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n−3 PUFA fail to affect in vivo, antigen-driven CD8+T-cell proliferation in the spleen of naïve mice

Published online by Cambridge University Press:  08 March 2007

Robert Irons  and
Kevin L. Fritsche
Robert Irons
Affiliation:
Nutritional Sciences Program, University of Missouri, Columbia, MO 65211, USA
Kevin L. Fritsche*
Affiliation:
Nutritional Sciences Program, University of Missouri, Columbia, MO 65211, USA Division of Animal Sciences, Department of Microbiology and Molecular Immunology, University of Missouri, Columbia, MO 65211, USA
*
*Corresponding author: Dr Kevin L. Fritsche, Division of Animal Sciences, 920 E. Campus Dr., 110 ASRC, Columbia, MO 65211, USA, fax 573 882 6827, email FritscheK@missouri.edu
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Abstract

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One of the most frequently reported immunomodulatory actions of n−3 PUFA is their ability to diminish in vivo lymphocyte proliferation. The purpose of this study was to determine if n−3 PUFA intake affects the kinetics or magnitude of the antigen-driven expansion of CD8+T-lymphocytes in vivo. In this study we utilized a well-characterized model of T-cell immunity (i.e. infection with the intracellular bacterium, Listeria monocytogenes). Weanling BALB/c mice were fed one of two experimental diets that differed solely in fat source. Our control diet contained lard (180g/kg) and was devoid of long-chain n−3 PUFA. The experimental diet contained 150g/kg menhaden fish oil and 30g/kg corn oil, thus providing approximately 8% of energy from long-chain n−3 PUFA. After 4 weeks, mice were infected intravenously with 106 colony-forming units of actA-deficient L. monocytogenes. Clonal expansion of antigen-specific CD8+T-cells in the spleen was measured at 5, 7, 9 and 14d post-challenge using a class I MHC tetramer loaded with the immunodominant peptide from this pathogen (i.e. Kd:LLO91–99). We report that feeding mice a diet rich in n−3 fatty acids did not significantly impact either the kinetics or magnitude of in vivo, antigen-driven expansion of CD8+T-cells. Furthermore, contraction of this T-cell population was not affected by n−3 PUFA treatment. To our knowledge this is the first time MHC tetramers have been used to investigate the influence of n−3 PUFA on in vivo CD8+T-cell proliferation.

Keywords

n−3 PUFACD8+T-cellProliferationMHC tetramerListeriaMice
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 4 , April 2006 , pp. 838 - 844
Copyright
Copyright © The Nutrition Society 2006

