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Mammary sensitivity to protein restriction and re-alimentation

Published online by Cambridge University Press:  09 March 2007

M. G. Goodwill ,
N. S. Jessop  and
J. D Oldham
M. G. Goodwill
Affiliation:
Institute of Ecology and Resource Management, The University of Edinburgh, West Mains Road, Edinburgh EH9 3JG
N. S. Jessop
Affiliation:
Institute of Ecology and Resource Management, The University of Edinburgh, West Mains Road, Edinburgh EH9 3JG
J. D Oldham
Affiliation:
Genetics and Behavioural Sciences Department, Scottish Agricultural College, West Mains Road, Edinburgh EH9 3JG
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Abstract

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The present study tested the influence of protein undernutrition and re-alimentation on mammary gland size and secretory cell activity in lactating rats. During gestation, female Sprague-Dawley rats were offered a high-protein diet (215 g crude protein (N x 625; CP)/kg DM; H); litters were standardized to twelve pups at parturition. During lactation, two diets were offered ad libitum, diet H and a low- protein diet (90 g CP/kg DM; L). Lactational dietary treatments were the supply ad libitum of either diet H (HHH) or diet L (LLL) for the first 12 d of lactation, or diet L transferring to diet H on either day 6 (LHH) or 9 (LLH) of lactation. On days 1, 6, 9 and 12 of lactation, rats from each group (n > 6) were used to estimate mammary dry mass, fat, protein, DNA and RNA; the activities of lactose synthetase (EC 2.4.1.22) enzyme and Na+, K+-ATPase (EC 3.6.1.37) were also measured. Rats offered a diet considered protein sufficient (H) from day 1 of lactation showed a decrease in mammary dry mass and fat but an increase in DNA, RNA and protein on day 6, after which there was no further change, except for mammary protein which continued to increase. However, rats offered diet L showed a steady loss in mammary mass and fat throughout the 12 d lactation period and no change in mammary DNA, RNA or protein. Rats previously protein restricted for either the first 6 or 9 d of lactation had their mammary dry mass and mammary fat loss halted and showed a rapid increase in mammary DNA, RNA and protein on re-alimentation. Lactose production in group HHH, as measured by lactose synthetase activity, was similar on days 1 and 6 of lactation, after which a significant increase was seen. Protein- restricted rats showed no change in lactose synthetase activity during the 12 d experimental period. Changing from diet L to diet H led to a significant increase in lactose synthetase activity to levels comparable with those offered diet H from day 1. These results show that rats offered a protein-restricted diet during lactation suffer mammary underdevelopment, but this may be rapidly reversed by re-alimentation with a high-protein diet

Keywords

LactationMammary glandDietary protein
Type
General Nutrition
Information
British Journal of Nutrition , Volume 76 , Issue 3 , September 1996 , pp. 423 - 434
Copyright
Copyright © The Nutrition Society 1996

