: from basic science to clinical applications. Proc Nutr Soc 63, 89–104.
60. Montero, D, Mathlouthi, F, Tort, L, et al. (2010) Replacement of dietary fish oil by vegetable oil affects humoral immunity and expression of pro-inflammatory cytokines genes in gilthead sea bream Sparus aurata
. Fish Shellfish Immunol 29, 1073–1081.
61. Seppola, M, Mikkelsen, H, Johansen, A, et al. (2015) Ultrapure LPS induces inflammatory and antibacterial responses in vitro by exogenous sera in Atlantic cod and Atlantic salmon. Fish Shellfish Immunol 44, 66–78.
62. Mashek, DG, Li, LO & Coleman, RA (2006) Rat long-chain acyl-coA synthetase mRNA, protein, and activity vary in tissue distribution and in response to diet. J Lipid Res 47, 2004–2010.
63. Tian, JJ, Lei, CX, Ji, H, et al. (2017) Role of cyclooxygenase-mediated metabolites in lipid metabolism and expression of some immune-related genes in juvenile grass carp (Ctenopharyngodon idellus) fed arachidonic acid. Fish Physiol Biochem 43, 703–717.
64. Torstensen, BE, Nanton, DA, Olsvik, PA, et al. (2009) Gene expression of fatty acid-binding proteins, fatty acid transport proteins (cd36 and FATP) and β-oxidation-related genes in Atlantic salmon (Salmo salar L.) fed fish oil or vegetable oil. Aquacult Nutr 15, 440–451.
65. Dong, X, Tan, P, Cai, Z, et al. (2017) Regulation of FADS2 transcription by SREBP-1 and PPAR-α influences LC-PUFA biosynthesis in fish. Sci Rep 7, 40024.
66. Morais, S, Pratoomyot, J, Taggart, JB, et al. (2011) Genotype-specific responses in Atlantic salmon (Salmo salar) subject to dietary fish oil replacement by vegetable oil: a liver transcriptomic analysis. BMC Genomics 12, 255.