References

Anderson, MJ & Fritsche, KLDietary polyunsaturated fatty acids modulate in vivo, antigen-driven CD4+T-cell proliferation in mice. J Nutr (2004) 134, 1978–1983.CrossRefGoogle ScholarPubMed
Arrington, JL, Chapkin, RS, Switzer, KC, Morris, JS & McMurray, DNDietary n-3 polyunsaturated fatty acids modulate purified murine T-cell subset activation. Clin Exp Immunol (2001) 125, 499–507.CrossRefGoogle ScholarPubMed
Badovinac, VPPorter, BB & Harty, JTProgrammed contraction of CD8(+) T cells after infection. Nat Immunol (2002) 3, 619–626.CrossRefGoogle ScholarPubMed
Bishop, DK & Hinrichs, DJAdoptive transfer of immunity to Listeria monocytogenes. The influence of in vitro stimulation on lymphocyte subset requirements. J Immunol (1987) 139, 2005–2009.CrossRefGoogle ScholarPubMed
Busch, DH, Kerksiek, KM & Pamer, EGDiffering roles of inflammation and antigen in T cell proliferation and memory generation. J Immunol (2000) 164, 4063–4070.CrossRefGoogle Scholar
Busch, DH, Pilip, IM, Vijh, S & Pamer, EGCoordinate regulation of complex T cell populations responding to bacterial infection. Immunity (1998) 8, 353–362.CrossRefGoogle ScholarPubMed
Byleveld, M, Pang, G, Clancy, R & Roberts, DFish oil feeding enhances lymphocyte proliferation but impairs virus-specific T lymphocyte cytotoxicity in mice following challenge with influenza virus. Clin Exp Immunol (2000) 119, 287–292.CrossRefGoogle ScholarPubMed
Calder, PC, Yaqoob, P, Thies, F, Wallace, FA & Miles, EAFatty acids and lymphocyte functions. Br J Nutr (2002) 87, Suppl 1, S31–S48.CrossRefGoogle ScholarPubMed
Czuprynski, CJ & Haak-Frendscho, MNon-specific resistance mechanisms to listeriosis: implications for experimental and naturally occurring infection. Immunol Rev (1997) 158, 47–56.CrossRefGoogle ScholarPubMed
de Pablo, MA, Puertollano, MA, Galvez, A,Galvez, A,Ortega, E, Gaforio, JJ & Alvarez de Cienfuegos, GDetermination of natural resistance of mice fed dietary lipids to experimental infection induced by Listeria monocytogenes. FEMS Immunol Med Microbiol (2000) 27, 127–133.CrossRefGoogle ScholarPubMed
Dubey, C, Croft, M & Swain, SLNaive and effector CD4 T cells differ in their requirements for T cell receptor versus costimulatory signals. J Immunol (1996) 157, 3280–3289.CrossRefGoogle Scholar
Engwerda, CR & Kaye, PMOrgan-specific immune responses associated with infectious disease. Immunol Today (2000) 21, 73–78.CrossRefGoogle ScholarPubMed
Farber, DL & Ahmadzadeh, MDissecting the complexity of the memory T cell response. Immunol Res (2002) 25, 247–259.CrossRefGoogle ScholarPubMed
Fowler, KH, Chapkin, RS & McMurrray, DNEffects of purified dietary n-3 ethyl esters on murine T lymphocyte function. J Immunol (1993) 151, s186–S197.CrossRefGoogle Scholar
Fritsche, KL, Shahbazian, LM, Feng, C & Berg, JNDietary fish oil reduces survival and impairs bacterial clearance in C3H/Hen mice challenged with Listeria monocytogenes. Clin Sci (1997) 92, 95–101.CrossRefGoogle ScholarPubMed
Harbige, LSDietary n-6 and n-3 fatty acids in immunity and autoimmune disease. Proc Nutr Soc (1998) 57, 555–562.CrossRefGoogle ScholarPubMed
Harbige, LS & Fisher, BADietary fatty acid modulation of mucosally-induced tolerogenic immune responses. Proc Nutr Soc (2001) 60, 449–456.CrossRefGoogle ScholarPubMed
Huang, SC, Misfeldt, ML & Fritsche, KLDietary fat influences Ia antiger expression and immune cell populations in the murine peritoneum and spleen. J Nutr (1992) 122, 1219–1231.CrossRefGoogle Scholar
Irons, R, Anderson, MJ, Zhang, M & Fritsche, KLDietary fish oil impairs primary host resistance against Listeria monocytogenes more than the immunological memory response. J Nutr (2003) 133, 1163–1169.CrossRefGoogle Scholar
Jeffery, NM, Sanderson, P, Sherrington, P, Newsholme, EA, & Calder, PCThe ratio of n-6 to n-3 polyunsaturated fatty acids in the rat diet alters serum lipid levels and lymphocyte functions. Lipids (1996) 31, 737–745.CrossRefGoogle ScholarPubMed
Jenkins, MK, Khoruts, A, Ingulli, E, Mueller, DL, McSorley, SJ, Reinhardt, RL, Itano, A & Pape, KAIn vivo activation of antigen-specific CD4 T cells. Annu Rev Immunol (2001) 19, 23–45.CrossRefGoogle ScholarPubMed
Kaufmann, SHListeria monocytogenes specific T-cell lines and clones. Infection (1988) 16, Suppl. 2, S128–S136.CrossRefGoogle ScholarPubMed