References

REFERENCES

Anderson, R. R. (1975). Mammary gland growth in sheep. Journal of Animal Science 41, 118–123.CrossRefGoogle ScholarPubMed
Bohmer, F. D., Lehman, W., Schmidt, H. E., Langen, P., & Grosse, R. (1984). Purification of a growth inhibitor for Ehrlich ascites mammary carcinoma cells from bovine mammary gland. Experimental Cellular Research 150, 466–476.CrossRefGoogle ScholarPubMed
Brookreson, A. D. & Turner, C. W. (1959). Normal growth of mammary glands in pregnant and lactating mice. Proceedings of the Society for Experimental Biology 102, 744–745.CrossRefGoogle ScholarPubMed
Broster, W. H., Broster, V. J. & Smith, T. (1969). Experiments on the nutrition of the dairy heifer VIII. Effect on milk production of level of feeding at two stages of lactation. Journal of Agricultural Science 72, 229–245.CrossRefGoogle Scholar
Burgoyne, R. D. & Wilde, C. J. (1994). Control of secretory function in mammary epithelial cells. Cellular Signalling 6, 607–616.CrossRefGoogle ScholarPubMed
Carrick, D. T. & Kuhn, N. J. (1978). Diurnal variation and response to food withdrawal of lactose synthesis in lactating rats. Biochemical Journal 174, 319–325.CrossRefGoogle ScholarPubMed
Crnic, L. S. & Chase, H. P. (1978). Models of infantile undernutrition in rats: effects on milk composition. Journal of Nutrition 108, 1755–1760.CrossRefGoogle Scholar
Griffith, D. R. & Turner, C. W. (1957). Deoxyribonucleic acid (DNA) content of the mammary gland during pregnancy and lactation. Proceedings of the Society for Experimental Biology and Medicine 95, 347–348.CrossRefGoogle ScholarPubMed
Griffith, D. R. & Turner, C. W. (1961). Normal growth of the rat mammary glands during pregnancy and early lactation. Proceedings of the Society for Experimental Biology 106, 448–450.CrossRefGoogle ScholarPubMed
Knight, C. H. & Peaker, M. (1982 a). Effects of fasting during mid pregnancy or early lactation on mammary development and milk yield in mice. Journal of Dairy Research 49, 567–575.CrossRefGoogle ScholarPubMed
Knight, C. H. & Peaker, M. (1982 b). Mammary cell proliferation in mice during pregnancy and lactation in relation to milk yield. Quarterly Journal of Experimental Physiology 67, 165–177.CrossRefGoogle ScholarPubMed
Knight, C. H. & Peaker, M. (1982 c). Development of the mammary gland. Journal of Reproduction and Fertility 65, 521–536.CrossRefGoogle ScholarPubMed
Knight, C. H. & Peaker, M. (1984). Mammary development and regression during lactation in goats in relation to milk secretion. Quarterly Journal of Experimental Physiology 69, 331–338.CrossRefGoogle ScholarPubMed
Kurtz, A., Vogel, F., Funa, K., Heldin, C. H. & Grosse, R. (1990). Developmental regulation of mammary-derived growth inhibitor expression of bovine mammary tissue. Journal of Cell Biology 110, 1779–1789.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Lu, M. H. & Anderson, R. R., (1973). Growth of the mammary gland during pregnancy and lactation in the rabbit. Biology of Reproduction 9, 538–543.CrossRefGoogle ScholarPubMed
Mansaray, Y. K. C. & Grimble, R. (1983). Lactose synthetase (EC 2.4.1.22) activity in rats receiving diets of varying protein adequacy. Proceedings of the Nutrition Society 42, 140A.Google Scholar
Milligan, L. P. & Summers, M. (1986). The biological basis of maintenance and its relevance to assessing responses to nutrients. Proceedings of the Nutrition Society 45, 185–193.CrossRefGoogle ScholarPubMed
Mirsky, A. E. & Ris, H. (1949). Variable and constant components of chromosomes. Nature 163, 666–667.CrossRefGoogle ScholarPubMed
National Research Council (1978). Nutrient Requirements of Laboratory Animals, 3rd ed. Washington, DC:National Academy of Sciences.Google Scholar
Paape, M. J., Sinha, Y. N. (1971). Nucleic acid and collagen content of mammary glands between 30 and 80 days of age in normal and ovariectomised rats and during pregnancy. Journal of Dairy Science 54, 1068–1074.CrossRefGoogle Scholar
