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Nucleic acid metabolism in the ruminant

Determination of nucleic acids in digesta

Published online by Cambridge University Press:  09 March 2007

A. B. Mcallan  and
R. H. Smith
A. B. Mcallan
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
R. H. Smith
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
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Abstract

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1. Procedures, based on those of Schmidt & Thannhauser (1945) and Schneider (1945), for the extraction and estimation of nucleic acids in bovine digesta were examined in detail.

2. Final methods which were suitable for routine determination of RNA and DNA were essentially as follows. Digesta samples were extracted in the cold, first with a solution of trichloroacetic acid in ethanol, then with aqueous trichloroacetic acid solution and finally with lipid solvents. The dried residue was hydrolysed with alkali, purified by passage through a Dowex resin, and the RNA, in the form of mononucleotides, determined by U.V. absorption. DNA was determined separately in hot perchloric acid extracts of the original dried residue by colorimetric estimation of the deoxyribose content.

Type
Research Article
Information
British Journal of Nutrition , Volume 23 , Issue 3 , August 1969 , pp. 671 - 682
Copyright
Copyright © The Nutrition Society 1969

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