Kelley, DS, Nelson, GJ, Branch, LB, Taylor, PC, Rivera, YM & Schmidt, PCSalmon diet and human immune status. Eur J Clin Nutr (1992) 46, 397–404.Google ScholarPubMed
Kew, s, Banerjee, T, Minihane, AM, Finnegan, YE, Muggli, R, Albers, R, Williams, CM & Calder, PCLack of effect of foods enriched with plant- or marine-derived n-3 fatty acids on human immune function. Am J Clin Nutr (2003) 77, 1287 – 1295.CrossRefGoogle ScholarPubMed
Kobayashi, K, Kaneda, K & Kasama, TImmunopathogenesis of delayed-type hypersensitivity. Microsc Res Tech (2001) 53, 241 – 245.CrossRefGoogle ScholarPubMed
Lanzavecchia, A & Sallusto, FUnderstanding the generation and function of memory T cell subsets. Curr Opin Immunol (2005) 17, 326 – 332.CrossRefGoogle ScholarPubMed
Lyons, ABAnalysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J Immunol Methods (2000) 243, 147 – 154.CrossRefGoogle ScholarPubMed
McGuire, SO, Alexander, DW & Fritsche, KLFish oil source differentially affects rat immune cell a-tocopherol concentration. J Nutr (1997) 127, 1388 – 1394.CrossRefGoogle Scholar
McKay, DB & Lu, CYListeriolysin as a virulence factor in Listeria monocytogenes infection of neonatal mice and murine decidual tissue. Infect Immun (1991) 59, 4286 – 4290.CrossRefGoogle ScholarPubMed
Merica, R, Khoruts, A, Pape, KA, Reinhardt, RL & Jenkins, MKAntigen-experienced CD4 T cells display a reduced capacity for clonal expansion in vivo that is imposed by factors present in the immune host. J Immunol (2000) 164, 4551 – 4557.CrossRefGoogle ScholarPubMed
Meydani, SN, Lichtenstein, AH, Cornwall, S, Meydani, M, Goldin, BR, Rasmussen, H, Dinarello, CA & Schaefer, EJImmunologic effects of national cholesterol education panel step-2 diets with and without fish-derived n-3 fatty acid enrichment. J Clin Invest (1993) 92, 105 – 113.CrossRefGoogle ScholarPubMed
Pamer, EG, Harty, JT & Bevan, MJPrecise prediction of a dominant class I MHC-restricted epitope of Listeria monocytogenes. Nature (1991) 353, 852 – 855.CrossRefGoogle Scholar
Pompos, LJ & Fritsche, KLAntigen-driven murine CD4+T lymphocyte proliferation and interleukin-2 production are diminished by dietary (n-3) polyunsaturated fatty acids. J Nutr (2002) 132, 3293 – 3300.CrossRefGoogle ScholarPubMed
Pope, C, Kim, SK, Marzo, A, Masopust, D, Williams, K, Jiang, J, Shen, H & Lefrancois, LOrgan-specific regulation of theCD8Tcell response to Listeria monocytogenes infection. J Immunol (2001) 166, 3402 – 3406.CrossRefGoogle ScholarPubMed
Puertollano, MA, de Pablo, MA & Alvarez de Cienfuegos, GImmunomodulatory effects of dietary lipids alter host natural resistance of mice to Listeria monocytogenes infection. FEMS Immunol Med Microbiol (2001) 32, 47 – 52.CrossRefGoogle ScholarPubMed
Reeves, PG, Nielsen, FH & Fahey, GCAIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr (1993) 123, 1939 – 1951.CrossRefGoogle Scholar
Sanderson, P & Calder, PCDietary fish oil diminishes lymphocyte adhesion to macrophage and endothelial cell monolayers. Immunology (1998) 94, 79 – 87.CrossRefGoogle ScholarPubMed
Switzer, KC, Fan, YY, Wang, N, McMurray, DN & Chapkin, RSDietary n-3 polyunsaturated fatty acids promote activation-induced cell death in Th1-polarized murine CD4+T-cells. J Lipid Res (2004) 45, 1482 – 1492.CrossRefGoogle ScholarPubMed
Switzer, KC, McMurray, DN, Morris, JS & Chapkin, RS(n-3) Polyunsaturated fatty acids promote activation-induced cell death in murine T lymphocytes. J Nutr (2003) 133, 496 – 503.CrossRefGoogle ScholarPubMed
Unanue, ERStudies in listeriosis show the strong symbiosis between the innate cellular system and the T-cell response. Immunol Rev (1997) 158, 11 – 25.CrossRefGoogle ScholarPubMed
Wallace, FA, Miles, EA & Calder, PCComparison of the effects of linseed oil and different doses of fish oil on mononuclear cell function in healthy human subjects. Br J Nutr (2003) 89, 679 – 689.CrossRefGoogle ScholarPubMed
Wijendran, V & Hayes, KCDietary n-6 and n-3 fatty acid balance and cardiovascular health. Annu Rev Nutr (2004) 24, 597 – 615.CrossRefGoogle ScholarPubMed
Yaqoob, P, Newsholme, EA & Calder, PCThe effect of dietary lipid manipulation on rat lymphocyte subsets and proliferation. Immunology (1994) 82, 603 – 610.Google ScholarPubMed
Zinkernagel, RMUncertainties - discrepancies in immunology. Immunol Rev (2002) 185, 103 – 125.CrossRefGoogle ScholarPubMed