Palmiter, D. (1969). Properties of lactose synthetase from mouse mammary gland: role of a proposed third component. Biochimica et Biophysica Acta 178, 35–46.CrossRefGoogle ScholarPubMed
Pine, A. P., Jessop, N. S., Allan, G. F. & Oldham, J. D. (1994 a). Maternal protein reserves and their influence on lactational performance in rats 2. Effects of dietary protein restriction during gestation and lactation on tissue protein metabolism and Na+, K+-ATPase (EC 3.6.1.3) activity. British Journal of Nutrition 72, 181–197.CrossRefGoogle Scholar
Pine, A. P., Jessop, N. S., Allan, G. F. & Oldham, J. D. (1994 b). Maternal protein reserves and their influence on lactational performance in rats 3. The effects of dietary protein restriction and stage of lactation on milk composition. British Journal of Nutrition 72, 815–830.CrossRefGoogle ScholarPubMed
Pine, A. P., Jessop, N. S., Allan, G. F. & Oldham, J. D. (1994 c). Maternal protein reserves and their influence on lactational performance in rats 4. Tissue protein synthesis and turnover associated with mobilization of maternal protein. British Journal of Nutrition 72, 831–844.CrossRefGoogle ScholarPubMed
Pine, A. P., Jessop, N. S. & Oldham, J. D. (1994 d). Maternal protein reserves and their influence on lactational performance in rats. British Journal of Nutrition 71, 13–27.CrossRefGoogle ScholarPubMed
Pyska, H. & Styczynski, H. (1979 a). Effect of various protein levels in the diet on mammary gland growth in rats. Journal of Dairy Research 46, 551–554.CrossRefGoogle ScholarPubMed
Pyska, H. & Styczynski, H. (1979 b). Effect of dietary protein level during pregnancy and 2 weeks of lactation on mammary gland growth in rats. Journal of Dairy Research 46, 687–689.CrossRefGoogle ScholarPubMed
Rosso, P., Keyou, G., Bassi, J. A. & Slusser, W. M. (1981). Effect of malnutrition during pregnancy on the development of the mammary gland of rats. Journal of Nutrition 111, 1937–1941.CrossRefGoogle Scholar
Rothamsted Experimental Station (1994). Genstat version 5.3.1. Harpenden, Herts.: Rothamsted Experimental Station.Google Scholar
Sinha, Y. N., Anderson, R. R. & Turner, C. W. (1970). Growth of mammary glands of the golden hamster, Mesocriectus auratus. Biology of Reproduction 2, 185–188.CrossRefGoogle ScholarPubMed
Srivastava, L. S. & Turner, C. W. (1966). Restricted feed consumption and its effect on mammary gland growth. Journal of Dairy Science 49, 1050–1052.CrossRefGoogle ScholarPubMed
Sturnman, J. A., Devine, E., Resnick, O. & Morgane, P. J. (1986). Maternal protein malnutrition in the rat: effect on protein and two enzymes in milk. Nutrition Research 6, 437–442.CrossRefGoogle Scholar
Sykes, J. F., Wrenn, T. R. & Hall, S. R. (1948). The effect of inanition on mammary-gland development and lactation. Journal of Nutrition 35, 467–476.CrossRefGoogle ScholarPubMed
Tucker, H. A. (1987). Quantitative estimates of mammary growth during various physiological states: A review. Journal of Dairy Science 70, 1958–1966.CrossRefGoogle ScholarPubMed
Tucker, H. A. & Reese, R. P. (1962). Nucleic acid estimates of mammary tissue and nuclei. Proceedings for the Society of Experimental Biology and Medicine 111, 639–642.Google ScholarPubMed
Vonderhaar, B. K. (1977). Studies on the mechanism by which thyroid hormones enhance a-lactalbumin activity in explants from mouse mammary glands. Endocrinology 100, 1423–1431.CrossRefGoogle Scholar
Wilde, C. J. & Kuhn, N. J. (1979). Lactose synthesis in the rat and the effects of litter size and malnutrition. Biochemical Journal 182, 287–294.CrossRefGoogle ScholarPubMed
Williamson, D. H. (1984). The regulation of substrate utilization. Clinical Nutrition 2, 129–136.CrossRefGoogle ScholarPubMed
Winick, M. & Noble, A. (1965). Quantitative changes in DNA, RNA, and protein during prenatal and postnatal growth in the rat. Developmental Biology 12, 451–466.CrossRefGoogle ScholarPubMed